In vitro Culture Optimization of Capsicum annuum L

With the notice to a papper plant is one of the most important medical plants in Iran and hasn’t done a cansiderable research about direct regeneration, this study was designed. In this study were used Pepper’s hypocotyl and cotyledon explants. Direct regeneration of the explants on MS medium containing different amounts of auxin and Sytokenin were examined. For examine direct regeneration two capsicum annuum L. ecotype with two kinds of explant and five different growth regulator combinition was performed with factorial experiment in the form of randomized completely design with three replications. Most direct regeneration were obtained on MS medium containing 6 mg.l-1 BAP with 1 mg.l-1 IBA from hypocotyl explant. Rooting also was carried out in MS medium without hormones.