Effect of two irrigation methods and two types of fertilizers on phenolic compounds and antioxidant activity of Rosa damascene Mill. extract

Phenolic compounds (flavonoids, tannins, and anthocyanins) are the most important natural antioxidants. Rosa damascene Mill. is one of the most natural medicine, which has been used in Iranian ancient and were cultivated traditionally in different parts of Iran. The present study was conducted to investigate the quantitative and qualitative properties of phenolic compounds and antioxidant activity of Rosa damascene Mill. under the influence of two types of fertilizers (livestock manure and fertilizer), as well as two irrigation methods (flushing and dripping) in Kashan university (2015). The total phenolic, flavonoidal, alkaloids, tannin contents and the antioxidant activity were measured by spectrophotometer and DPPH methods, respectively, then all data were analyzed by using SPSS software version 19 and analysis of variance. The results of the phytochemical test confirmed the presence of secondary compounds such as tannin, anthocyanin and flavonoids, and the absence of alkaloids in the extract of this species. Also, the total flavonoid content in the sample used for chemical fertilizer was slightly higher than the other samples. The Livestock manure and flood irrigation treatments showed the highest and lowest phenolic compounds respectively and drip irrigation treatment had the most antioxidant properties. The results of this research were showed that the irrigation and nutrition can be controlled the production, quantity and quality of secondary compounds in plants.