Comparative Study of Lotus corniculatus L. Hydroethanolic Extract and Phenytoin Ointment Effects on Rat Skin Wound Healing: Morphometrical and Histopathological Studies

This study is examination skin wound healing after using of lotus corniculatus L. aerial part extract in comparison with Phenytoin ointment in rat. After anesthesia of 80 Wistar male rats, a rectangular wound was produced at back each of which. Healing woud steps was studied in 8 treated groups: control (A), 1% Phenytoin cream (B), 10% extract ointment (C), 20% extract ointment (D), 40% extract ointment (E), 1% Phenytoin cream + 10% extract ointment (F), 1% Phenytoin cream +20% extract ointment (G), 1% Phenytoin + 40% extract ointment (H). Medication and measuring wounds were done using digital images analysis for 21 days. Samples were collected from wound healing sites for microscopy after 3, 7, 14 and 21 days. Wound healing was determined for all of treated groups. Data were analyzed using SPSS18. Healed wound area mean in E and H groups was lower than comparing to other groups (P<0.01). Also based on histopathological results, E and H groups showed more healing wound in comparison with other groups. According to anti-inflammation, anti-microbial and healing effects of Lotus corniculatus in comparison with 1% Phenytoin, it is believed that the plant compounds are more effective in full thick skin wound healing.