Optimization of extraction of Ferula persica plant extract using ultrasound and supercritical fluid methods and investigation antioxidant activity of its extract

The most simulating factor introduced in this researches is efforts to limit the use of synthetic antioxidant in food this is dangerous for human health. The present research was carried out aimed to optimize the extraction of phenolic and tocopherols compounds of the Ferula persica plant with the help of ultrasound and supercritical fluids new technology and study of anti-oxidative properties of the extracts. Therefore, the amount of phenolic, tocopherol compounds and antioxidant power of the extracts using Folin - Ciocalteu method, α- tocopherol, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and β- carotene - linoleic acid has been investigated, and compared with a synthetic antioxidant, Tertbutylhydroquinone (TBHQ). The results showed that there was no significant difference between the amounts of phenol (1495.81 ppm) and tocopherols (58.6 ppm) extracted by supercritical method compared to the amount of phenol (1422.52 ppm) and tocopherols (56.66 ppm) extracted by ultrasound statistically. free radical scavenging percentage DPPH (72.97%), and being colorless in βcarotene system (63.4%) at concentration of 2500 ppm of extract extracted using supercritical fluid had the highest value, and there was no significant difference between it and the synthetic antioxidant TBHQ, but there was observed significant differences compared with the ultrasound method at the same concentration. The results indicate that, the Ferula persica plant extract has good anti-oxidant property as well as extraction method and concentration of the extract is effective on antioxidant power.