Allelic and genotypic frequency of Calpastatin gene in Ghezel and Arkhamerino sheeps and their crossbreds

Calpastatin have role in regulation muscle growth and meat tenderness after slaughter that its coding gene located on ovine chromosome 5. Studies have shown that this gene is polymorphic in many breeds of sheep and is related with weight gain and carcass traits. This is the result of a single base pair substitution in the Calpastatin gene that is recognized by MspI and NcoI restriction fragment length polymorphisms (RFLP) method and can be use as genetic marker in animal breeding. The aim of this study was to analysis of genotype distribution of Calpastatin gene in sheeps by MspI/RFLP method. Blood samples were taken from 37 sheeps (65 Ghezel, 4 Arkhamerino and 30 their F crossbreds). Genomic DNA was extracted from 50ul blood sample. Gel monitoring and spectrophotometer methods were used to determination quality and quantity of DNA. Primers ovine Calp-R and ovine Calp-F amplified a 570 bp fragment of the ovine Calpastatin gene. MspI enzyme was used for restricting of PCR products. Digested products were separated by electrophoresis on. 5% agarose gel and visualized after staining with ethidium bromide on UV transilmination. Data analysis was done using PopGen3 software (ver..3). Frequency of M-allele in Ghezel, Arkhamerino and their crossbreds were 69%, 48% and 50%, respectively. The sheep populations were in Hardy-Weinberg equilibrium and it was concluded that breeding based on selection for Calpastatin gene was not done.