نشریه اکوفیتو شیمی گیاهان دارویی
سال هفتم شماره 2 (پیاپی 26، تابستان 1398)

Tomato early blight disease, caused by the fungus Alternaria alternata, is one of the most devastating diseases for the crop in Iran and worldwide. Recent studies have shown that some secondary metabolites of medicinal plants are effective in preventing the growth of fungi and are a good alternative to the use of chemical pesticides. In the present study, ten medicinal plants including Eucalyptus sp., Thymus vulgaris, Mentha piperita, Mentha longifolia, Mentha aquatica, Satureja hortensis, Achillea millefolium, Foeniculum vulgare, Anethum graveolens, and Trachyspermum copticum were collected during 2016-2017. Their essential oils were extracted using Clevenger apparatus, and GC-MS device was used to identify the essential oil compounds. Antifungal activity of essential oils was assayed through the inhibition of fungal colony growth at five concentrations of 0, 200, 400, 800, and 1200 ppm in mixing with potato dextrose agar medium with three replicates. The findings revealed that fungus colony growth was inhibited with increases in essential oils concentrations, and there were significant differences among different essential oils. Essential oils of Satureja hortensis, Trachyspermum copticum and Thymus vulgaris in 800 and 1200 ppm and Anethum graveolens in 1200 ppm demonstrated the highest effect on fungus colony growth. This study found that Carvacrol, Thymol, Para-cymene, and Gamma-terpinene ingredients tend to have the highest antifungal effects.

In this study, aerial parts of Artemisia persica in blooming satage were collected from Noor Abad Research Farm (Lorestan province, Iran) in the summer of 2017. Essential oils of plants were extracted by water distillation and supercritical fluid extraction (SFE) methods and were analyzed by using GC/MS device. Antifungal properties of essential oils were evaluated on Microsporum canis, Trichophyton rubrum, Epidermophyton floccosum, and Trichophyton mentagrophytes strains by broth microdilution method. Antioxidant activity of the essential oils was also evaluated by two free radical scavenging tests, viz. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Essential oil yields were 1.7% and 1.7-5.7% in water distillation and SFE methods, respectively. Major compounds identified in the SFE method were Davanone (29%), lacintafuran H (18.1%), C-artedoglacia oxide (8.5%), and neurodevnet (7.6%). Davanone (22%), lacintaphoran G (7.1%), and D-artedoglacia oxide (6%) were the predominant compounds detected in water distillation method. The essential oil extracted by water distillation had the highest antifungal effects on E. floccosum and T. montagrophytes strains with MFC and MIC of 32 µg/mL. Water extracted essential oil presented the highest free radical scavenging capacity in DPPH and ABTS tests with IC50 values of 20.32 and 14.23 μg/ml, respectively.

The present study aimed to optimize the callus induction and to determine the expression of flavonol synthase and chalcone isomerase genes in the pathway of quercetin production under UV-B rays in bitter cucumber callus cultivation. In this research was used concentration and combine of plant hormone 2, 4-D and BAP and explant to callus induction in Momordica charantia using complete random design with three replication. Genes expression study of Quercetin biosynthesis pathway such as FLS, CHI (were analyzed by 2-∆∆CT) and antioxidant activity assay of phenylalanine ammonia-lyase (Produced by concentration of cinnamic acid) and flavonoid using best callus were selected and affected by UV-B ray (five group(Control(natural light), 5 minute under UV-B ray(studying of gene expression after 24 and 48 hour) and 10 minute under UV-B (studying of gene expression after 24 and 48 hour)). Analysis of variance was shown that kind and concentration of plant hormone was significant on callus induction. Mean square of data was shown that the most callus dry weight was obtained from 0.5 mg/L 2,4-D and 1 mg/L BAP and stem explant. The highest expression of flavonol synthase and chalcone isomerase (chi) was observed in the 10-min UV-B treatment at 24 h after treatment but decreased at 48 hours after treatment. The highest expression of PAL, quercetin and flavonoid were obtained at 5 minutes UV-B treatment at 48 hours after treatment. Overall, the results showed that UV-B radiation at the callus level had a positive effect on the expression of flavonoids biosynthetic pathway genes.

Biotransformation is the process of converting an inert precursor into the medicinally valuable product. In this research, a pot experiment was conducted to evaluate the effect of artificial citral and geranial spraying in concentrations of 100, 200, 300, 400 and 500 ppm, besides distilled water and without spraying (control) on essential oils quality and quantity of lemon verbena with in greenhouse conditions at Islamic Azad University – Miyaneh Branch, Iran in 2015. The first foliar application was done when the plantlets reached the height of 25. The second and the third spraying were done with 7 and 14 days span after the first spraying respectively. One week after the last spraying, lemon verbena leaves were harvested. The essential oils were extracted from the leaves by hydro distillation method by using Clevenger apparatus. Citral and geranial especially in low concentrations (100 ppm) and distilled water spraying had positive effect in essential oils yield. The chemical composition of essential oils was analyzed by GC and GC-MS methods. On the whole, 21 components were identified and the most important components were limonene, geranial and neral. Citral and geranial spraying especially in high concentrations increased limonene content and the highest limonene content was in geranial 500 ppm and citral 400 ppm in the amount of 28.24 and 24.54%, respectively. Also, the lowest limonene content was 17.83% in control. However, citral and geranial spraying had not positive effect on neral and geranial content.

Glycyrrhiza glabra L. is a family of Legominos, herbaceous, perennial and Iran is one of the countries exporting its root. This plant grows in different regions of Iran, hence the study of phytochemical diversity in different regions is important. This study was carried out to evaluate the content of phenolic compounds and antioxidant activity of 2 cm diameter roots of licorice populations in 15 provinces and 30 regions of Iran in 2017 in October and were transferred to the laboratories of the Department of Horticulture of Zanjan University. Total phenol (Folin–Ciocalteu method), total flavonoid (Aluminum Chloride method), anthocyanin (pH difference method) and antioxidant activity (DPPH method) were studied. The results of analysis of variance of data showed that among 30 populations, for all traits, there was a significant difference in the level of one percent. Total phenol traits ranging from 456.05 to 826 mg gallic acid per 100 g, total flavonoid between 1909.25 to 292.62 mg of quercetin per 100 g, anthocyanin levels ranging from 6.89 to 26.24 mg cyanidine-3-glucoside in Liter, the antioxidant activity varied between 62.07% and 87.14%. There was a significant and positive correlation between phenol total phenotype and antioxidant activity at 1% level, but there was no significant correlation with total flavonoid, anthocyanin and height. Based on the results of cluster analysis, 30 G. glabra were divided into two main groups. The evaluation of populations in terms of phytochemical traits showed a high variation that N, KA, BA, T, E, Y, M, MR, and SB populations

Due to unawareness of production method, improper uses and too much harvested this plant (Salvia mirzayanii ) natural of southern arenas, it seems that developing plans for its production is necessary. The experiment was carried out in the field research of Jahad Agricultural Center of Larestan, Fars province duoring winter 2017. The effects of different organic fertilizers including sheep manure (20 and 40 ton/ha), cow manure (20 and 40 ton/ha), compost (10 and 20 ton/ha), vermicompost (20 and 40 ton/ha) and control on physicochemical and quality traits Salvia mirzayanii were investigated in a complete random block design with four replications. Early in April, all vegetative organs of plants were harvested and dry and fresh weight of plantwere measureal. Also, the amount of total antioxidant, phenol and flavnoide of leaves were determinal spectrophtometi while essential oil percentage were determined distillation method with water and by Clevenger apparatus. The results showed that highest fresh and dry weight of the plant was obtained from the treatment of 10 ton/ha of compost. The highest amount of antioxidants, phenol and flavonoid total were observed in 40 ton/ha of cow manure treatment. Among applied the treatments only 10 ton/ha vermicompost significantly increased the amount of essential oil compared to control. In general greater fresh and dry weight of plant increased by compost fertilizers. Effect of manure on phytochemicals was higher than compost, and essential oil value of plant increased only by vermicompost application

Cadmium is one of the most dangerous heavy elements that naturally or by human activity enter to the soil and causes oxidative stress in plants. Therefore, this study was conducted to evaluate the effect of cadmium on some of the physiological responses of three aromatic plants including water mint (Mentha aquatica L.), eryngo (Eryngium caucasicum Trautv.) and froriepia (Froriepia subpinnata Ledeb). In three experiments greenhouse, five concentrations of cadmium in soil containing 0, 5, 10, 15 and 20 mg cadmium/kg soil was investigate in a completely randomized design with four replications at Sari University of Agricultural Sciences and Natural Resources, 2017. Seedlings of the all three plants were planted in pots of cadmium contaminated. In before flowering stage Chlorophyll a, b, carotenoid, phenol, flavonoid and antioxidant capacity of shoots were measured. Results showed that leaf pigment in the three all plants decreased linearly with increasing cadmium level, but carotenoid have the lower reduction of compared to chlorophyll a and b. Also chlorophyll b was more sensitive than chlorophyll a to cadmium. While, flavonoid, phenol and antioxidant capacity of plants increased with increasing cadmium levels. Water mint had the average highest phenol (65.89 mg/g), flavonoid (146.88 mg/g) and antioxidant activity (98.49%) than other two plants. Overall, the results showed that plant pigments were affected by cadmium toxicity, and as these plants are rich in antioxidant compounds, the increase of these compounds with increment of cadmium stress is a kind of defence mechanism of three plants for conflict with stress.

In order to investigation of effect of iron, zinc, manganese and copper on morphological and phytochemical traits of Matricaria recutita L, an experiment was conducted as randomized complete block design with three replications during 2017 on the Research Field of Payam Noor University of Marand. Experimental treatments consisted of control, foliar application of iron, zinc, manganese, and copper. Results showed that foliar application had significant effect on all studied traits. Application of micronutrients had positive effect on studied traits compared to control treatment. The highest values of plant height, number of flowering branch per plant, number of flower per plant, yield of dry flower, total dry weight, harvest index, chlorophyll content, essential oil percent and essential oil yield were gained of foliar application of iron. The total chlorophyll content increased significantly due to the application of micronutrient elements compared to control treatment. Foliar application of Fe more than twice increased essential oil yield compare to control treatment. The highest and the lowest essential oil yield (1.93 and 0.9 kg/ha) was gained in application of Fe and control treatments respectively. Foliar application of iron treatment (383 kg/ha) increased yield of dry flower percent up to 30% compare to control treatment (258.63 kg/ha). Non application of micronutrients decreased all of the studied traits in plants. In general, results showed that foliar application of iron can be improved quantitative and qualitative yield of Matricaria recutita L. which could be used as a suitable management strategy for reach to optimum yield.

The researchers have, recently considered using plants and their active components in the synthesis of metal nanoparticles. In this project, the aerial part of Portulaca oleracea L. (Portulacaceae) before the flowering stage, was collected from Roodsar (Gillan province) on June 2017 and the aqueous extract was prepared by warm maceration method. Aqueous extract of the aerial parts (leaves and flowers) of the plant and Ferric chloride solution was heated at 70°C for 3.5 h. and Iron oxide nanoparticles with average size of 90 nm were synthesized, by the co-precipitation method. Formation of the Iron oxide nanoparticles was confirmed by Ultraviolet-Visible spectroscopy (UV-Visible), Fourier Transform Infrared Spectroscopy (FT-IR) and X-Ray Diffraction Analysis (XRD) techniques. The absorption peak at 288 nm confirmed the formation of nanoparticles and FT-IR spectra were showed the presence of organic compounds around the nanoparticles. XRD results showed the phase of hematite (α-Fe2O3) for the synthesized nanoparticles. Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX) confirmed the formation and purity of the nanoparticles. TEM image showed that the synthesized Iron oxide nanoparticles have a spheroid/cube geometry. The nanoparticles were stabilized by the organic compounds of the extract and were not agglomerated. These organic compounds can also improve the capacity of the nanoparticles in environmental remediation.