فهرست مطالب

Novelty in Biomedicine
Volume:9 Issue: 3, Summer 2021

  • تاریخ انتشار: 1400/06/08
  • تعداد عناوین: 8
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  • Abdolreza Javadi*, Bahareh Behjoo, Homa Forouhesh Tehrani, Houman Vosough, Kazem Namadmal Torkashe, Masoumeh Foroumand, Kavian Barzegarian Pages 111-117
    Background

    Antibiotic resistance is an important cause of treatment failure and re-infection in enterococci. In this study, the frequency of phenotype and genotype of Van A-B genes in Vancomycin resistant enterococcus isolated from a clinical sample of Imam Hossein Hospitals in Tehran was determined.

    Materials and Methods

    In this descriptive cross-sectional study in 2018, a total of 76 vancomycin-resistant enterococci in Imam Hossein Hospitals in Tehran were evaluated, including those from blood, urine, sputum, and wound. The frequency of phenotype and genotype of Van A-B genes in them was determined by MIC Epsilometer test and Multiplex Real-time PCR.

    Results

    The 160 isolates of enterococci collected from different hospital wards revealed that 76 (47%) enterococci were resistant by applying MIC E-test; interestingly, all VRE isolated showed high-level vancomycin resistance. The Real-time-PCR assay demonstrated vanA gene in 76 (100%) VRE isolates. Considering to controls no van B gene was detected in this assay. Based on bacterial phenotype tests, the results showed that 82% and 18% of the isolates were E. faecium and E. faecalis, respectively.

    Conclusion

    Totally, it may be concluded that Van A gene is more accompanied by high-level Vancomycin Teicoplanin resistance in common enterococci species. The frequency of Vancomycin resistance entrocooci is increasing especially among ICU admitted patients. For effective treatment MIC test and Van A-B genotyping seem to be essential.

    Keywords: van A, van B, Vancomicin resistance entrococci, Real-time PCR
  • Kourosh Eftekharian*, Hassan Eftekhar Ardebili, Sepehr Eftekharian, Ali Eftekharian Pages 118-122
    Background

    We aimed to evaluate Iran's current COVID-19 infection, emphasizing the number of infection detection and the disease's reproductive number in its high peak in November and after the lockdown in December.

    Materials and Methods

    Using the Johns Hopkins Bloomberg School of Public Health Contact Tracing Evaluation and Strategic Support Application, we used the average weekly new cases and average case mortality in November and December 2020. The average case isolation and identification time (25%, Four days) and the average case contact within the household and community were entered into the application. We examined Two modeling systems with 50% and 70% case isolation for the November period as alternative scenarios for the current infection control rate.

    Results

    Our modeling showed only 11% and 30% of the infections were detected in November and December. The disease's reproductive number is similar to the natural reproductive number of the disease (2-3) in November. The two models used to increase the rate of case isolation to 50% and 70% did not significantly change the reproductive number.

    Conclusion

    The priority in Iran for COVID-19 infection control should be a dramatic increase in the number of testing to achieve the correct number of case detection and fulfill the contact tracing criteria to reduce the disease spread.

    Keywords: Basic Reproduction Number, COVID-19, Quarantine, Contact Tracing, COVID-19 Testing
  • Ahmad Borjali*, Hosein Eskandari, Faramarz Sohrabi Asmroud, Yahya Mohajer, Davood Namazi Pages 123-131
    Background

    Identifying ethnic and personality facilitation factors in drug use help to better diagnose and treat addiction identifying facilitation factors. It provides timely counseling, and psychological services can be partially preventable for addicts. This study aimed to investigate factors facilitating drug use based on ethnic and personality characteristics of Guilan province using the Cloninger personality system.

    Materials and Methods

    The study population consisted of four subcultures of Guilak, Talesh (Turk), Kord and others called Fars. The sampling method in this study was a mixed-method; the sample size was at least 200 people. The present study used data in the field section of Cloninger's personality and character questionnaire to determine personality components and their relationship. They were used with current norms. This study used in-depth interviews, observation and review of documents (questionnaires) and conventional oral literature on drug use.

    Results

    This study showed that between ethnic facilitation factors (drug and drug counseling, folk beliefs in oral literature, and beliefs about rituals and companionship) and personality traits (nature and character), except the avoidance factor at the significant level of 0.01, there was a significant and positive relationship. Variables of personality traits/"nature and character" and components of perseverance, novelty, reward-dependence, self-direction, cooperation can predict common beliefs in the propensity to medication and therapy. The personality facilitator variable of "nature and character" and all its components, except for themselves, was able to predict the popular beliefs existing in oral literature. The personality facilitator variable of "nature and character" and the components of novelty, reward-dependence, and cooperation could predict common beliefs about rituals and companionship.

    Conclusion

    Research showed a significant relationship between ethnicity and propensity for drug and drug counseling, popular beliefs in oral literature, and celebration and celebration beliefs.

    Keywords: Drugs, Personality Traits, Ethnic Traits, Medication, Therapeutic Recommendations
  • Delsuz Rezaee, Sara Hosseini, Vahid Jajarmi, Mohammad Salehi * Pages 132-137
    Background

    Transgenic animals are genetically modified animals to create a specific trait that imitates an indication of pathogenesis in humans. Toll-like receptors (TLRs) are implicated in immune regulation of the female reproductive tract and, subsequently, infertility rate. This study produced Toll-like receptor 4 (Tlr4) knockout blastocysts with single-guide RNA targeting for Tlr4 by CRISPER/Cas9 technique.

    Materials and Methods

    Web CRISPER design tools designed single-guide RNAs (sgRNAs) targeting Tlr4 gene were designed by web CRISPER design tools. Then, two strands of sgRNAs were cloned into a linearized vector for producing a gRNA-expressing eCAS9-GFP vector. The vector was then injected into the male pronucleus in the fertilized oocytes in vitro fertilization (IVF) and do polymerase chain reaction (PCR) and sequencing.

    Results

    Gene deletion with acceptable efficiency (38%, p<0.05) successfully was confirmed by sequencing and PCR analysis.

    Conclusion

    Our result showed that the CRISPER/Cas9 technique is an effective knockout method in mouse zygotes, potentially producing disease animal models.

    Keywords: Transgenic, sgRNAs, CRISPER, Cas9, Toll-like receptors, Knockout
  • Delsuz Rezaee, Hanieh Rezaee, Saiyad Bastaminejad, Yadollah Bahrami*, Mohammad Salehi Pages 138-144

    The Coronavirus is a major health problem nowadays, which affects people's lifestyle. This pandemic virus shared a variety of phenomena in case of symptoms and side effects. One of the major issues regarding novel coronavirus is the effect of infection on pregnancy which accounts for an essential process of human life. Considering the pathogenesis of Coronavirus, overexpression of inflammatory cells and cytokines accounts a pivotal step in the development of symptoms. The over-expressed cytokines in response to covid-19 infection would render the inflammation and disruption of the immune system and tissue damage. Like coronavirus infection, implantation the main step of a successful pregnancy, activates the inflammatory cells and cytokines. The association of infection with pregnancy raises the concern about the effect of covid-19 on embryos and giving normal birth, especially in women who decide to get pregnant or are in the pregnancy period. The current review focused on immune system responses to the Coronavirus and comparison with immune system activation during implantation. It concluded that further laboratory research and studies are needed to better understand and draw general conclusions about the role of the virus in embryo implantation.

    Keywords: Coronavirus, Implantation, Embryo, Immune response
  • Houman Teymourian, Firoozeh Maddadi, Shayesteh Khorasanizadeh, Atieh Tizghadam, Hamidreza Azizi Faresani, Faranak Behnaz, Masih Ebrahimi Dehkordi* Pages 145-148
    Background

    Airway management is always of great concern for anesthesiologists especially in the prone position. Utilizing an Armored endotracheal tube is considered to be safe in such patients.

    Cases Report:

    In this current case, we introduce a patient undergoing a wake-up test during spinal surgery who suffered from hypoventilation at the end of surgery. His condition improved only after extubation. After extubation, we confronted a herniated cuff that was not deflated, although we tried twice. We concluded that the patient's respiratory effort against obstructed airway produced a negative pressure, which made the cuff herniated, resulting in airway obstruction. Moreover, the patient biting the tube during the wake-up test damaged the tube and cuff deflation pathway.

    Conclusion

    It is important to consider cuff herniation as a cause of obstruction when other possible etiologies are ruled out.

    Keywords: Endotracheal tube, Airway obstruction, Wake up test, Cuff herniation
  • Laleh Eslamian, Maasoumeh Saleh*, Behnaz Nouri Pages 149-151
    Background

    Postpartum infections, also known as puerperal fever, are bacterial infections of the female reproductive tract following childbirth or miscarriage. Symptoms usually include a fever greater than 38.0°C (100.4°F), chills, lower abdominal pain, and possibly bad-smelling vaginal discharge. It usually occurs after the first 24 hours and within the first ten days following delivery, but life-threatening infection may occur within hours of delivery.

    Cases Report:

    We reported a case of severe parametritis in the first 24 hours of delivery. Cesarean section is a risk factor for postpartum infections, and response to treatment is lower than vaginal delivery. Our case was a severe pelvic infection after vaginal delivery, and its risk factor was prolonged rupture of the membrane. Therefore, rapid diagnosis and treatment are necessary to prevent abscess formation and sepsis.

    Conclusion

    In this case, rapid treatment with broad-spectrum antibiotics prevented abscess formation, sepsis, and hysterectomy.

    Keywords: Postpartum infections, Puerperal fever, Severe parametritis, Postpartum endometritis
  • Maryam Fazeli, Saman Pazira, Behzad Pourhossein, Azadeh Rasooli, Nastaran Ansari, Farid Azizi Jalilian Pages 152-155
    Background

    Ruthin's coronavirus disease 2019 (COVID-19) diagnosis is based on the positive result of real-time polymerase chain reaction (PCR) from the nasal and oropharyngeal swab. However, chest CT scans can play an important role in diagnosing patients with COVID-19.

    Cases Report:

    In this study, we reported a 44 years old female with a mild form of the COVID-19 who showed a positive result for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA for 44 days after symptom onset. The suspected case was detected using real-time PCR. After two weeks of hospitalization, the patient was discharged, but her molecular tests were performed twice after one month and 44 days, and they remained positive for SARS-CoV-2 RNA.

    Conclusion

    In theory, if the patient becomes re-infected or the virus reacts, these individuals may serve as a transmission source. So far, the only way to screen for possible reinfection has been by using PCR on separate specimens.

    Keywords: COVID-19, Real-time polymerase chain reaction, Patient outcome assessment