Evaluation of Nano silver particle's cytotoxicity on L929 fibroblast cells by MTT assey: an in vitro Study

Antimicrobial effects of nano silver particles have received enormous attention in medical and dental applications. Despite of wide spread use of nanosilver there is not enough studies about its side effects on human. Some studies have exhibited cytotoxicity of nano-silver particles. This research was designed to investigate the cytotoxic effect of nano silver particles on L929 fibroblasts cells, by MTT assay. In this experimental study, nano silver particles with 5,10,20,30,40 and 50 ppm concentration were exposed to 10.000 L929 fibroblast cells then cell vitality was assessed after 2,24,48 and 72 hours by MTT method. The light absorption was evaluated by Elisa Reader and recorded. Data was subjected to ANOVA test for statistical analysis. In all concentration groups, vitality of cells were the least after 24 hours, but the vitality of cells increased after 48 hours. The increase after 48H was statistically significant (p<0.05). Also concentrations more than 20 ppm in 2,24 and 48 H were significantly cytotoxic for fibroblasts. Nano silver particles at concentrations less than 20 ppm after 72 hours did not have toxic effects.