The object of the present research was to study the prevalence of Trichomonas vaginalis(T.V) and Candida albicant(C.A) and to compare the two stains of Loffler(L) and Diluted carbol-Fuchsin(DC-F) for rapid diagnosis of T.V in 500 vaginal discharges referred to Medical Center of Robat Karim City. Patients & In this study, comparative diagnostic techniques including direct smears, staining methods and culture media were used. Our results indicated that the prevalence rates of Trichomonas vaginalis and Candida albicans in our defined society were 7(1.4%) and 67(13.4%) respectively. According to our study there were no significant relations between Trichomonas vaginalis infection and the patients’ age, level of education number of childbirth, use of contraceptive devices, vaginalis itch, pruritus and painful intercourse(P>0.05). However, there was a statistically significant difference between vaginal pH, vaginal discharges, WBC number in each microscopic field and Trichomonas vaginalis infection(P<0.05). No significant difference was found with regard to Candida albicans infection and women’s age, education level and the number of parturitions(P>0.05). However, contraceptive devices, vaginal discharges, itches and burning, painful intercourse, vaginal pH, number of WBC in every microscopic field were statistically significant(P<0.05). Using Loffler and Diluted Carbol Fuchsin for rapid diagnosis of Candida albicans was quite statisfying but not suitable for Trichomonas vaginalis.