Effects of Scrophularia striata Extract on Wound Healing in Rabbit

Scrophularia striata which grows in Ilam province is used as a traditional medicine for treatment of diseases for years. Medical properties of this plant have not been documented yet. For this reason, a study was performed on this plant In study, the healing effects of Scrophularia striata extract was investigated on open skin wound of rabbit. All parts of the plant were collected and dried. Hydroalcoholic extract of Scrophularia striata was prepared by maceration method. 36 males and female Newzeland rabbits weighing 1.2-2.06 kg were used during the study. Method of Cross et al. was used to make full thickness wound (20x20) in left flank of the animals. They were divided into 6 groups: the first group was left without treatment, the second was treated with Eucerin, in third group Phenytoin cream 1% was used and in the other groups, different concentrations of hydroalcoholic extract of Scrophularia striata(2%, 5%, 10%w/w) in Eucerin base were administrated 2 times daily. The areas of wounds were measured daily and healing percentage during all the days was compared with one wat ANOVA and Turkey. For histopathological studies, some biopsies were taken on the 7th and the last day. In no treatment and Eucerin groups, the healing was completed in 21 days. In Phenytoin %1group, the healing time was 16 days and in Scrophularia striata groups of 2%, 5% and 10% the complete wound closure was observed in 18, 17 and 16 days respectively. There was a significant difference between all the treated, and control and Eucerin groups, but statistical and histological studies showed that the best healing effect was from Scrophularia striata 10% extract. However, considering histological examinations and macroscopic observations, it can be suggested that Scrophularia striata extract may stimulate collagen synthesis, faster wound contraction, angiogenesis, vessel dilatation and decrease of inflammation, bleeding and edema.