In vitro comparison of oocyte maturation rate and postfertilization development of zygote in bovine and buffalo

In the present study, 73.1 and 50 percentages of bovine (n=104) and buffalo (n=60)cultured oocytes were matured, respectively (P<0.05). In the second trial, using frozen sperm, 68.2 and 19.35 percentages of the bovine (n=93) oocytes were fertilized and developed to cleavage and blastocyct, respectively. Using fresh isolated buffalo epidydimal sperms, 16 % of buffalo oocytes (n=50) were fertilized and developed to cleavage. There was no blastocyst in buffalo IVF trial. The results have shown a significant higher competence of bovine oocytes. Because of different sources of sperm, the results of IVF between bovine and buffalo may not be compared.