Journal of Skin and Stem Cell
Volume:4 Issue: 3, Dec 2017

Cell therapy, with autologous dermal fibroblasts, shows remarkable potential and is currently achieved by different methods, such as spraying, grafting, and injecting to treat various types of ulcers, wrinkles, acne scars, and surgical wounds.

To prepare fibroblasts for clinical use, some critical points must be taken into consideration: isolation and culture of these cells to produce a homogeneous population of non-contaminated cells, a method for rapid growth, and development of a proportional number of fibroblast cells with proper strength and viability. In addition, an appropriate site for a biopsy should be selected based on indications of treatment and maintenance.

Ultimately, the clinical use of fibroblasts in wound healing obviates the need for ready to use reservoir of these cells. In this regard, dermal allografts can be used in both fresh and frozen forms. The fresh type is more desirable due to its high chance of survival. Developing the appropriate preservation methods for allogeneic fibroblasts is the emerging research field of interest. Furthermore, applying biomaterials will increase the efficacy of fibroblasts in wound healing.

Basal cell carcinoma is the most common cancer in humans. The most common cause of this cancer is ultraviolet radiation.

The aim of this study was to investigate a possible relationship between the levels of vitamin D and basal cell carcinoma.

In this case-control study, 70 patients with basal cell carcinoma were admitted and registered in the dermatology clinic of Razi hospital in Rasht. The control group included healthy people from the skin clinic or hospital staff matched for gender, skin type, and age. Patients not receiving calcium supplements or vitamin D and not having underlying conditions associated with vitamin D deficiency were included in this study. The individuals were questioned to complete a demographic questionnaire. Blood samples were taken from all the participants and analyzed for vitamin D, parathyroid hormone, calcium, and phosphorus levels in a reference laboratory. Descriptive statistics and chi-square tests were used to analyze and evaluate the data.

The mean vitamin D level was 24.1 ± 12.1 ng/mL in the patient group and 24.6 ± 11.5 ng/ mL in the control group. Among participants in this study, 49 (35%) suffered from vitamin D deficiency, 62 (44.3%) had insufficient vitamin D levels and only 20.7% had acceptable vitamin D levels. There was no significant difference in vitamin D deficiency between the two groups including controls (34.3% (n = 24)) and patients (35.7% (n = 35)) (P = 0.1). Also, there was no significant difference between the two groups in terms of other biochemical factors in the blood.

The present study indicates that vitamin D deficiency is highly prevalent in both patients with basal skin cancer and healthy people, which should importantly be considered in future studies

Hypertrophic scars (HSC) and Keloids are fibroproliferative dermal lesions resulting in excessive accumulation of extracellular matrix in the dermis and subcutaneous tissue. Corticosteroid, especially triamcinolone acetonide, injections may be the first line treatment for the prevention and treatment of keloids and HSC. Some drugs that inhibit calcium channels, such as verapamil, have also been effective in treatment of these lesions. The present study aimed to assess and compare the efficacy of these two treatment options in patients with HSC and keloids.

The study used a randomized, single blind, parallel design to compare the effect of verapamil in comparison with triamcinolone on the healing of HSC and keloids in two groups comprising 25 patients. Patients were randomly allocated using random numbers to receive intralesional injection of 1 mL of either verapamil (2.5 mg) or triamcinolone (20 mg) every 3 weeks for 3 months.

Downward trends in all lesion parameters including height of lesion, as well as the scores for erythema, pigmentation in both groups was revealed. The change in characteristics of the lesion within the treatment was independent to patients’ gender and age. Regarding drug - related complications, one case of atrophy was reported in triamcinolone group, however, not in the verapamil group.

Both verapamil and triamcinolone have significant therapeutic effects in decreasing the height of the lesion, improving erythema, improving the pliability of the lesion, and improving the hyperpigmentation. It seems that verapamil has less cost and side effects in comparison with triamcinolone, thus, it could be a good choice in treatment of keloids.

Previous in vitro studies showed that sesame oil stimulates fibroblast production in wounds. Many studies investigated the therapeutic wound healing properties of natural products. The current study aimed at evaluating the effect of the combination of sesame oil and Cucurbita as a novel herbal mixture on healing the local wounds caused by the third- degree burn.

The current experimental study was conducted on 24 adult BALB/c mice randomly divided into experimental groups. Then, all mice were sacrificed and a sample of wound bed was provided and fixed in formalin, followed by the practical histologic process. The samples were stained with the Mallory-Trichrome technique and investigated under microscopy. A Part of the sample was frozen to extract protein, perform malondialdehyde (MDA) test, and measure total antioxidant.

A complete tightening of the wound and less scar was observed in the macroscopic observation of burn wounds in the group treated with sesame oil and pumpkin (treatment group 5) compared with the control and other groups. In the microscopic assessments, a significant increase (P < 0.05) was observed in the total thickness of skin, and number and diameter of the hair follicles in the experimental group compared with other groups.

The current study results showed that a combination of sesame oil and pumpkin at different concentrations accelerated the healing process of burn wounds in BALB/c mice, while using the under study materials separately had less effects on the improvement of skin wounds compared with the combination form.

Epidermolysis bullosa (EB) pruriginosa is a rare distinct clinical subtype of dystrophic EB, in which skin fragility, blistering, and scar formation are associated with intense pruritus, nodular prurigo like lichenified lesions, nail dystrophy, and variable presence of papuloid lesions.

Herein, we report a case with a history of blistering since infancy followed by intensely pruritic papulonodules, predominantly on the shins, nail dystrophy, and milia formation.

Our patient had an excellent response to systemic cyclosporine treatment