بهزاد نیکبخت

Beef contamination usually occurs in the killing process. One of the intestinal bacterial pathogen that causes disease in human and animals everywhere is bacterial pathogen E. coli. One of the important serotypes of this bacterium is E. coli O157: H7. The aim of this study was to determine the rate of contamination of beef produced in Zahedan to E. coli by MPN Method and also O157:H7 strains isolated by PCR. During this study, conducted within six months from July 1396 to July 1397, 360 beef samples from cattle meat packages of Zahedan City, from different parts of the carcasses isolates of the heat-resistant E. coli in the laboratory was carried out using the MPN method. Seventy isolates were finally detected. The final test was performed using a specific primer as rfb to detect E. coli O157:H7 using a PCR reaction. Only one isolate was confirmed based on this serotype, which indicates the proper health status of the meat produced in Zahedan. In the present study, a molecular study of 2 isolates of heat-resistant Escherichia coli bacteria was done. In the study of rfb gene, only one isolate in terms of Escherichia coli serotype 7H: 157O was confirmed. With this study, the presence of this bacterium in beef samples is confirmed, which can indicate that the presence of this bacterium in other raw animal products in this province should also be investigated and tracked.

Astigmatism is a common complication of penetrating (PK) and Lamellar (LK) keratoplasty that may cause surgical failure due to visual disturbances. In this study, the efficacy of scar revision just at the surgical incision was evaluated. In this prospective interventional case-series study, scar revision was performed in nineteen post-PK or LK astigmatism patients. In all patients, three months after suture removal, refraction, keratometry and topography was performed. Using Mendez gage, 2 gaps of, 45 degrees for astigmatisms of less than 6 diopter, and 60 degrees for astigmatisms of more than 6 diopters was induced in steep meredians. Magnitude of refractive error and corneal keratometry before and at least 3 months after intervention were measured. Mean age of patients was 30 (19-81) years. The reason for PK was KCN in 15, post-traumatic corneal scar in 2, and pseudophakic bullous keratopathy in 2 cases. Only in two cases, micro-perforation and leakage happened that were managed by eye patching alone. No other significant complication was observed during follow up period. The mean pre-operative astigmatism was 8.1 ± 4.5 (3.00-24.00) D which decreased to 5.4 ± 3.7 (0.5-14.00) D post-operatively (p<0.05). Scar revisions just at the graft junction, induced statistically significant corrective effect in post-PK astigmatism and can be used as an appropriate method for treatment of theses patients.

Laryngeal obstruction due to vocal cord paralysis has been treated in many ways. The purpose of the present study is to evaluate the outcomes of posterior cordectomy by co2 laser and its effects on respiratory complications due to vocal cord paralysis. Patients & In this pre and post-operative clinical trial, we made use of a 2-4 watt co2 laser which was connected to a laryngeal microscope with an x400 lens. The applied method was slightly different from Dennis and Kashima’s usual technique in terms of the application of mithomycin C on the surgical site. Ten cases including 3 females and 7 males underwent posterior cordectomy by co2 laser. The mean age of the subjects was 34.1 years, ranging from 11 to 60. The duration of follow-up ranged from 5 to 24 months with a mean of 17 months. All the patients were suffering from severe dyspnea before the surgery and 8 of them had tracheotomy for their dyspnea before the surgery. The day after the surgery all the patients got decanulated. The changes of breathing, voice and swallowing conditions were analyzed pre and post-operatively using paired t-test. The mean FEV1/FVC ratio that was 67% preoperatively increased to 80% postoperatively(P<0.8). None of the cases encountered aspiration after the surgery. Four of the cases needed a second surgery due to granulation or fibrosis on the site of the previous operation. Ultimately, the surgery resulted in regular breathing in all the patients. This surgery can decrease respiratory complications caused by vocal cord paralysis.