DETACHMENT, CULTIVATION AND DEFFRENTIATION OF BULGE STEM HAIR FOLLICAL CELLS TO NEURAL CELLS

Throughout life, stem cells make new cells in various tissues. Bulge region of hair follicle indicated as sources of stem cells for along times but little studies done in vitro to characterize rat bulge hair follicle stem cells. In this study bulge cells of rat hair follicle were isolated and were cultured, then morphological features of differentiated cells were studied. Also, rat hair follicle cells were assessed one and three weeks after cultivation. The result showed that two days after cell attachment to floor of plates, the cells initiate to proliferation and migration. Growth curve showed that the rate of cell proliferation at day 8 reaches its maximum level. After a week of cell culture, they had good power but after detachment were limited reproduced. Most cells in high cell density conditions and NT- 3 treated media differentiated to neural and glial lineage. Results showed that cultivated rat bulge cells, have high proliferative potential, and also could change to neural and glial like cells.