The determination of bovine tuberculosis is made with different tests and with certain limitations. Antigenic proteins can be extracted from Mycobacterium bovis culture that can improve the sensitivity and specificity of diagnostic tests. One of the targets of this study is to design an indirect ELISA. For this reason, purified Mycobacterium bovis AN5 antigen were utilized to distinguish bovine tuberculosis. Another goal is to review and compare the results of the system designed to Interferon-ɣ (IFN-ɣ) assay. Mycobacterium bovis AN5 antigens were isolated and purified. The selected antigen was then used to design the indirect ELISA system. The sera of 230 cows from different farms were tested with designed ELISA. Their results were at that point compared with interferon-gamma test as a corroborative diagnostic test. Correlation and independent study between variables were performed by Pearson and T test. The sensitivity and specificity of the designed indirect ELISA system were 77.8% and 80.7%, respectively. The p value for Pearson and T test was zero percent. Noteworthy lightweight antigens were purified that might analyze the disease. Due to the high sensitivity and specificity gotten, the ELISA system outlined for starting screening within the diagnosis of bovine tuberculosis.

In bovine tuberculosis caused by Mycobacterium bovis, cattle are known as the main host, and sheep are relatively resistant. Sheep become infected only when there is very close contact between cattle and sheep or when the mycobacterium is abundant in the environment. In the present study, a case of generalized tuberculosis was reported in the sheep of the Sanandaj slaughterhouse.The examined animal carcass was transferred from the slaughterhouse to the University of Kurdistan microbiology laboratory and was dissected under complete health safety conditions. In the carcass examination, necropsy lesions (generalized tubercular granulomas) were visible in different organs and mediastinal lymph nodes, bronchioles, mesentery, and liver portal system. These lesions were seen in the lymph nodes of different body parts, including the lung, liver, digestive system, reproductive system, bladder, and different muscles, including the peritoneal muscle, diaphragm muscle, and abdominal muscles.Diagnostic tests included clinical observations, Ziehl-Neelsen staining for Mycobacterium bacteria, and polymerase chain reaction (PCR) molecular technique. In Ziehl-Neelsen staining, Mycobacterium acid-fast bacilli were seen in red color on the pale blue background of the slide. Also, the product of 499 base pairs was amplified in the specific PCR reaction, which confirmed Mycobacterium bovis.The present report shows that Mycobacterium bovis strains present in the region can be transmitted to sheep and cause disease with severe clinical symptoms. This report is critical, particularly for mixed cattle and sheep farming. Since cattle and sheep are kept together in many breeding farms in Kurdistan province, special attention was paid to the possibility of transmission of bovine tuberculosis to sheep and humans.