Isolation of Meloidogyne incognita Author race 2 from Olive in Golestan area

Root-knot nematodes are one of the limiting factors in seedling production and new olive gardens establishment in Iran. In view of find resistance cultivars to root-knot nematodes, importance of careful identification of species and races are clarified. In order to investigate root-knot nematodes on olive, soil and root samples of gardens and nursery in Gorgan area were collected. In this study, Meloidogyne incognita was identified based on females and second stage juvenils morphometrical and morphological characters. Stylet length, body length of female nematode, tail and hyaline length of second stage juveniles were shown some differences with original description. In original description, body length of females was 500 to 723 micrometer (609 micrometer average), whereas in under consideration species body length was (512-751) 622 micrometer. In original description tail length of second stage juveniles was (45-52.2) 48.7 micrometer and hyaline was (6.3-13.5) 8.9 micrometer while in investigated species tail length and hyaline were (43.9-55.4) 52.1 and (10.6-14.2) 12.9 micrometer, respectively. In response to differential hosts, the nematode showed a good multiply on tobacco, tomato, pepper and watermelon plants and able to produce knot whereas response cotton and peanut plants were negative. On the basis of morphological and morphometrical characteristics of matured female, perineal pattern, second stage juveniles, and response to differential hosts of M. incognita race 2 was detected.