Estimation of degradability and fermentation characteristics of some feedstuffs using in vitro and in situ techniques

The purpose of this study was protein fractionation and determination of degradability and fermentative characteristics using nylon bag and gas production methods. In this experiment, 2 ruminaly fistulated Gizel mature wether were used. Data were analyzed using SAS software as compeletly randomized design. After 48 h of incubation, barley grain and alfalfa hay had greatest and lowest gas production volume among test feeds (335.84 and 244.82 ml/g DM, respectively). The gas production potential amounts of alfalfa hay, corn and barley grains were obtained 254.16, 298.81 and 374.27 ml/g DM (P<0.05), respectively. Barley grain had greatest estimated metabolizable energy concentration among the feedstuffs. Alfalfa hay had the biggest true protein and buffer soluble protein fractions among feedstuffs. Alfalfa hay and corn had biggest and smallest ADIN among feedstuffs, respectively. In situ DM disappearance of corn grain at 24 and 48 h of incubation was higher than the other feedstuffs. Alfalfa hay had the lowest in situ dry matter disappearance value within the feedstuffs at the most incubation times. Barley grain and alfalfa hay had the highest and lowest CP a value (slowly degradable fraction) within the feedstuffs (16.77% and 14.16%, respectively). Potentially degradable CP fraction (b) and its disappearance rate for barely were more than the other feedstuffs. Alfalfa hay and corn grain had biggest and smallest metabolizable protein among feedstuffs, respectively. Among the test feeds barley grain and alfalfa hay had the biggest metabolizable energy and metabolizable protein, respectively.