Evaluation Spread of Ant (2′′)-Ia Gene in Aminoglycoside Resistant Escherichia Coli Isolated from Urine by PCR

Ëscherichia coli is the most prevalent etiologic agent of urinary tract infection. Ënzymatic inactivation of aminoglycosides by aminoglycoside-modifying enzymes is the main mechanism of resistance to these antibiotics in Ëscherichia coli. The aim of this study was detection of ant (2′′) -Ïa gene among aminoglycoside resistant clinical isolates of Ë. coli using PÇR method

276 clinical isolates of Ë. coli were collected، antibiotic susceptibility patterns of isolates were determined by disk diffusion method for gentamicin، amikacin، tobramycin، kanamycin and netilmicin paper disks considering ÇLSÏ principles. Çhromosomal DNÂ of the isolates was extracted using DNÂ extraction kits and PÇR method was used for detection of the ant (2′′) -Ïa gene

Results of disk diffusion showed that 24. 63%، 23. 18%، 21. 01%، 6. 15% and 3. 62% of Ë. coli isolates were resistant to tobramycin، kanamycin، gentamicin، netilmicin and amikacin، respectively. Ânt (2′′) -Ïa gene was detected in 47. 88% of Ë. coli isolatesÇonclusion: Because of high prevalence of resistance toward aminoglycoside antibiotics due to its transfer among bacteria by transferable elements such as transposons and plasmids، tracing of transfer routs among different bacteria is very important.