Improved Sorbitol MacConkey Agar Medium Containing Cefixime and Potassium Tellurite for Isolation and Diagnosis of E. coli O157:H7 from clinical case

250 fecal swab sheep slaughtered in slaughterhouses Shiraz to isolate the bacteria E. coli O157 was selected. Prepared SMAC plates containing from 0.05 mg L-1 ceffexime and 2.5 mg L-1 potasium tellurite as standard (1S) to 6 time standard (6S). Certain pure E. coli O157 EDL933 was plated on SMACs. All plates incubated in 37°C over night. E. coli O157 was growth on all six SMACs, but all plates had grown with the same count in 1S to 3S plats. Some bacteria decreased according to dose of antibiotics. Two and four percent contamination rate was shown high sensitivity in 3 S than 1 S. use of media with high antibiotic deleted extra intestinal bacteria. We recommend using ICT-SMAC medium supplemented with 1.5 and 7.5 mg L-1 ceffexime and potassium tellurte respectively in spite of current SMAC to isolate E. coli O157 from clinical case