Genetic diversity of the Tilia sp. using seed protein electrophoresis

To evaluate the genetic variation of Tilia sp. in hyrcanian forest seed proteins of five populations including; Dalir, Chamestan, Band-bone, Valik-bone and Loveh were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Seed proteins profile with fruit morphology diversity were also investigated by discriminant analysis. Due to, three current types of fruits including rufous trichomes, prominent rib and without rib were selected. The different clustering pattern of individuals from same population, especially in Loveh in east of Hyrcanian forest, might be due to lack of germplasm exchange between the neighboring regions. Cluster analysis using data generated by SDS-PAGE bands seed protein patterns, clearly separated three fruit morphotypes. In general, this study proved relationship between the studied storage protein and fruits morphotype.