Experimental study of Various Methods of Heparin Administration in Prevention of Adhesion in Infectious and Noninfectious Peritonitis in Guinea Pigs

Abdominal surgery may lead to intra-abdominal adhesion. Therefore scientists suggest different methods to prevent this problem. Use of heparin, by its mechanism of coagulation pathway inhibition, is one of these methods. The aim of this study was the comparison of various methods of heparin administration in prevention of post-surgical abdominal adhesion. In our experimental guinea pig model, Fourty guinea pigs were divided into eight groups in randomized manner: A(1-4) and B(1-4). A1 and B1 were negative control groups and did not receive any medications. All guinea pigs were anesthetized, laparotomy was performed on each one and 1.5cm midline incision was given. Then peritoneal inflammation was induced in the exposed bowl and peritoneum. After that, experimental infection was induced with inoculation of 100 cfu of Escherichia coli in peritoneal cavity of B(1-4) group. Each guinea pig from A2 and B2 groups received 80mg/kg/day heparin subcutaneously for three days, A3 and B3 groups received one dose of 100IU heparin intraperitoneally and A4 and B4 received 80mg/kg/day heparin three days subcutaneously and one dose of 100 IU heparin intraperitoneally concomitantly. Then the incision was sutured. On the 21 th postoperative day, guinea pigs were euthanized and peritoneal adhesions and specific inflammatory and regeneration reactions were evaluated. The results were analyzed with Fischer's exact test in SAS(version 6, 12) software. Peritoneal adhesions and inflammation reactions in experimental groups had reduced significantly(P<0.05) in comparison with controls, but insignificant differences were seen between experimental groups(P>0.05). Results revealed that subcutaneous, intraperitoneal and concomitant administrations of heparin are effective in reduction of adhesion formation in infectious and non-infectious experimental peritonitis without significant differences. Therefore since the method of intraperitoneal administration is much easier and more convenient than other methods it is recommended.