Decreased Expression of PSMB9 Gene in Patients with Endometrial Carcinoma
Endometrial carcinoma is the second most common neoplasm in women, and understanding the genes involved in its occurrence can help in understanding the disease behavior and, ultimately, in treating it.
There were 35 samples of frozen endometrial carcinoma tissues and 24 samples of normal endometrium adjacent to the tumors. Cell total RNA was extracted, followed by DNaseI enzyme treatment. Using random hexamers cDNA was synthesized. We measured PSMB9 gene expression by RT-qPCR using exon junction-specific primers. The statistical analysis was performed using REST2009 software.
The observation of two ribosomal RNA bands showed that RNA was not broken during extraction. The appropriate concentration of extracted RNA was in the appropriate range for cDNA. Examination of the melting curve showed that specific amplification of the PSMB9 gene was performed. The difference in PSMB9 mRNA expression between endometrial carcinoma tissue and adjacent normal tissue was 0.266 units. This was considered significant according to a P of 0.008.
There was a decrease in PSMB9 gene expression in endometrial carcinoma tissues compared with normal endometrial tissues adjacent to the tumor. The fact that PSMB9 expression has decreased may indicate that the gene has tumor suppressor functions.
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