مارکرهای سطحی و میزان بقای پلاکتها در زمان ذخیره سازی در دمای ۴ درجه سانتیگراد؛ تحت تاثیر اکتانوات سدیم
نویسنده(گان):	وحید بغدادی، فاطمه یاری*، نرگس رضایی، محمد حسام رفیعی
نوع مقاله:	مقاله پژوهشی/اصیل
زبان:	انگلیسی
انتشار در:	مجله هماتولوژی و آنکولوژی کودکان، سال نهم شماره ۲ (پیاپی ۳۶، Spring ۲۰۱۹)
صفحات:	۱۰۵ -۱۱۶
نسخه الکترونیکی:	متن این مقاله در سایت مگیران قابل مطالعه است.

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate
Author(s):	vahid baghdadi، Fatemeh Yari*، Negar Rezaei، Mohammad Hessam Rafiee
Abstract:	Background Storage of platelet concentrates (PCs) at room temperature (20-24°C) limits its storage time to 5 days due to the destructive effects of platelet storage lesion (PSL) and bacterial contamination. Although prolonged storage of platelets (PLTs) at 4°C reduces the likelihood of bacterial contamination and PSL levels, it is accompanied by an increase in the clearance rate and changes in the surface markers of PLTs. The goal of this study was to evaluate the effects of sodium octanoate (SO) as a stabilizer on PLTs during storage at 4°C. Materials and Methods In this experimental study, PCs were divided into three portions and stored for 5 days at 3 different conditions, including 20-24°C, 4°C temperature, and 4°C in presence of SO. PLTs enumeration was performed using an automated hematology analyzer. To measure the metabolic activity and survival rate of PLTs, the water-soluble tetrazolium salt (WST-1) assay was performed. The activity of lactate dehydrogenase enzyme (LDH) was measured by a biochemical analyzer. Additionally, the levels of PLT glycoprotein Ibα (GPIbα) and CD62P (P-selectin) were measured on PLTs by flow cytometry technique. Results PLTs count was higher in SO-treated (4°C) PLTs than two other studied samples. Additionally, the viability was higher in the SO-treated PLTs than that in other groups. LDH amount was lower in the SO-treated PLTs than that in other groups (P>0.05). GPIbα expression was significantly higher in SO-treated PLTs than that other groups (P0.05). Conclusions SO could modulate the effects of cold temperatures on PLTs. Furthermore, we found that the survival of platelets was better maintained in the presence of SO at 4°C.
Keywords:	CD62P، GPIbalpha، LDH، Platelet، Sodium Octanoate
Article Type:	Research/Original Article
Language:	English
Published:	Iranian Journal of Pediatric Hematology and Oncology, Volume:9 Issue: 2, Spring 2019
Pages:	105 -116
Full text:	PDF is available on the website.