The molecular epidemiology evaluation and antibiotic resistance patterns of Salmonella isolated in food outbreaks in Yazd province by culture and PCR methods

Salmonella is one of the most causes of food contamination in food chain thought the world. Increasing antibiotic resistance is also pressing health threats facing the world today, and makes Salmonella as one of the most important pathogens of gastroenteritis in food outbreaks. The aim of this study was to investigate, the molecular epidemiology evaluation and antibiotic resistance patterns of Salmonella isolated in food outbreaks in Yazd province by culture and PCR This was a descriptive cross-sectional study and, 141diarrhea stool swab from 39 food outbreaks were studied in Yazd providence over a period of year. The samples were inoculated into selenite F broth for enrichment and then cultured into Hektoen enteric agar or xylose lysine dextrose Agar for the growth .The suspected colonies were chosen for the identification by biochemical and serologic tests .The identification was confirmed by PCR method. The antibiotic susceptibility test was performed by disk diffusion method. Out of 141 samples 11 (7.8%) were isolated as Salmonella. All the isolated serogroup was type D. Laboratory conducted antibiotic resistance testing showed that entirely 100% of isolates Salmonella were susceptible to ciprofloxacin and imipenem whereas (73.07%) were resistant to Nalidixic acid . All isolates were confirmed by PCR. Salmonella commonly known as a pathogen that caused gastroenteritis in food out breaks. Awareness of the common serogroup and antibiotic resistance patterns would help the treatment.