نشریه اکوفیتو شیمی گیاهان دارویی
سال دهم شماره 3 (پیاپی 39، پاییز 1401)

Dorema ammoniacum D. Don. is a perennial herbs from Apiaceae family that grows wild in central and eastern regions of Iran such as Yazd, Isfahan, Kerman, Semnan and Khorasan provinces, and its local names are Kandal, Vasha or Oshagh and Koma-kandal. There is some evidence in Iranian traditional medicine about the anticonvulsant and anti-inflammatory properties of D. ammoniacum gum resin exuding from its root and stem. In this study, roots, stems, leaves, flowers and gums of D. ammoniacum were collected in the spring 2018 from Birjand in South Khorasan province. After drying the samples, the essential oils were extracted by hydro distillation and their content and compositions were investigated with gas chromatography techniques. The highest and lowest yield of essential oil in D. ammoniacum was obtained for gum and roots with a value of 1.0% and 0.2%, respectively. Based on the analysis of essential oils, 73 compounds were identified in organs and gum. In roots, stems, leaves, flowers and gum essential oils 91.3 %, 97.15 %, 91.8 %, 88.4 % and 89.4 % of the total components were identified, respectively. (Z)-sabinene hydrate, (2E, 6E)-farnesol, elemicin and n-hexacosane were common compounds among all essential oils. In addition, a series of monocyclic bisabolene compounds such as α-bisabolol, Z-α-bisabolene, β-bisabolene, ar-curcumene, ar-dihydro turmerone in different organs of the D. ammoniacum essential oils. The percentage of bisabolene compounds identified in different D. ammoniacum essential oils was 35.5% for root, 3.4% for stem, 1.4% for leaf, 14.5% for flower and 8.5% for gum essential oil. The most identified compounds in the D. ammoniacum essential oil were sesquiterpene compounds.

The aim of this study was to investigate the effect of habitat on the quantity and quality of essential oil of Teucrium stocksianum Boiss. Accordingly, the branch of this species at the time of flowering (August 2017 and 2018) were collected from 4 regions of Hormozgan province (northern side of Tang Zagh mountain at 1100 meters of altitude; Abmah mountain at 850 meters of altitude; Tang Zagh mountain at 1400 m and Enveh mountain at 2000 m) . Essential oils were extracted by hydrodistillation method ( Clevenger apparatus) and were analyzed by using GC and GC/MS. The results showed that with decreasing altitude, the amount of essential oil reduced, so that the lowest and highest amounts of essential oil belonged to the samples of Abmah mountain at 850 m and Enveh mountain at 2000 m, respectively. Chemical analysis of T. stocksianum essential oils showed that the common compounds in the essential oils of this species were elmol (2.4 to 8.5%), caryophylene oxide (3.0 to 7.1%) and β-eudsmol (2.2 to 12.1%). Compounds such as α-pinene and myrcene, the most volatile compounds found in essential oils, were not observed in lower-altitude samples, which may be related to high air temperatures in plant geographical location. Also, significant amounts of compounds such as valerianol, polgon, limonene and epi-alpha-cadinol were observed in some samples, which shows the effect of the ecological and climatic conditions on the quality of plant essential oil.

Drought stress is a major environmental concern worldwide that limits crop growth on a large scale, and the use of biofertilizers and amino acid spraying is important to overcome this issue to improve crop production. For this purpose, a two-year study was conducted on Salvia officinalis L. in the form of split-split plots in a randomized complete block design with four replications in a farm located in Kangavar city of Kermanshah province.   The treatments of this experiment were Irrigation at two levels [50 and 100% of field capacity (F.C.)] which was placed in the main plots. The sub-factor included biofertilizer with three levels (control, mycorrhiza and cow manure) in the subplots and the sub-factor included foliar application of L-phenylalanine at four levels [no foliar application (negative control), solution spraying with distilled water (positive control), spraying with concentrations of 25% and 50% L-phenylalanine]. The highest amount and yield of essential oil were related to the interaction effects of irrigation treatments of 100% field capacity + cattle manure + 50% of L-phenylalanine with amounts of 3.5% and 24.41 kg/ha, respectively. In addition, the interaction effects of irrigation treatments were 100% field capacity + mycorrhiza + 50% L-phenylalanine with values 3.6% and 23.65 kg/ha, respectively.

The aim of this study was to Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in a complete randomized design with three replications. R. persica L. was collected from Shirvan city located in North Khorasan province in the late summer of 2019 . The methanol, petroleum ether, ethyl acetate and water extracts were prepared by maceration method and were analyzed by using the liquid chromatography-mass spectrometer system, it was determined that the methanolic extract had the highest amounts of rosmarinic acid compounds. Antimicrobial effects of different extracts were investigated by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Antimicrobial assays against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens, Escherichia coli, Candida albicans, Aspergillus niger showed that in all treatments, gram-negative bacteria were more resistance in compare to gram positive bacteria. In all extracts, the most sensitive strain was S. aureus with MIC and MBC of 10.89±0.13 and 19.34±0.33 μg/ml in the methanolic extract, respectively. Methanolic extracts of aerial parts had significantly stronger antimicrobial effects, respectively. Based on the results of cytotoxicity of different extracts on MCF-7, DU-145, PC3, A2780, C26, U-87-MG, MDA-MB-231, and Hela, it was found that Methanolic extract had the most cytotoxic effects on all cell lines studied. The lowest effects of cytotoxicity were related to the aqueous extract of aerial parts. In all treatments and cell lines, the IC50 was less than 400 μg/ml. The biochemical and biological activities of R. persia may be related to its phenolic compounds, so further studies is recommended.

Blackberry (Morus alba L. Var. Nigra) has phytochemical compounds and antioxidant activity. In the present study, the fruits of four blackberry genotypes were harvested from two West and East Azerbaijan in mid-July 2017 . Phytochemical indices were evaluated based on the content of total phenol (folate sizing method), total anthocyanin (absorption difference method at different pHs) and antioxidant activity (DPPH). To separate, identify and determine the amount of phenolic acids and sugars a high-performance liquid chromatography device (HPLC) was used. Data analysis was performed by Duncan's multiple range test at the probability level of 1%. According to the variance analysis results, the measured traits were significant at the level of 1% and a high diversity was observed among the measured traits. The highest amount of antioxidants was 78.04 %, total anthocyanin and total phenol were 6.24 mg/ml and 925.98 mg/100 g fresh weight equivalent to gallic acid, respectively. In the current study, 9 polyphenolic compounds were identified in the fruit extract by HPLC. The main constituents included chlorogenic acid (60.060), coumaric acid (8.807) and caffeic acid (3.657) microgram per gram. In addition, in extracts, cinnamic acid (0.355), rosemary acid (0.055), gallic acid (2.59), rutin (0.833), apagenin (2.700), quercetin (1.861) microgram per gram were identified and also two sugars of fructose (5.84) and glucose (6.31) g per 100 g of fresh weight were reported. The results showed that in all genotypes, glucose was higher than fructose. The first genotype with the highest amount of antioxidant capacity, fructose, total phenol, caffeic acid, chlorogenic acid, quercetin and apagenin was introduced as the superior breed. The findings of this study is useful for understanding the diversity and efforts to select berries for breeding as well as for the food industry in selecting cultivars with high nutritional properties.

In this study, to investigate the phytochemical changes of essential oil and growth performance of Thymus daenensis Celak. affected by light and salicylic acid, an experiment was conducted as a split plot in a completely randomized block design with three replications. The tested factors included salicylic acid foliar spraying at three levels (0-0.2 M) and two light levels (50-100%). Special nets were used to create 50% shading. The amount of light reduction compared to the control treatment was measured by a lux meter. Foliar spraying was done before flowering. Plant essential oil was obtained from flowering aerial branches by Clevenger apparatus and analyzed by GC-MS. The highest yield of flowering branches and height were from 0.1 M salicylic acid (33.35 kg/ha and 24.33 cm, respectively) and full light treatments (32.50 kg/ha and 22.47cm respectively). The highest amount of chlorophyll a (5.2 mg/g), chlorophyll b (1.98 mg/g) and the number of lateral branches (180/35) due to the application of 0.1 M salicylic acid × 50% light treatment significantly increased compared to the control treatment. The amount of essential oil decreased with increasing light intensity and the use of salicylic acid improved this attribute. So that with full light intensity it reached the lowest level of 1.95% and the application of 0.1 M salicylic acid increased the percentage of essential oil (3.1) compared to the control treatment. The most components of thyme essential oil with the use of 0.1 M salicylic acid× 50% light treatment include carvacrol (4.4%), paracetamol (14.6%), beta-caryophylline (5.95%). Also, the highest amount of thymol was related to 0.1 M salicylic acid treatment (59.66%) and full light (57.8%). In general, the results showed that the use of salicylic acid has a positive effect on improving the qualitative and quantitative characteristics of the essential oil components of thyme. (5.95%). Also, the highest amount of thymol was related to 0.1 M salicylic acid (59.66%) and full light (57.8%). In general, the results showed that the application of salicylic acid had a positive effect on improving the qualitative and quantitative characteristics of the essential oil components of thyme.

Tanacetum parthenium (L.) Schultz. with a worldwide reputation as af ever reducer, pain reliever, antiseptic and anti-inflammatory has similar therapeutic uses in the natural habitats of Golestan province. In this research, to investigate the phytochemical and antioxidant properties of this plant, the plant leaves were collected from two different forest habitats of Golestan province, 990 meters (Ziyarat) and Kohustan Daraz Nu (2200 meters), located in the southwest of the province at full flowering stage at the end of July 2018. Essential oils were evaluated and analyzed by using the water distillation (Celvenger apparatus) and GC/MS methods, respectively. Extraction was obtained by using maceration method, the total phenol and flavonoids as well as evaluation of antioxidant performance of the extract samples were measured using DPPH and spectrophotometry methods, respectively. Based on the results more than 30 compounds were in both samples, which accounted for 97.3% the camphor (43.12-54.8 %), camphene (6.9-10.04 %), bornyl acetate (6.06-3.1 %), 1-8-cineol and p-cymene (3.1-3.36 %) that were the most important components of essential oil in both habitats. With the increase in altitude in the habitat of 2200 meters the  total flavonoid and total phenol increased up to 148.2 ± 0.7 mg GAE /gr and 218.7 ± 218.7 mg GAE /gr, respectively, while the highest antioxidant activity was also up to 89.5 ± 1.1% in DPPH free radicals. The findings shows that secondary metabolites was increased in high altitude habitats and this issue has been documented in the scientific documentation of medicinal uses of this plant in high altitudes as anti-inflammatory, pain reliever and treatment of infections.

With the increasing resistance of bacteria to antibiotics and their side effects, the use of medicinal plants in the treatment of bacterial infections is increasing. The aim of this study was to evaluate the phytochemical, antioxidant, and antibacterial properties of different concentrations of methanolic extract of Physalis alkekngi. For this purpose, the calyx and fruit of the P. alkekengi L. were collected from an area near the city of Tonekabon in the summer of 2019 from a height of 120 meters and the antimicrobial activity of the extracts on pathogenic bacteria was determined by disk diffusion methods, MIC, and MBC. Total phenol and flavonoid contents were measured by folin-ciocalteu reagent and aluminum chloride colorimetric method, respectively. In addition, total anthocyanin was measured by spectrophotometry as well as antioxidant activity of extract using DPPH method. Based on the results, the highest total phenol content at a concentration 50 mg-1 ml of calyx extract was 32.92±1.738 mgEGA g-1 DW. Also, the content of flavonoid and anthocyanin compounds in the calyx extract was more than that of the fruit extract. Based on the results of antioxidant activity also showed  that the highest DPPH radical inhibitory effect were obtained at concentrations of 100 and 150 mg/ml of calyx extract 51.43% and 51.58%, respectively. In addition, calyx and fruit extracts showed significant inhibitory activity against the studied bacteria as the highest inhibitory effect on Staphylococcus aureus was obtained at a concentration of 1000 mg -1ml of calyx and fruit extracts. The results of this study indicated that the calyx and fruit extracts of P. alkekengi are rich in antioxidant compounds, especially phenolic compounds, and the antimicrobial activity of the extracts showed a significant positive correlation with total phenol content. Therefore, the antimicrobial properties of the calyx and fruit extracts may be attributed to their phenolic compounds and these extracts can be recommended for medicinal applications.