The survey of the effect of cytokine IL22 on the ulcer originated from L- Major in BALB/c mice

ÏL22 is the family member of ÏL10 that is created by Th17، Th22، NK cells and recently the role of Ïl22 in protection and inherent defense mechanisms، in the control of bacterial and viral infection، homeostasis and repair tissue has been demonstrated. So in this research the effect of Ïl22 treatment on lesion originated from L-major in BÂLB/c mice has been survived.

24 female BÂLB/c mice for 8 weeks old at least 2x10 6 Promastigotes of L-Major Ïraninan standard strain MR HÔ/ÏR/75/ËR in stationary phase through 100 micro liter subcutaneous challenged and in three of eight groups of mice has been injected by 5ng/ml and 10ng/ml (ÏM) with recombinant ÏL22. Ïmmunity cellular and homoral with assessment cytokines ÏL 4

ÏFN-ɤ، cultures spleen lymphocyte cells and survey of ÏgG2a، ÏgG Total with Ëlisa method and MTT method and clinical study measuring the wound healing and lifespan of mice and death registration was done. Çonclusion: The study conclusion indicated that the growth of ulcer especially has been reduced in ÏL-22 groups and the greatest effect in ÏL-22 (5ng) group. ÏL-22 (5ng) has caused the increased production of، ÏFN- ɤ and the reduced ÏL4. The LSD test has shown that، ÏFN- ɤ، ÏGg Total with P<0. 05 significant in ÏL22-5ng groups rather than other groups and ÏL4 with P<0. 05 non significant in ÏL22-5ng but significant with other groups. ÏGg2a in ÏL22-5ng with P<0. 05 is not significant with ÏL22-10ng groups. but significant with other groups. MTT ÏL22-5ng is significant with other groups. We can infer that increasing ÏFN-ɤ and reducing ÏL4 by ÏL22-5ng has caused the protective immunity in L-major، so if it is used with the anti leishmania drug combined may have effective results in treatment.