Influence of Different Concentrations of Butylated Hydroxy Anisole on Quantity and Quality Parameters of Mahabadi goat Semen Following the Freeze- Thaw Process

Present study was conducted to evaluate the supplementation of different levels of antioxidant Butylated Hydroxyanisole (BHA) on the improvement cryopreservation of Mahabadi goat sperm. Four healthy Mahabadi goats (3-4 years) were used to collect semen samples by artificial vagina, once a week for five weeks during non-breeding season. Good quality samples were pooled and divided into five equal aliquots supplemented with extender containing different concentrations of BHA (0, 1, 2 and 4 mM). Sperm parameters, including motility and progressive motility, viability, membrane integrity and total abnormality were assessed. According to the results, BHA at the concentration of 4 mM significantly improved sperm post-thaw viability, membrane integrity, total and progressive motility (P0.05). Furthermore, abnormality percentage was not significant among different levels of BHA and control groups (P>0.05). Current study indicated that inclusion of BHA in extender may be an approach to improve post thaw goat sperm quality.