Evaluation of hypericin in Hypericum perforatum L. (Case study: Golestan National Park and Ramian)

Hypericin is the most important active compound of Hypericum perforatum. Thus, the knowledge of changes in hypericin level in the nature helps to recognize the requirement of H. perforatum under agricultural conditions. Two sites of Golestan National Park in the west and Ramian the East of Golestan Province was selected according to the distribution of the in the province. For sampling, first, four altitude classes of 300, 600, 900 and 1200 m were considered in the range that the plant was observed at the location. The samples were collected from the 20-25 cm terminal part of flowering shoots, at the full-flowering stage. Methanolic extract of the samples were obtained by cold maceration method and the hypercin content was determined by HPLC in the sample of different sampling sites. Data analysis was performed using SPSS software. The results showed that at both studied sites, hyperacin content increased along with increase in altitude. There was a significant (P