نشریه زراعت و فناوری زعفران
سال یازدهم شماره 4 (پیاپی 42، زمستان 1402)

The storage conditions of saffron mother corms during the summer dormancy period, including natural and pseudo-dormancy stages, can probably affect the vegetative growth and the production of replacement corms (RC), which has not received enough attention in previous scientific studies. This experiment was carried out as a complete randomized block design with seven treatments and three replicates during the 2019 growing season in Qaen, Iran. Experimental treatments were three types of corm storage inside the soil [corm planting on 5th June (A) with natural soil temperature as control, A + summer irrigation on 5th August, and A + mulch application obtained from wheat residues at the rate of 8 ton.ha-1)] and four types of corm storage outside the soil [corm storage at room (ambient temperature) for three months started from 5th June + corm planting on 5th September (B), corm storage in constant temperature (25 oC and relative humidity of ~40 %) in incubator for three months + B, three months corm storage at variable temperature in incubator (one month in 20, 25 and 20 oC) + B and corm storage in room temperature (up to 5th August) and incubator (up to 5th September) + B. The studied traits were the length, number, and weight of leaves, the number and weight of the total RC, the weight of the main RC, the average weight of RCs, and the number and yield of RCs in different weight groups. The results showed that mulch application caused the highest length and number of leaves (35 cm and 9 No per plant, respectively). Incubation of corms at variable temperatures led to a significant increase in the total weight of RC. In contrast,  incubation at constant temperature had most significant effect on the number of RC (2022 corms per m2). The highest and the lowest mean weights of RC were obtained in the mulch application (7.23 g) and incubation at constant temperature (2.22 g), respectively. Plant residue treatment (mulch) decreased the number of small RC (less than 6 g) and increased the number of RC in the weight groups of 9-12 and 12-15 g. Incubation of mother corms at variable temperatures led to an increase in the number and yield of RC weighing more than 15 g. Overall, the treatments of plant residue consumption and storage of mother corms at incubator with variable temperatures, in which 66% and 61% of the RC yield was in groups weighing more than 9 g, respectively, were the best experimental treatments in terms of improving the growth of RC.

Recognizing the potential role of seaweed in improving saffron yield and the utilization of micronutrient elements like Ca, Mg, Fe, and Mn to enhance stigma yield, an experiment was conducted. The study investigated the impact of foliar spraying of seaweed extract and selected nutritional elements on saffron's quantitative and qualitative traits of saffron.This research was conducted, as factorial based on a randomized complete block design (RCBD) with four replications during 2021-2022 in Neishabur, Iran. The first factor consisted of seaweed extract (0 and 2 L.ha-1) and the second factor consisted of foliar application of nutrients including no foliar application (as control), and the defender of some elements including iron (1.5 L.ha-1 containing Fe, N, and amino acids), zinc, boron (1 L.ha-1 containing B and amino acids), manganese (1.5 L.ha-1 containing Mn, Zn and S), magnesium and calcium (2 L.ha-1 containing Ca and N). The use of all nutrients compared to the control (no-nutrients) and the use of seaweed extract compared to no-seaweed application caused an increase in flower and stigma yields. Based on the interaction effects of the experimental factors, the highest number of flowers (68.8 No.m-2) was obtained in the combined application of manganese and seaweed extract, the highest flower yield (33.5 g.m-2) was gained with the combined application of seaweed and boron, and the highest dry stigma yield (0.48 g.m-2) was obtained from the simultaneous application of magnesium and seaweed extract, which were 63.8, 16.7 and 17.0%, more than the control treatment (no-seaweed & No-nutrients), respectively. Without nutrient application, the utilization of seaweed extract resulted in a notable rise in the Picrocrocin content of the stigma by 15.9%, Crocin by 9.5%, and Safranal by 10.6% compared to the absence of seaweed application.Combined consumption of calcium with seaweed increased the content of Picrocrocin and Crocin, while simultaneous application of other nutrients with seaweed did not improve these indices. The highest and the lowest content of Safranal (37.7 and 32.35 absorption at 330 nm) were obtained from magnesium + no-seaweed and control (no-seaweed & no-nutrients) treatments, respectively. Overall, although all of the studied elements combined with seaweed extract improved stigma yield in order to increase stigma yield, application of Mg in combination to seaweed recommended and to enhance saffron quality using seaweed suggested.

Fusarium rot disease is one of the most important fungal diseases of saffron corms worldwide, which can lead to a decrease in product yield and the quality of daughter corms produced. The objective of this study was to isolate and identify agents responsible for Fusarium corm rot in saffron, as well as to assess their pathogenicity. In order to determine the Fusarium species from saffron corms with rotting symptoms were sampled from the different fields in provinces of Hamadan, Golestan, Kerman, Kurdistan, Semnan, Kermanshah, Ardabil, East Azerbaijan, North Khorasan, Razavi Khorasan, Isfahan, and South Khorasan. After isolation and purification, fungal isolates were identified and confirmed based on morphological characteristics and species-specific primers. Also, the pathogenicity of the isolates was artificially tested in the greenhouse on saffron corms according to Koch’s principles. The results indicated that approximately 38.6% of the saffron corms were infected with fusarium rot disease in the 1 to 5% range. 33 isolates were identified based on morphological and molecular characteristics belonging to F. oxysporum (26 isolates, 78.8%) and F. solani sensu lato (7 isolates, 21.2%). The pathogenicity tests revealed that all Fusarium isolates were pathogenic, displaying varying levels of disease index.The result of the host range test of F. oxysporum on different plants revealed that all isolates caused wilting and yellowing of aerial organs, necrosis and rotting of underground organs on Gladiolus (Gladiolus communis L.), Irises (Iris germanica L.) and Crocus (Crocus vernus L.) and was called as F. oxysporum f. sp. gladioli. This is the first report on identification of F. oxysporum f. sp. gladioli from saffron fields in Malayer, Bijar, Kangavar, Parsabad, Marand, Natanz, Najafabad and f F. solani sensu lato from saffron fields in Azadshahr, Shirvan, Esfarayen, Kashmar, Najafabad, Boshruyeh on saffron corms. The findings of this research provide new insights into the health status of saffron corm against fusarium rot disease, which can be used for the revision of the national standard for saffron corms and determining suitable areas for certified corm production of saffron.

Saffron is known as the most expensive spice in the world due to its many medicinal properties. This plant has many secondary metabolites, the most important of which are crocin, picrocrocin, and safranal. Different drying and storage conditions have caused a decrease in the quality and effective ingredients of saffron stigma, which has reduced its value and credibility for export. This research aimed to explore methods for preserving the quality and microbial characteristics of dry saffron stigma during storage. Saffron stigmas were packed using three-layer packages (polyethylene-polyester-polyethylene) under three atmospheres: air, vacuum, and N2.The packages were then stored at room temperature for 80 days. Once every 20 days, the concentration of oxygen gases, carbon dioxide, physicochemical properties, total microbial load and sample scanning image were measured. The results showed that the best samples for maintaining the physicochemical characteristics of saffron stigma were packages containing nitrogen-rich atmosphere, so the amount of crocin and picrocrocin decreased by 0.1% and 0.3%, respectively, while the amount of safranal increased by 0.3%. Also, the total microbial load decreased by one logarithmic cycle compared to the first day of storage in packages containing a vacuum atmosphere. Electron microscope images also showed that the samples under nitrogen atmosphere, like the control sample, had cell swelling and bubble-shaped particles along with cell membrane rupture and cell collapse.

This study used the benefit-cost analysis and various financial indicators, such as Net Present Value, Benefit-Cost Ratio, Internal Rate of Return, and Payback Period, to determine the economic feasibility of saffron production under controlled conditions in Mazandaran province. Data for calculating these financial indicators were collected through field surveys, questionnaires, interviews with experts and specialists from the Agriculture Jihad Organization of Mazandaran province, and by obtaining fixed and variable costs as well as income from the establishment of controlled environment spaces for saffron production (spaces with areas of 200 square meters, 1000 square meters, and 5000 square meters) from agricultural service centers. The collected data were analyzed and evaluated using COMFAR software version 3.3, and the results, including financial indicators and sensitivity analysis under different scenarios, were presented. The findings suggest that at a discount rate of 18% and an inflation rate of 35.06%, establishing a saffron production unit on a 200-square-meter plot is not financially viable within a seven-years. (one year for construction and six years for operation). However, for plots with areas of 1000 and 5000 square meters, the internal rate of return is 36.85% and 53.99%, respectively, making them financially viable. According to the project ranking test, increasing the plot area for saffron production under controlled conditions leads to higher financial profitability. Furthermore, based on the sensitivity analysis results, it was identified that increasing product sales income has a more significant impact on project profitability compared to reducing fixed and variable costs. Positive steps such as increasing production, effective marketing, and improving product quality can enhance profitability.

As the world's most expensive agricultural and medicinal product, saffron holds a special place among Iran's industrial and export goods. Customers constantly seek the best products in terms of price, quality, and timely delivery. Thus, it is crucial and necessary to ensure quality control in the saffron production process in order to satisfy customers and enhance profitability. To thrive in the competitive market, greater attention should be given to the critical quality characteristics of saffron, namely crocin, picrocrocin, and safranal. Moreover, statistical process control, a method for monitoring processes to identify the underlying causes of specific changes and deviations, is essential. Control charts and process capability indicators serve as necessary tools in statistical process control. Therefore, this paper primarily focuses on the use of the exponentially weighted moving average control chart, which is effective for handling minor deviations, to monitor and control the saffron production process in a data set during the initial phase. The data sets pertain to the qualitative characteristics of saffron in the cities of Torbat Heydarieh, Zaveh, Roshtkhar, Bardeskan, and Kashmar. Following the examination of the quality aspects of the saffron production process and its sustainability, emphasis is placed on the control chart as the most crucial statistical process control tool. Efficiency indicators of the saffron production process are then calculated and analyzed using standards 259-1 and 259-2. Through this analysis, efforts can be made to reduce process variability and consequently compare and enhance the quality level of saffron produced in the investigated areas. The results showed that in premium cut string saffron, Torbat Heydarieh city has the best picrocrocin, Kashmar city saffron has the best safranal, and Rashtkhwar city saffron has the best crocin. Also, the best picrocrocin and saffron type 1 string saffron belong to the town of Torbat Heydarieh and the best safranal belongs to the town of Bardeskan. Utilizing these indicators in the saffron production process serves as a measure to evaluate the processes' ability to meet customer demands and comply with relevant industry standards.