This research was performed to introduce a standard protocol for enrichment of Artemia urmian with Canola oil. Artemia urmiana nauplii were enriched at three densities (50000، 100000 and 200000 nauplii L-1) and three concentrations of Canola oil (0. 1، 0. 2 and 0. 3 g L-1). Their effects were evaluated on survival، total length and profile of fatty acids at 6، 9، 12، 15 and 18 hours after the onset of enrichment. Cysts of A. urmiana were hatched according to the standard method. A. urmiana nauplii were stocked at above densities in 7 L cylindrical containers. Canola oil emulsion was added at concentrations of 0. 1، 0. 2 and 0. 3 g L-1 at the beginning and 12 hours after the onset of enrichment. The results of analysis showed that enrichment of A. urmiana with 0. 3 g L-1 Canola oil at 100000 nauplii L-1 for 18 hours was considered as the best treatment. Artemia nauplii enriched in this treatment had significantly higher levels of (n-3) PUFA and survival and minimum total length comparing to other treatments. The treatment had significantly higher levels of (n-6) PUFA than all treatments except treatment with a density of 50،000 nauplii L-1 with 0. 1 g L-1 Canola oil for 18 hours.

In this study¡ the effects of enriching Artemia franciscana nauplii with three concentrations of Canola oil at three density levels were evaluated on survival¡ total length and fatty acids of Artemia franciscana nauplii to determine the optimum concentration oil¡ density of nauplii and optimum time for maximum survival rate and fatty acids and minimum total length of nauplii. Cysts of Artemia franciscana were hatched according to the standard method (Sorgeloos¡ 1986). Artemia franciscana nauplii were then stocked at densities of 50000¡ 100000 and 200000 nauplii L-1 in 7 L cylindrical containers. Canola oil emulsion was added at concentrations of 0.1¡ 0.2 and 0.3 g L-1 at the beginning and 12 hours after the onset of enrichment. Nauplii were sampled at 6¡ 12 and 18 h after onset of enrichment for measuring the survival¡ total length and the concentration of fatty acid. The results of analysis showed that enrichment of Artemia with 0.3 g L-1 Canola oil at 50000 nauplii L-1 for 18 hours was considered as best treatment. Artemia nauplii enriched in this treatment had maximum linoleic and linoleic acids¡ highest survival and minimum total length.

Fire blight disease caused by Erwinia amylovora is deemed to be one of the most important and devastating disease affecting in Rosacea. Sensitive and rapid detection protocols are important tools in disease control. In this research sensitivity and specificity of four different detection methods with and with out enrichment were evaluated. 1CFU/ml E. amylovora could be detected by selective culture method in infected plant extract but not in latent infections. However, enrichment was increased the susceptibility of this method to detect E. amylovora in latent infections. Lateral flow immunochromatography detect 105 CFU/ml bacteria in infected plant extract and enrichment made this method to detect latent infections and increased the sensitivity to detect 1CFU/ml bacteria. Using specific primers, the standard and nested PCR were detected 1CFU/ml bacteria in infected plant material.

Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices. Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx2 in E. coli O157:H7 without prior enrichment was 103 CFU/ml, and with 4 h prior enrichment in modified tryptic soy broth was 102 CFU/ml. During a period of 7 months (December 2004 to June 2005), 154 slaughtered cattle at Shiraz slaughterhouse, were randomly selected and examined for surface carriage of E. coli O157:H7 via detection the Stx2 gene of verotoxigenic E. coli by PCR technique. E. coli O157:H7 was found in 14 of 145 (9.65%) feedlot cattle and in 1 of 9 (11.1%) dairy cull cows. This is the first report of the presence of E. coli O157:H7 in cattle from Iran.

High reactivity of uranium hexafluride compound is responsible for the impurity production of hydrogen fluoride in the enrichment industry process. The presence of this interference gas will disrupt uranium enrichment process. For this reason, measurement of hydrogen fluoride gas in uranium hexafluoride matrix as one of the important factors in the enrichment industry was carried out by FTIR spectrometry. Using of a new manifold system and a 10 m pathlength reflectance gas cell can lead to perform the analysis of hydrogen fluoride in the easier and more appropriate conditions than the ASTM method. The results obtained from the optimization of the present method are as flow: reduction of utilized UF6 (≈ 80%), elimination of liquid N2 for sample preparation, and simplification of sampling. Among the five standards and international regulations available, the ISO/IEC 17025 standard considerations is used, and verification parameters such as linearity, accuracy, precision, detection limit, quantization limit, and uncertainty of the results for the effect of the existing errors were studied.

Platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. Platelet concentrates can be prepared by the apheresis method or from the whole blood using the Buffy-coat or Platelet-rich plasma methods. The purpose of this study was to compare four variations of platelet-rich plasma method based on double centrifugation protocol to identify the optimal centrifugation conditions with greatest platelet recovery and highest enrichment capacity for preparing platelet concentrates. Blood samples were obtained from 145 donors, chosen randomly from the donation department at the Tehran Blood Transfusion Center, Tehran, Iran. Four variations of platelet-rich plasma methods were selected for preparation of platelet concentrates. Platelet counting analysis was performed on samples and platelet enrichment and platelet recovery were calculated by investigating the correlation between the number of platelets in the whole blood, platelet-rich plasma and platelet concentrates. Platelet count analysis revealed that the method performed with 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step had the highest platelet enrichment (5.59fold) and greatest platelet recovery (78.63%). Within the limits of this study, it can be concluded that 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step yielded the greatest platelet recovery and highest enrichment capacity and is a good choice for preparing platelet concentrates. Keywords: Platelet, concentrates, plasma, centrifugation, recovery, enrichment.

Heavy metal pollution is one of the most serious environmental issues globally.In this research we were studied the heavy metal concentrations in sediment and fish in Persian gulf.
Materials & Methods For determination of heavy metal concentrations in sediments 8 sampling stations were selected to measure Cd, Zn, Ni, Pb and Fe in the sediments and in the muscle tissue of Otolithes ruber from the northern part of the Hormuz strait )Persian gulf). Samples for metal analysis are prepared according to MOOPAM. Heavy metal concentrations were analyzed using inductively coupled plasma-optical emission spectrometry (ICP-OES)
Analysis of the potential ecological risk of sediment heavy metal concentrations showed that most sample sites in the northern part of the Hormuz strait (Persian Gulf) presented a low ecological risk. Regarding enrichment of Cd, Zn, Ni, Pb and Fe the highest EF belongs to Pb. The obtained mean enrichment factor (EF) values for various metals were between no enrichment and moderate enrichment.
The concentrations of heavy metal in the edible part of O. ruber did not exceed the permissible limits proposed by NOAA, FAO and WHO standards and are suitable for human consumption, except for Pb and Cd.

A highly sensitive, simple, and speed technique was employed for the determination of brilliant green in fish (Sphyraena jello) and seawater samples by visible spectrophotometry after its extraction and enrichment with chitosan- zinc oxide nanoparticle coupled with dispersive liquid-liquid microextraction. Ultra-trace concentrations of brilliant green were dispersed to the organic phase in DLLME method after adding dispersive solvent and chitosan- zinc oxide nanoparticles. The experimental factors such as the amount of chitosan- zinc oxide nanoparticles, the concentration of Triton X- 114, type of volume of extraction and dispersive solvents, extraction time, rate and time of centrifugation, the volume of sample, and pH were investigated to order to enhance of the extraction efficiency. Under optimum extraction conditions, the volume of chloroform (as extraction solvent) and methanol (as dispersive solvent) were 100.0 µL and 550.0 µL, respectively; the amount of chitosan- zinc oxide nanoparticles was 15.0 mg; the time of extraction was 4.0 min; rate and time of centrifugation were 3000.0 rpm and 8.0 min, respectively, the volume of sample was 8.0 mL, and pH of the sample solution was 4.0. After optimizing the microextraction conditions and instrumental factors, an enrichment factor of 169.0 was achieved. The analytical curve )absorbance vs. concentration( was linear over the range 1.0-200.0 µg/L of brilliant green. The limit of detection and relative standard deviation were 0.3 µg/L and < 6.1 %, respectively. The protocol was successfully employed for the determination of brilliant green in seawater of Chabahar Bay and fish (Sphyraena jello) samples.

The aim of the present study was to compare the effectiveness of schema therapy based on enrichment programs and systemic-integrated couple therapy on interpersonal forgiveness of betrayed women. The method of the current research was semi-experimental with a pre-test-post-test design and a control group with a follow-up period of two months. The statistical population of the present study was made up of all the betrayed women who were referred to the counseling centers located in Rodhen City in 2022. 45 people were selected in a purposeful way and randomly assigned to two experimental groups and one control group. The instrument of this research was Pollard's interpersonal forgiveness questionnaire (IFS) (1998). Schema therapy intervention based on Lu and McKay's acceptance communication enrichment program (2012) and Feldman's (1990) system-integrated couple therapy protocol was implemented in 12 90-minute sessions twice a week for the first and second experimental groups. The data were analyzed using mixed analysis of variance. The results of the Varbans analysis showed that there is a significant difference between the groups in the post-test and follow-up of interpersonal forgiveness (P<0.05). The results showed that the schema therapy based on the communication enrichment program of acceptance compared to Feldman's systemic-integrated couple therapy had a significant effect on forgiveness The interpersonal relationship of betrayed women was more effective. A significant difference was observed between the two treatments (P<0.05). It can be concluded that there is a substantial difference between the two treatments of schema therapy based on the enrichment of acceptance communication and systemic couple therapy.

Tradeoffs between security and performance are the most important issue in wireless networks. An authentication protocol is a type of cryptographic protocol with the purpose of authenticating entities. Latest standards for re-authentication protocols have published by Internet Engineering Task Force (IETF). In this research, after reviewing some protocols in this scope, a security protocol is proposed. The proposed protocol is based on IETF standards. Fundament of RFC 6696 is exploited to develop the proposed protocol. It offers serious advantages over the existing IEEE 802.1X standard protocols, including: symmetric cryptosystem, challenge–response and hash chaining.