Evaluation of native phytobiotic effects on immune response of fish and shellfish

Probiotic bacteria play an important role in the development of optimal fish culture. This study was carried out to evaluate the effect of Bacillus subtilis probiotic isolated from common carp, on fatty acid composition of fish fillet with an average weight (54/56 ±1 g). Control group fish with common diet and experimental treatments with supplemented diets were fed in two levels of concentration 103 (treatment 1) and 106 (treatment 2) CFU/ ml (cells per ml). The experiment in a completely randomized design with three replications was carried out for 8 weeks. Feed rate was based on 2% of body weight and 2 times at a day. Determination of length and weight were carried out every 14 days. The results of the fatty acid profile showed no significant differences in the amounts of SFA, MUFA, Omega-3, Omega-6 among all with (P>0.05). PUFA and PUFA levels in treatments decreased significantly as compared the control and treatment 2. The
highest amounts of EPA and DHA, were observed in the treatment 1 and the control. The results of this study showed that probiotic bacillus can be affected on fatty acid composition of common carp.