نشریه زراعت و فناوری زعفران
سال نهم شماره 3 (پیاپی 33، پاییز 1400)

In order to investigate the effect of compensatory behavior of planting density, corm weight and planting depth on vegetative characteristics and yield of saffron stigma (Crocus. Sativus L.), a factorial split plot experiment with a randomized complete block design with three replications in the research center and Agricultural education and natural resources of Khorasan Razavi (Gonabad Research Station) was conducted in the cropping years 2018-2020. In this study, planting density treatment (in four levels of 60, 90, 120 and 150 corms per square meter) in the main plot and factorial planting depth (in two levels of 15±2 and 25±2 cm from the soil surface) and corm weight (in two Levels 4± 1 and 8 ± 1 gr) were located in the subplot. The results showed that increasing the density from 60 to 150 corms per square meter caused a significant increase in flower number, flower fresh weight and stigma dry weight, number of plants, number of leaves per plant, average length of a leaf and total leaf length per square meter. Accordingly, increasing the weight of coriander from 4 to 8 gr significantly increases flower number, flower fresh weight, stigma dry weight, number of plants, number of leaves per plant, average length of a leaf, total leaf length per square meter and total Leaf length per plant in the first and second years and reduced flower number, flower fresh weight and stigma dry weight in the third year. The results also showed that increasing the planting depth from 15 to 25 cm caused a significant decrease in all studied traits of saffron. The effect of dual and triple interactions on the studied traits was mainly significant. Overall, the results showed that the use of larger mother corms has a relative priority only in the early years and the importance of its effect decreases with increasing farm age. Based on this, it seems that it is possible to have a different strateg and with a combination of less depth and especially more density to compensate for the effect of corm weight and achieved more overall performance.

Carbon footprint (CF) is the total amount of greenhouse gas emissions per unit of farmlands. Since the used inputs have an important role in greenhouse gas emissions, CF as an ecological indicator have been extensively applied for assessing the environmental externalities in agroecosystems. This study was conducted to estimate the CF and carbon efficiency (CE) of saffron production systems in North Khorasan, Razavi Khorasan and South Khorasan provinces. Also, life cycle assessment analysis is calculated for quantifying the impact of saffron farming activity on the environment. Studied indices were global warming potential (GWP), acidification potential )AP) and eutrophication potential in terrestrial (UPT) and aquatic (UPA) sub-categories, N2Odirect, N2Oindirect, N2O emissions affected as volatilization and leaching, carbon inputs (Ci), carbon outputs (Co), CF and CE. The results revealed that the lowest GWP for saffron production systems was related to south Khorasan with 339.43 kg CO2 equiv./ one kg flower yield. The minimum environmental index (EcoX) was recorded for south Khorasan (0.039 EcoX/ one kg flower yield). N2O emissions in South Khorasan, Razavi Khorasan and North Khorasan provinces were estimated with 95974.51, 199674.4 and 344723 kg N2O per one ha, respectively. The largest N2O emissions affected as leaching and volatilization were calculated for North Khorasan province (with 1.21 and 24.23 kg N2O per one ha, respectively). The maximum Ci and Co were related to North Khorasan province with 117986.52 and 15135.56 kg C per one ha, respectively. The largest CF and CE were computed for North Khorasan and south Khorasan provinces with 7.8 and 0.18, respectively. It concluded that adoption on conservation and reduced tillages, N2- fixing pulses, cover crops and green manures in rotations with saffron and increased nitrogen use efficiency as ecological approaches can optimize the system performance while reducing environmental externalities and the carbon footprint of the crop cultivation. So, with relevant agro-environmental policies in saffron production systems along with the adoption of improved agronomical practices increasing flower yield with no cost the environment can be achieved effectively, efficiently and economically.

In the present study, the conventional solvent extraction (CSE) was compared with ultrasound-assisted extraction (UAE) and subcritical water extraction (SWE) methods for the extraction of bioactive constituents from Crocus sativus corms. The response surface methodology (RSM) based on a central composite face-centred design (CCD) was used to optimize the extraction parameters. The optimum conditions of extraction were estimated to be ethanol 80%, 48˚C and 60 min in CSE, ethanol 80%, 37˚C, 45 min, and 37 kHz in the UAE, and 180˚C and 22 min in SWE. The subcritical water extraction showed the maximum extraction yield, and the highest value of total phenolic compounds (807.6 mg gallic acid equivalent in 100 g saffron corm) and total flavonoid (12.2 mg Quercetin equivalent in 100 g saffron corm) were determined in the SWE extract. The phenolic and flavonoid content of the SWE extract is 8 and 12 times higher than the other two methods, respectively. Also, CSE and SWE extracts showed the least and the most antioxidant activities, respectively. The antibacterial activity of the optimized extracts was evaluated by the microdilution method. The results showed the minimum bactericidal concentrations of 300 and 600 mg mL-1 against Staphylococcus aureus and Escherichia coli bacteria, respectively.

Saffron (Crocus sativus L) is an important medicinal plant cultivated in different parts of Iran especially Razavi and South Khorasan provinces and because of its high price considered as one of the important Iranian products for export. Saffron extract is rich source of phyto chemicals with antioxidant properties and used in traditional and modern medicine. Crocin is one of the natural carotenoid in saffron extract which recently used in pharmaceutical formulations for treatment of depression. In regard to significance of phyto chemicals and bioactive compounds in saffron extract and specially crocin, in this study, a convenient and practical solvent extraction method has been examined for extraction of crocin and bioactive compounds from saffron. For this purpose, at different ethanol-water volume ratios 0-100, 25-75, 50-50, 75-25 and 100-0, the ultrasonic extraction of bioactive compounds and crocin has been achieved. UV-Vis spectra at max of 445 nm was used for absorbance measurements and least square curve fitting applied for determination of crocin concentrations in extracted samples. The results obtained at different ethanol-water volume ratios and the stability of the solutions studied after three days. Ethanol-water with composition of 50:50 (v/v) was selected as the optimum ratio for the extraction of crocin from saffron.

Given the importance of saffron corms in improving crop yields, it is necessary to supply standard corms that meet the standards required to ensure high yields, especially in the early years of cultivation. Given that currently, there is no official market for this product (standard corm), this study attempted to use the conditional valuation method to measure the maximum willingness to pay of saffron farmers for this non-marketed product, while investigate the factors affecting the selection of this type of corms. The study population consisted of 150 saffron growers in Torbat Heydariyeh and Zaveh area of Razavi Khorasan province. Using Multiple Logit Model to determine factors affecting the selection of one of the certified saffron corms (Type A, corm weighing 5-10 g and up to 60% flowering in the first year, Type B corm weighing 10-15 g and 80% flowering in the first year, Type C corms weighing 15-20 g and up to 90% flowering in the first year, Type D corms weighing more than 20 g and more than 90% flowering in the first year), it was found that increase in education, area, pre-crop treatment and having a lateral occupation significantly increases the probability of selecting corms type A. In contrast, increasing of age and onion weight decreases the likelihood of selection of these corms. Likewise, in selection of B and C corms, education, area of cultivation, lateral occupation, age and pre-cultivation treatment were found to be significant and effective. After estimating the parameters of the Multi Logit model, the expected mean value of willingness to pay (WTP) was set at 82500 Rials, which is higher than the current average price of corms available on the market indicating that saffron growers are willing to pay more for these certified corms.

This study aimed to improve the level of quality classification of saffron and increase farmers' profits by creating a hypothetical market to investigate the factors affecting selection and the willingness of farmers to pay for saffron quality testing using contingent valuation method and open-ended question. data were collected by a questionnaire from a random sample of 170 saffron producers in Khorasan Razavi province. Two-stage Heckman model was used to identify the factors influencing choice and estimation of willingness to pay. data analysis showed that about 48 percent of farmers are willing to pay an additional amount for a quality test, of which 29 percent showed willingness to pay more than 900,000 rials per kilogram of flower. The results of the model's estimation also showed that age, education, land ownership and history of saffron quality testing in the past have a positive and significant effect on the likelihood of choosing and the amount of willingness to pay extra for quality testing. Accordingly, the use of proper and comprehensive training, holding public organizations and promotional activities can play an effective role in encouraging farmers to conduct quality tests.Key words: Saffron, Willingness to pay, Contingent valuation, Two-stage Hackman model.This study aimed to improve the level of quality classification of saffron and increase farmers' profits by creating a hypothetical market to investigate the factors affecting selection and the willingness of farmers to pay for saffron quality testing using contingent valuation method and open-ended question. data were collected by a questionnaire from a random sample of 170 saffron producers in Khorasan Razavi province. Two-stage Heckman model was used to identify the factors influencing choice and estimation of willingness to pay. data analysis showed that about 48 percent of farmers are willing to pay an additional amount for a quality test, of which 29 percent showed willingness to pay more than 900,000 rials per kilogram of flower. The results of the model's estimation also showed that age, education, land ownership and history of saffron quality testing in the past have a positive and significant effect on the likelihood of choosing and the amount of willingness to pay extra for quality testing. Accordingly, the use of proper and comprehensive training, holding public organizations and promotional activities can play an effective role in encouraging farmers to conduct quality tests.Key words: Saffron, Willingness to pay, Contingent valuation, Two-stage Hackman model.