Bacteriological Study of Acute Diarrhea in Children Less Than 10 Years Old and Serotyping of Isolated Strains

Being aware of the pathology and immunology of acute diarrhea in children can help to prevent and treat the disease. The aim of the present study was to isolate and determine sterotypes of bacterial agents causing acute diarrhea in children under Age 10. This study was descriptive cross sectional and was done during a two year period from Nov 1998 to Sep 2000. The statistical population was 544 children with the age range between 3months to 10 years in Hamadan and the country referred to clinics for stool culture. Salmonella and shigella species were isolated by culturing samples on selenite F and ss. agar. E.coli and yersinia species were isolated by culturing samples on EMB, PBS and CIN agar. Differentiated Biocemical tests and specific poly and monovalent antisera were used for identification of isolated serotypes. Data was analyaed using EP 16 software. From 544 examined samples 134 samples (24.6%) were postive for one type of pathogenic bacteria. The frequency for each pathogenic bacteria was as the following: Entropathogen E.coli (EPEC) 88 samples (65.8 %), Shigella 26 samples (19.5%), Salmonella 13 samples (9.5%). The results of serotyping showed that the most frequent E.coli serotype was Ecoli 055 (38.6) and the least frequent E.coli serotype was Ecoli 0119 (10.3%). The most frequent Salmonella serotypes were Paratifi B and tifi (3.8%) and the least frequent serotypes were paratifi A & C (0.77%). with respect ot the result of this study using modern techniques recognition and identification of bacterial agents causing childern diarrhea in diffenent geographical region is suggested.