Survey and Comparison of Immunization Scale of The Recombinant Proteins of Attachment Subunit of Tetanus and Botulinom(A) Toxins

The only presented vaccine against tetanospasmin (the main factor of the fatal disease tetanus) is its toxoid which creates a 10-year period immunization. Ho-wever، the botulinum toxoid (the main factor of the fetal disease botulism) only creates a 2-year period immunization. The-se two toxins have 36% homology and are falling into one family but are different in immunological memory، the scale of hom-oral immunity response and probably the scale of antibody production. The goal of this study was to compare the immunization and antibody titer of fragment C of both tetanus toxin (THc) and botulinom type A (BONT/A-Hc). Expression of the recombinant proteins، THc and BONT/A-Hc، was accomplished by using of the tran-sgenic host، E. coli Bl21 DE3 and the pET28a vectors which contained the two genes in an optimum condition. Both the mentioned proteins were derived and puri-fied from the solution phase of cell extract and were evaluated on SDS-PAGE gel. Finally، the antibody titer containing in the serum of immunized animals with each of the aforementioned proteins، was inv-estig-ated and compared by immunoassay test. The result of SDS-PAGE gel evaluation showed a proper expression and purification of both the recombinant prot-eins، THc and BONT/A-Hc، in solution phase of cell extract. The immunoassay res-ults of serum showed that the antibody titer against the recombinant protein of THc was more than those for the recombinant protein of BONT/A-Hc. Discussion & It is conceived that the significant differences in the antib-ody titer can be related to the longevity of memory cells. However، the result needs more studies.