Differentiation of Human Adipose-Derived Stem Cells toward Neural Cells Using Their Conditioned Medium
Author(s):
Abstract:
Background
Human adipose-derived stem cells (hADSCs) are population of mesenchymal stem cells isolated from adipose tissue. Multipotent hADSCs can be differentiated into different cell lineages and they have ability to secrete variety of cytokines such as growth factors. These factors have neuroprotective effects and lead to survival and neural differentiation. The aim of this study was to investigate the effects of concentrated hADSCs conditioned medium (hADSCs-CM) on neural differentiation.Methods
The adipose tissue samples were obtained from young donors after signing the consent. The stem cells were isolated and cultured through several subcultures. In order to neural differentiation, the cells from 3rd to 5th passages were treated with hADSCs-CM for 21 days. The cells survival was assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and neural differentiation of cells was confirmed via immunocytochemistry.Findings: MTT assay indicated that there was significant difference (P
Conclusion
This study reveals that concentrated hADSCs-CM improves the survival and neural differentiation of hADSCs. Our results suggest that hADSCs-CM may pave the way for non-cellular treatment of neurodegenerative diseases.Keywords:
Language:
Persian
Published:
Journal Of Isfahan Medical School, Volume:35 Issue: 435, 2017
Pages:
726 to 730
magiran.com/p1723691
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!