Sealant Microleakage in Saliva-Contaminated Enamel:Comparison between three adhesive systems

Saliva contamination is the main reason for the failure of sealant therapy. The aim of this study was to evaluate the effect of three types of adhesives (Single Bond, Adhes and I Bond) on sealant microleakage in saliva-contaminated enamel. In this experimental study, 54 intact premolars were chosen and divided into 5 groups. Group 1: teeth were etched and sealed with sealant. In groups 2, 3 and 4: teeth were etched and exposed to saliva for 10 seconds and air-dried. Subsequently, a bonding agent (Single Bond, Adhes and I Bond) was applied prior to the sealant application and curing. In group 5: after being etched and contaminated, the teeth were sealed without any bonding agent. After thermocycling, the teeth were immersed in fuchsin 0.5% as a leakage tracer for 24h.Then the teeth were sectioned in a longitudinal direction. Samples were then evaluated using stereomicroscope. The findings were statistically analyzed using Kruskal - Wallis and Dunn tests. The extent of microleakage in saliva contaminated sealant without bonding agent group had significant difference compared to the Single Bond, Adhes and I Bond groups)p<0.05). Although the amount of microleakage in study groups was less than the non–contaminated fissure sealant group, the difference was not significant. Besides, there was no significant difference between Single Bond, Adhes and I Bond groups. Exposing enamel to saliva after etching would increase the microleakage. Using Single Bond, Adhes and I Bond the dentin adhesives, after salivary contamination would decrease the microleakage to the extent of uncontaminated sealant group.