The effect of acute exercise on total antioxidant capacity and hydrogen peroxide in male Wistar rats

The aim of this study was to determine the amount of total antioxidant capacity and hydrogen peroxide changes after an acute exercise in Wistar rats. 14 male Wistar rats were randomly divided into two groups as control and acute exercise groups. Acute exercise intensity was set to 16 to 26 m/min for one hour، or until exhaustion on a treadmill running. Blood samples were collected 1 hour after exercise. Total antioxidant capacity and hydrogen peroxide were detected by FRAP and FOX-1 methods respectively. When the normal distribution of the data was revealed by Kolmogorov-Smirnov test، it is applied independent t-test at significance level of p<0. 05. Results showed that TAC (p= 0. 002) and H2O2 (p= 0. 003) significantly increased in the acute exercise group as compared with control group. Although the level of hydrogen peroxide increased in response to the acute exercise، improved antioxidant capacity may be indicator of response of rats antioxidant system to the performed acute exercise.