Diagnosis of Chlamydia Trachomatis Infection in Symptomatic Women Using Polymerase Chain Reaction and Amplifying the MOMP Gene

Chlamydia trachomatis is the most common sexually transmitted bacteria among curable sexually transmitted infections. This bacterium is associated with female genital tract infections while most infected people are asymptomatic. The aim of current study was to detect Chlamydia trachomatis in symptomatic females using polymerase chain reaction (PCR) and MOMP gene amplification.

The current investigation, as a cross-sectional study, was conducted on 200 symptomatic females who referred to MohebYas Hospital from June 2013 to June 2014. Samples were collected using Dacron swabs from the endocervical area, and then DNA was extracted and amplified by polymerase chain reaction. The data were analyzed using Chi-square test in SPSS software version 20.

The most common symptoms were the lower abdominal pain (73.5%), dysuria (70.5%) and vaginal discharge (67.5%); respectively. 16 (8%) patients infected with Chlamydia were diagnosed by Polymerase Chain Reaction. There were significant relationships between the Chlamydia infection with education degree and the antibiotic consumption (p

It is suggested to Chlamydia trachomatis screening in sexually active people to minimize the subsequent effects.