مجله علوم باغبانی ایران
سال پنجاه و دوم شماره 4 (زمستان 1400)

Environmental compatibility assessment is the most important needs of Rosa damascena genotypes as one of the most important aromatic and medicinal species in the country. The present project was conducted with the aim of evaluating the flower and essential oil yield of five superior genotypes for five years (2011-2015) under two climatic conditions of cold semi-arid (Saqez) and semi-humid (Marivan) in Kurdistan as split plot with three replications. The results showed significant difference for flower and essential oil yield between locations (L), years (Y) and genotypes (G), and their interactions. The total mean of essential oil ratio was 0.02 % and flower and essential oil yield were 757.2 kg/ha and 188.9 g/ha. The flower and essential oil yield of Saqez was more than Marivan, but essential oil ratio was more in Marivan, then it seems that flower yield to be higher in the cooler areas but essential oil ratio to be higher in warmer areas. In general, the genotypes of KR2, IS8 and IS4 are recommended for planting in Kurdistan and similar regions. Regarding to the fluctuation and high flower yield, KR2 genotype is recommended for the purpose of flower production, but Isfahan group of genotypes, such as IS8 and IS4 for essential oil purpose. With consideration of the combined yields of flowers and essential oils, the genotypes of IS8, IS4 and KR2 are recommented for cold and semi-arid areas such as Saqez, and the genotypes of IS4 and KR2 for the semi-humid regions similar to Marivan.

To investigate the effect of intercropping corn/cucumber and okra/cucumber on growth indices and yield of cucumber, an experiment was carried out in a randomized complete block design with three replications, during 2013-2014 cropping year, in Ravansar region in Kermanshah province. Treatments included: intercropping corn/cucumber (cucumber %100, cucumber %25 + corn %75, cucumber %50 + corn %50, cucumber %75 + corn %25) and okra/cucumber (cucumber %100, cucumber%25 + okra %75, cucumber %50 + okra %50, cucumber %75 + okra %25). Among the different treatments, cucumber %25 + corn %75 treatment had the highest plant length (276 cm), maximum number of fruit per plant (44.33) and the highest fruit yield (41344 kg/ha). Maximum number of lateral stem (6 per plant) was obtained from cucumber %25 + okra %75 treatment. Results indicated that land equivalent ratio (LER) was greater than 1.0, when both intercropping corn/cucumber and okra/cucumber crops were planted. The LER of corn/cucumber intercropping was 1.24, when cucumber %75 + corn %25 was planted. Economic efficiency of intercropping was affected by different intercropping combinations. The highest economic efficiency was obtained from cucumber %25 + corn %75 treatment. Moreover, in the cucumber %25 + okra %75 combination, economic efficiency was significantly less than cucumber pure crop.

Due to the fact that the common substrate for production most of the edible-pharmaceutical mushrooms is the sawdust of trees. But the replacement of other lignocellulosic compounds with enrichment with chemical supplements is of great importance in the production of fruiting body of mushroom. In this study, which was conducted at Zabol University in 1997, the substrates were prepared based on various agricultural wastes. The aim of this study was to investigate composition and non-composition substrates, and to investigate some vegetative, reproductive, nutritional and medicinal properties of iranian isolate Hericium erinaceus (Lion's mane). Treatments included sugarcane bagasse + poplar sawdust (50 to 50), sugarcane bagasse + rice bran (70 to 30), poplar sawdust + rice bran (65 to 35), poplar sawdust, sugarcane bagasse, wheat straw, sugarcane bagasse + date palm sawdust (60 to 40), poplar sawdust + date palm sawdust (65 to 35), date palm sawdust and wheat bran + date palm sawdust (40 to 60) that enriched with chemical supplements of ammonium nitrate (80 ug. g d.m), manganese sulfate (20 ug.kg d.m) and nano-manganese oxide (20 ug.kg.dm). The results showed that in the composition substrate of date palm leaf and wheat bran, the vegetative growth of the mushroom was completed in the shortest time. Mushrooms produced from composition substrate sugarcane bagasse with poplar sawdust had the highest amount of total polysaccharides of fruit body as well as highest yield of fruit body of mushroom (fresh weight) belongs to composition substrate sugarcane bagasse with poplar sawdust enriched with nano-manganese oxide supplement. The highest values of antioxidant capacity (73.45%) were attributed to mushrooms produced on composition substrates sugarcane bagasse with poplar sawdust enriched with nano-manganese oxide supplement. Also, the highest amounts of calcium (23.07mg/100g dry matter of mushroom) in the fruit body belong to the composition substrates of poplar sawdust with rice bran enriched with ammonium nitrate (65 to 35).

Nepeta binaludensis Jamzad is an endemic and rare perennial plant that grows in a limited area in Binaloud Mountain in northeast of Iran. The aerial parts of 20 populations of N. binaludensis, were collected during their flowering stage and seven morphological traits and essential oil yield were evaluated. Results showed significant differences in all of traits between populations (p<0.01). The highest and lowest aerial fresh weight were obtained in Zoshk1 and Grineh populations with average values of 378.2 and 58.2 g/plant, respectivel. The highest and lowest essential oil yield were obtained in populations of Darood and Friezy1 with average values of 4.91 and 1.2%, respectively. The results of correlation analysis showed that plant height was positively correlated with stem diameter, aerial fresh and dry weights. Climatic factors such as rainfall, altitude and slope percentage were positively correlated with morphological traits and essential oil yield. Morphological traits and essential oil yield were positively correlated with climatic factors, such as rainfall, altitude and slope percentage and negatively correlated with temperature of the habitat, indicating that mean of mentioned traits were increased by increasing altitude and decreasing of temperature. Cluster analysis using Ward method, the studied populations were splited into two groups. The populations of cluster 1 (seven populations adapt to high altitude and cold) had higher mean values for morphological traits, i.e. plant height, aerial fresh and dry weights. Results indicate the presence of high diversity among populations of N. binaludensis that could be used for domestication and breeding improved varieties of this species.

To investigate the effect of foliar application of calcium carbonate nanoparticles (CCN) and indirectly increasing carbon dioxide concentration on salt stress modulation of rose 'Dolce Vita., a soilless culture experiment was conducted as a randomized complete blocks design with three replications, in which the pots were filled with a mixture of cocopeat and perlite. The first factor was salinity at two levels (1.8 and 3.2 dS m-1 by adding NaCl to the nutrient solution) and the second factor was foliar spraying of CCN at four levels (0, 3, 6 and 9 g L-1 by using Lithovit fertilizer). Increasing salinity and the concentration of CCN had no effect on potassium and sodium contents of leaves and electrolyte leakage of leaf cells. The salinity of 3.2 dS m-1 reduced the relative humidity of leaf and increased the dry matter percentage and soluble sugar content in the plant leaves; however, had no significant effect on studied morphological characteristics. The application of CCN significantly increased the relative humidity of leaves in both saline and non-saline treatments and the mean of morphological traits (leaf area, flower diameter, stem length and flower yield). In addition, the application of 6 and 9 g L-1 CCN significantly increased the concentration of chlorophyll, carotenoid, and leaf soluble sugar; however, salinity had no effect on these characteristics. Results showed that foliar application of CCN, by affecting physiological and morphological traits and improving plant nutrition, modulates salinity stress and improves the growth conditions of Dolce Vita rose.

The use of treatments such as nanofertilizers and hormones in soilless culture are important factors influencing the quantity and quality of horticultural products. In order to investigate the interaction of nanopotassium (0, 1, 2 and 3 g/l) and sodium nitroprusside (0, 200, 400 and 600 μ mol/l) on strawberry fruit (cv. Camarosa) under hydroponic culture system, a factorially experiment based on completely randomized design was carried out. The results showed that all morphological characteristics including fruit number, fruit length and diameter and fruit weight were statistically significantly different from control samples. The highest number, length and weight of fruit were observed in the treatment of 400 μmol/l sodium nitroprusside and 1 g/l of nanopotassium. The results showed that all phytochemical properties and antioxidant and enzymatic activities were statistically significantly different from control at 1% level of probability. The highest amount of total phenol was observed in the combined treatment (2 g/l nanopotassium and 400 μmol/l sodium nitroprusside) and the highest PAL enzyme activity was related to the combined treatment (3 g/l nanopotassium and 600 μmol/l sodium nitroprusside). In general, the results of the present study showed that the treatment of nanopotassium and sodium nitroprusside can increase the quantity and quality of strawberry fruit and increase its marketability significantly.

This study was conducted to investigate the effect of nitrogen and iron elements on increasing the quantity and quality of the 6-old-years sweet lime trees budded on Mexican lime rootstock in Jahrom. The experiment was performed as factorial arrangement in the randomized complete blocks design with 9 treatments and three replications and each replicate having one sweet lime tree. The foliar application of urea and iron sulfate was done at concentrations of 0, 3 and 5 g/l, alone and their combined treatments, before flower induction of the trees, in October. Based on the results, different amounts of urea and its interaction with iron sulfate, had significant effect on all quantitative and qualitative traits of sweet lime fruit at p<0.05. Iron sulfate had significant effect (p<0.05) on pH, total acid, vitamin C, fruit weight, yield. The highest yield of single tree (203 kg), juice percentage (45.4) and vitamin C (44.26 mg/100 ml fruit juice) were obtained from the combined treatment of nitrogen 5 g/l and iron 3 g/l concentration.

This trial was conducted in order to evaluating nine apple rootstocks including MM106, M26, Ottawa3, Suporter4, M9, M7, P22 , MM111 and local rootstock "Gamialmasi" in different levels boron (0, 15, 30, 45 and 60 mg/kg soil). Results indicated that height rootstock, LAI, wet and dry weight leaf, chlorophyll index, WRC and vegetative growth rate reduced in comparison control as increased boron soil in all rootstocks. Vegetative growth ceased in all rootstocks, exception M26 and P22. Also, ion leakage and MDH increased as raised boron level in soil. Ascorbate peroxidase and super oxide dismutase leaf activity increased at first and then decreased with increasing boron levels in soil. Based on leaf analysis, leaf boron concentration in M26 and P22 was less than other rootstocks. Vegetative, physiological and biochemical assessments showed that M26 and P22 were more tolerate in relation other rootstocks.

The marshmallow is used in Iranian traditional medicine and the pharmaceutical industry for various ailments, including digestive problems, skin irritation and coughs. Zinc plays an important role in the metabolic activities of plants and can affect the quantity and quality of medicinal plants. Osmopriming of seeds could be helping to increase germination, coming out of the soil, establishment, and growth of plants in the field conditions. The experiment was carried out as factorial based on a randomized complete blocks design. The first factor was  seed osmopriming with polyethylene glycol 6000 at four levels (control, -3, -6 and -9 bar) and the second factor was zinc foliar spraying from zinc sulfate source at four levels (0, 1, 2 and 3 g. l-1). Osmopriming on dry weight of the flowers and mucilage yield and zinc foliar spraying on dry weight of flower, number of seeds per fruit and mucilage yield had a significant effect. Treatments had no significant effect on 1000-seed weight. The interaction of osmopriming and zinc foliar spraying in the traits of the number of flowers and fruits per plant, dry weight of fruit, seed yield and mucilage percentage was significant. Foliar spraying 2 g.l-1 of zinc increased the mucilage yield and the flower dry weight, 46% and 57% compared to the control, respectively. The highest mucilage percentage was obtained from control treatments. Generally, the highest yield of flowers and mucilage was obtained from the middle levels of treatments (2 g. l-1 zinc sulfate and -6 bar osmopriming).

Lemon grass has been cultured in Iran due to its economic value and applications in food and pharmaceutical industries. Limonene is on  of the important compound in lemon grass essential oil. In this study, different plant growth regulators were used for callus induction in lemon grass. Root, shoot and leaf explants wee cultured on MS medium containing 2, 4-D (1, 2 and 4 mg.l-1), BAP (0.1, 0.2 and 1 mg.l-1), hormonal combination of BAP + NAA (0.5+ 0.5 mg.l-1), 2, 4-D + BAP (1+ 1 mg.l-1) and BAP + NAA (2+ 1 mg.l-1 ). The experiment was conducted as a factorial experiment in a completely randomized design with three replications. The results showed that the best treatment for increasing callus size, fresh and dry weight was BAP + NAA (0.5+ 0.5 mg.l-1 ), from leaf explants. Biotic and abiotic elicitors including MJ (50, 100 µM), SA (50, 100 µM), Tio2 ( 0.5, 2 mg.l-1) and yeast extract (0.5, 2 mg.l-1) were used for increasing limonene production in lemon grass cell culture. Used elicitors had no significant effect on limonene production in cell culture.

In order to study the effect of vermicompost and biological nitrogen fertilizer on essential oil and quality of summer savory, an experiment was conducted at Firouzkuh in 2015. The experimental was as a factorial experiment based on randomized complete blocks design with three replications. The first factor was nitrogen biofertilizer at three levels (non-inoculation, seed inoculation with Nitroxin, and seed inoculation with Supernitroplus) and the second factor was vermicompost at four levels (0, 5, 10 and 15 t ha-1). The results showed that the highest essential oil content and alpha-terpinene obtained by 10 and 15 ton vermocompost per hectare, respectively, and the highest content of para-cymene, and gamma-terpinene were obtained by using 5 ton vermicompost per hectare. Supernitroplus had positive effects on the essential oil, alpha-terpinene and gamma-terpinene, while the maximum para-cymene obtained by using Nitroxine. The highest carvacrol content was obtained by using 10 ton vermicompost per hectare integrated with Supernitroplus and the maximum alpha thujene content was observed by using 5 ton vermicompost per hectare and non-using n-biofertilizers. The results showed that vermicompost and biological nitrogen fertilizer could have an effective role in sustainable production systems.

Spinach, due to high nutrition and economic value, highly regarded by the breeders. In this research, 40 populations belonging to three species of Spinacia oleracea, S. turkestanica, and S. tetrandra from most parts of Iran, along with a wild population from Afghanistan and two commercial cultivars Viroflay and Baby spinach, formed the studied germplasm. Thirty-three traits were classified under three groups of morphological, physiological, and biochemical. The results showed that the germplasm had a rich genetic structural diversity for the studied traits. The average coefficient variance of the studied traits was 29.8%. Oxalate accumulation showed a significant difference in the studied populations. The minimum oxalate accumulation was observed in the Karaj population of S. oleracea with 682 mg per 100 g FW. The maximum accumulation was found in the Bazangan population, the wild species of S. turkestanica, with 2902 mg per 100 g FW. The cluster analysis results put the two wild species populations in the same group and separated from the other ones. The coefficients of correlation were ranged from -0.83 to 0.99. In total, 43.1% out of all trait's interactions had a significant correlation (α = 0.01 or 0.05). The correlation results showed that the plant with a higher performance index had more freshness after harvesting. Also, the blistering was the only trait of among of all that had a significant negative correlation with oxalate accumulation.

In order to investigate the effects of kaolin foliar application on pomological and yield characteristics of some olive cultivars in Kermanshah province, a factorial experiment was conducted in a randomized complete block design with three replications. The first factor was olive cultivars at six levels (Amphisis, Seville, Roughani, Manzanillo, Konservolia and Zard) and the second factor was kaolin at three levels (zero as control (foliar application with water) and 2 and 4%). Two stages of complete foliar application of olive trees with kaolin, in mid-July and August (due to warm and dry weather during this period and accumulation oil in olive at the same time) was carried out. Recording of reproductive traits including fruit and oil yield, fruit weight, fruit dimensions, fresh and dry weight of pulp, percentage of dry matter and moisture content of fruit, fresh and dry weight of pit, pit dimensions and percentage of oil in wet and dry matter was recorded in different treatments. The results showed that the response of the studied olive cultivars to foliar application of different levels of kaolin was different. The highest fruit yield per hectare and fruit weight were obtained in Konservolia cultivar and 4% kaolin foliar application and the lowest yield and fruit weight were observed in Roughani cultivar. In all studied cultivars, kaolin foliar application increased fruit weight and yield per hectare. Therefore, by using kaolin foliar application in two stages of fruit growth, it is possible to improve the pomological and yield characteristics of different olive cultivars in warm and semi-warm regions of the country.

In this study, the quantitative and qualitative characteristics of the olive oil of three promising genotypes (T2, T7 and T18) and two cultivars (Zard and Koroneiki) from Tarom olive collection in Zanjan province were evaluated during two crop years (2017-2018). The results showed that T18 genotype with 70.96% and 69.58% oil in flesh dry matter had the highest amount of oil during 2017 and 2018, respectively, compared to other cultivars and genotypes. Also T18 genotype had the highest percentage of oleic acid and total unsaturated fatty acids with a double bonds. The oleic acid content of T18 genotype in 2017 and 2018 was 80.3% and 79.8%, respectively. In addition, the amount of palmitic acid in T18 genotype (9.5% and 10.3% in 2017 and 2018, respectively) was also lower than other cultivars and genotypes. T7 genotype had higher linoleic acid (14.2 and 18.67% in 2017 and 2018, respectively) and lower oleic acid (66.8 and 60.5% in 2017 and 2018, respectively). Based on principal component analysis (first four components), the first component was mostly associated with the traits of positive effect on the olive oil quality such as oleic acid and unsaturated fatty acids and increasing the oil percentage. In general, according to the results of this experiment, T18 genotype was superior to other cultivars and genotypes in terms of quantity and quality of oil and can be included in the cultivar introduction program.

To evaluate the effect of different levels of nitrogen and phosphorous on some physiological and morphological traits of golden rod (Solidago canadensis L.) a split plot experiment carried out based on randomized complete block design with three replications in Research Farm of Shahrekord University. Nitrogen fertilizer at four levels of (0, 50, 100 and 150 kg/ha) as main factor and phosphorous fertilizer at three levels (0, 50 and 100 kg/ha) were used as subsidiary factor. Results showed that different levels of nitrogen, phosphorous and their interactions had a significant effect on growth indices such as shoot fresh and dry matter, leaf area and flower flavonoid content. Also, nitrogen and phosphorous application showed a significant effect on flowering time and flowering duration. Increasing in nitrogen and phosphorous levels, caused early flowering and increased flowering period in treatments as the best results observed in 150 kg/ha accompanied with 100 kg/ha of nitrogen. Generally, in order to obtain the maximum growth indices in same conditions, application of nitrogen in 150 kg/ha with phosphorous in 100 kg/ha can be suggested for golden rod cultivation as an ornamental and medicinal plant.

Creating new efficient technologies such as doubled haploids is a fast solution for producing inbred lines. In this study, using the microspore embryogenesis method, doubled haploid (inbred) pepper lines were produced. The Topcross test was performed using 32 doubled-haploid lines produced and the topcross parent of California Wonder to examine the general combining ability of the lines. Hybrids derived from crosses line × tester in 2018- 2019 were planted. In order to evaluate the general combining ability of the lines, days to germination, days to flowering, days to fruit set, fresh fruit weight, dry fruit weight, fruit length, fruit width, thickness of fruit diameter, number of seeds, average yield per plant, number of fruits per plant and plant height were measured and analyzed. Comparison of means by Duncan test showed that top-cross progeny of DH55, DH64, DH57, DH53, DH217, DH202, and DH90 doubled haploid lines respectively with average of 8.58, 7.22, 7.18, 5.99, 5.93, 5.80 and 5.69 kg per plant had the highest fruit yield, among them the highest amount was related to the Topcross offset from DH55× California Wonder with an average of 8.58 kg per plant. The heritability of the evaluated traits was over 70%, among them the number of days to germination and the average fruit yield per plant were the highest. The above-mentioned lines also had the highest positive and significant general combining ability for the average yield per plant. Therefore, these 7 lines can be used as superior parent lines in future breeding programs.

This study was conducted to investigate the effect of different concentrations of agar and carbohydrate on micro-propagation of dwarf cherry rootstock (PHL-C) under in-vitro conditions in 2017 year. This experiment was conducted as factorial in a completely randomized design with six replications and three plantlets in each replication. The first factor was agar in three levels (0.5, 0.6, 0.7%) and the second factor was carbohydrate in six levels (sucrose and sugar each in 3 levels of 20, 30, 40 g / l) and evaluated in proliferation, rooting and adaptation steps. The single node was used for propagation The results showed that there was no significant difference between different agar concentrations in terms of proliferation index, rooting percentage, plantlet quality, plantlet fresh weight and survival percentage, but in other measured traits, agar with concentration of 0.7 % had the best results. Except in the traits plantlet height, leaf number and plantlet fresh weight which the sucrose had a more positive effect, in other traits there was no significant difference between sugar and sucrose. Due to the fact that in the essential traits of in vitro propagation of PHL-C, sugar has a better effect, it is suggested to use table sugar or a combination of sugar and sucrose to reduce the cost of producing the culture medium, especially in commercial production.

To evaluate the effect of Trichoderma application under drought stress conditions on biochemical properties and photosynthetic pigments of basil, an experiment was conducted in a completely randomized design with three replications. The studied treatments included 3 levels of irrigation (50, 75, and 100 Percent of field capacity) as the first factor and inoculation with Trichoderma (concentration 108) and non-inoculation with Trichoderma as the second factor. The results showed that the highest fresh and dry weight of leaves was observed in the treatment of irrigation area of 100 Percent of field capacity without the use of Trichoderma (control). Wet weight (17.69 Percent) and dry stem (17.27 Percent), chlorophyll a (0.66 Percent), chlorophyll b (0.64 Percent), total chlorophyll (0.66 Percent), proline(52/25 Percent) and the relative content of leaf water (1.37 Percent) in the irrigation level treatment of 100 Percent of field capacity along with the application of Trichoderma increased compared to the control and the amount of electrolyte leakage (7.5 Percent) in the treatment of 100 Percent of field capacity Decreased with the use of Trichoderma compared to the control.. The amount of carotenoids (31.74 Percent) and antioxidant activity (17.22 Percent) in the irrigation surface treatment of 50 Percent of field capacity with the use of Trichoderma were observed more than the control. According to the results, the application of Trichoderma harzianum increased proline, soluble carbohydrates, photosynthetic pigments, and antioxidant activity of the plant under different irrigation levels.

The selection of elite parents and hybrids could be used for the genetic improvement of quantitative and qualitative traits in breeding programs of vegetables. In order to genetic evaluation of quantitative and qualitative traits of tomato lines, half-diallel crosses were performed among eight lines C.JPS3, S.2274, H.1370, K.2274, S.L, C20, Primoga and A13012. The obtained hybrids along with parents were planted in a randomized complete block design with three replications. Traits including fruit shape index, no. of locules, fruit firmness index, brix, titrable acidity, pH of fruit juice, no. of inflorescence per plant, no. of flowers per inflorescence, no. of fruits per plant, average fruit weight and yield were measured. Parent K.2274 had the highest values of general combining ability (GCA) for the yield, number of fruits per plant and fruit firmness. The highest specific combining ability (SCA) was observed in C20xC.JPS3 and A13012xK.2274 cross for the average fruit weight, respectively. Among parents and hybrids, parent H.1370 and Primoga × H.1370 hybrid had the highest yield. The highest relative heterosis percentage for no. of fruits per plant, average fruit weight and yield were observed in A13012 × Primoga, C20 × C.JPS3, and Primoga × H.1370 crosses, respectively. Role of over-dominance and dominance effects were more than the additive effect in controlling of yield, no. of fruit per plant, titrable acidity, and brix. However, the additive effect was effective in the control of average fruit weight.

The purpose of this study was to investigate the effect of biochar and mycorrhizal fungi on growth, plant biomass and some nutrients of two parsley cultivar. A factorial experiment based on completely randomized design with three replications was conducted. The first factor was parsley variety at two levels (crispum and neapolitanum). Application of biochar at three levels (0, three and six percent of soil weight) was used as the second factor. While, application of Arbuscular mycorrhizal fungi in two levels (mycorrhiza and non mycorrhiza) was considered as the third factor. The results showed that the highest fresh and dry weights of aerial parts (210.218 and 64.8783 gr, respectively) was related to crispum cultivar with applications of mycorrhizal and biochar 3%. The highest fresh and dry weights of root (70.74 and 18.68 gr, respectively) and the highest colonization rate (96%) were observated in neapolitanum cultivar using mycorrhizal and biochar 3%. The highest amount of sodium, potassium, nitrogen and copper was obtained by application of biochar 6%. Mycorrhizal treatmeant also increased the concentration of magnesium, iron, copper and calcium in both neapolitanum and crispum cultivars. Finally, the highest amount of phosphorus (8918.678 ppm) was observed in neapolitanum cultivar with application of mycorrhizal and biochar 6%, while the highest concentration of manganese (50.7296 ppm) was obtained in crispum cultivar using mycorrhizal and biochar 6%. In general, in this study, application of biochar led to better colonization of parsley root with mycorrhizal fungus. Therefore, it can be concluded that the combined use of biochar and mycorrhizal fungi provides better conditions for achieving sustainable agricultural goals and increasing the growth of parsley.