Association between B2 Allele Frequency in CETP Gene with Low level of HDL-C in Tehran

The object of the present study was to investigate the association between common CETP polymorphism, TaqI in intron 1, and high density lipoprotein levels in Tehran population. In order to examine the relationship between B2 allele and HDL-C level, 356 people with the lowest, medium and highest deciles of HDL cholesterol levels were selected out of 993 healthy subjects from TLGS. Factors known to influence HDL cholesterol level, such as smoking, body mass index and blood pressure were then studied. A segment of mentioned gene was amplified with PCR and then polymorphism was revealed with RFLP. The allele frequency distributions of the TaqI (intron1) polymorphism differed significantly between the groups with low(<28mg/dl) and high(>50mg/dl) HDL cholestrol. Homozygotes for the B1 allele had lower HDL-C levels(36±11mg/dl) than subjects carrying B2 allele(46±13mg/ml)(P<0.001). The percentile of B2B2 allele in high HDL-C groups was more than low HDL-C groups(17.3%, 5.5% respectively). In conclusion, it was found out that TaqI polymorphism at the CETP gene is correlated with HDL cholesterol levels.