Study Effect of Fenugreek Seed Extract on HPG Axis and Spermatogenesis in Male NMRI Mouse

Population control is an important problem in the society that performs by chemical contraceptive drugs. Today, using the safer methods with lesser side effects and the usability of them for men were concerned more than before.In this research, the effects of fenugreek seed were studied on the spermatogenesis. It's hopeful; this study could present a new drug in population control that use by men.In this study, the mature male mice, NMRI strain, 3 month old (30±3 g) were used. Animals were divided into the four experimental groups and the one control (8 mice in each group). Experimental groups were obtained 70, 140, 280 and 560 Ml/kg dosage of alcohol extracts of fenugreek seed during 16 days. In this period, control group was obtained physiological serum by intraperitoneal injection. Then in 17th day, animal's blood was taken for hormone assays of measuring the testosterone, LH and FSH hormones. After that, animals were morphometrically and histologically studied and testis weight and volume, epididym weight and the number of sperms in epididyms. Statistical results were analyzed by ANOVA, tukey test (p<0.05). There were no significant differences between testis weight and volume and epididym weight between experimental and control groups. There were no significant differences in testosterone and LH levels between experimental and control groups, but there was significant difference in FSH level between them.Significantly decrease was observed in the number of sperm in epididym between experimental and control groups. Obvious decreasing of sperms in the result of reduction of FSH level, confirm that the fenugreek seed extract effects on decreasing of male reproductive activities.