The effects of GnRHa, HCG and pituitary extract on biochemical parameters of seminal plasma in goldfish (Carassius auratus gibelio)

In this study، the effects of hormonal GnRHa، HCG and pituitary extract injection on biochemical parameters of seminal plasma (Na+، K+، Ca+، Mg2+، total protein، cholesterol and glucose) in goldfish (Carassius auratus) males were compared. Fish injected with hormonal GnRHa، HCG and pituitary extract with 10μg kg−1، 1500 IU/Kg−1، 3mg/Kg−1 respectively. There were a highly significant difference of seminal plasma Na+ among treatments (p<0/05)، as the highest value of Na+ observed in GnRHa and HCG treatment (202. 33±15. 04، 195. 66±10. 69 mmol/l respectively). There was a highly significant difference in K+ among treatments (p<0/01) as the highest value of K+ observed in control group (31. 40±1. 24 mmol/l)، but There were no significant difference in Ca+ and Mg2+ among treatments (p>0/05). There was a highly significant difference of seminal plasma total protein and glucose among treatments (p<0/05)، as the highest value of total protein and glucose observed in HCG treatment (0. 06±0. 001 g/dl، 0. 13±0. 04 mg/dl respectively). Also There were a highly significant difference of seminal plasma cholesterol among treatments (p<0/01)، as the highest value of cholesterol observed in HCG and pituitary extract treatment (0. 018±0. 001، 0. 020±0. 003 mg/dl respectively). The present study demonstrated that GnRHa، HCG and pituitary extract have different effects on biochemical parameters of seminal plasma in goldfish، and HCG have more effect on biochemical parameters of sperm than GnRHa and pituitary extract.