فهرست مطالب حسین تیموری نقده

Micro RNAs are a class of small non-coding RNAs which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. The correlation of microRNA expression levels with the pathogenesis of some hematologic malignancies including acute myeloid leukemia is already known. The aim of this study was to investigate the expression of mir-150 and mir-155 in chronic myelogenous leukemia patients. miRNA expression level changes can be used for diagnosis, treatment and estimation of Minimal Residual Disease (MRD). In this descriptive study, the expression of miR-150 and miR-155 in 25 newly diagnosed CML patients in chronic phase and 25 healthy subjects as the control all in Payvand Medical Laboratory were examined by Stem-loop RT-PCR and Real Time PCR techniques. For data analysis, Pearson correlation analysis was used. miR-150 and miR-155 were down-regulated in CML patients rather than in healthy subjects (0.512 and 0.2552, respectively). There was no correlation between the laboratory findings and the miR expression level. But miR-155 was inversely associated with age (p ≤ 0.01). In conclusion, the decreasing expression of miR-150 and miR-155 in chronic myelogenous leukemia is indicative of their induced expression in suppressing proliferation of cells in this disease. Other studies are also required to elucidate the exact role of microRNA by identifying their target genes.

The recomwell Yesinia uses YOPs (Yesinia outer membrane proteins) produced by recombinant techniques that are highly specific for the genus Yersinia. The aim of this study is to determine the prevalence of Yesinia Yop-specific IgA antibodies in 492 healthy blood donors and make comparison between seropositivity and the presence of y.enterocolitica infection in blood donors. In the descriptive study, sera from 492 healthy Iranian blood donors were studied for Yesinia Yop-specific IgA antibodies by two different techniques, enzyme immunoassay (EIA) and western blot to determine the prevalence of Yersinia antibody. Secondly, we cultured the whole blood of Yesinia seropositive donors which has been stored in 1-6 ◦ C for 35 days. Thirdly, we compared the rate of seropositivity with the results of donors’ blood cultures. The prevalence rates of Yesinia Yop-specific IgA antibodies in Tehran healthy blood donors were 12.5% and 8.5% by EIA and Western Blot, respectively. The blood cultures of all seropositive donors were negative. Although the prevalence rate of Yesinia Yop-specific IgA antibodies in Iranian healthy blood donors was high there was no correlation whatsoever between seropositivity and donors’ bacteremia.

Hemoglobin screening methods before blood donation need to be not only simple، rapid and inexpensive but also sensitive for detection of both low and high hemoglobin levels to prevent false rejection of potential donors. The aim of this study was to evaluate the validity of two methods of hemoglobinometry in blood donors.

In this cross sectional study، 198 blood donors were randomly selected. The hemoglobin levels of two blood samples taken by means of a single finger stick were determined by Hemocue 201+ and Hemocontrol and hemoglobin level of a venous blood sample was measured by Cobas hematology analyzer as standard method. Venous hemoglobin range of 12. 5-17. 9 g/dl was regarded as inclusion criteria for blood donation. The sensitivity، specificity and correlation between reference method and each of the hemoglobinometers were assessed. Data analysis was performed by means of correlation tests، regression analysis and paired t-test.

Mean hemoglobin levels measured by Cobas، Hemocue and Hemocontrol were 15. 9±1. 68، 16. 28±1. 91 and 16. 30±196 g/dl، respectively. Hemoglobin levels measured by hemoglobinometers were higher than that measured by the standard reference. This difference was not significant in low hemoglobin levels، contrary to high hemoglobin levels. Each of the two methods showed significant correlation with standard method when hemoglobin levels were lower than normal limit. Increased hemoglobin levels led to decreased correlation coefficient which was not significant with hemoglobin levels higher than 18g/dl.

Our study results showed that these heomoglobinometers were not valid for screening high hemoglobin levels in blood donors and a large number of potential donors would be falsely rejected. Further studies to find valid methods are recommended.

Platelet concentrate (PC)، in the different times from collection until transfusion، undergo a considerable time period without agitation. The aim of this study was to compare the continuously agitated PCs in vitro properties، with non agitated PCs for 6 hours، just after termination of interruption in day3 of storage in Iranian blood transfusion organization (IBTO)، Tehran.

20 PCs were obtained from platelets pooled and divided into two groups. The first group designated as reference group with continuously agitating PCs; the second group or test group stored at stationary condition for 6 hrs under the same environment in the incubator. Day three was selected for evaluation، because PCs are transported to hospitals on the beginning of day three، the time that the agitation is interrupted. In-vitro variables، including pH، swirling، Ristocetin-induced aggregation، LDH (viability)، PF4 release and P- selectin expression (activation markers) were measured.

The mean level of pH in agitated and non agitated groups were 7. 16±0. 08 and 7. 22±0. 16، respectively (p=0. 3). Ristocetin-induced aggregation، LDH and swirling studies showed no significant differences between two groups، but 6h PCs showed less PFA release (p=0. 015) and P-selectin expression (p=0. 006) which was the important achievement.

According to findings of this study، especially for pH and swirling، 6hrs non agitated PC’s at 22-24 С has good quality compared with routine PCs.

Blood bank refrigerators play the main role in blood safety and appropriate blood production. Confirmation of their functions to maintain temperature stability with a PM program is compulsory. The main objective of this study is to identify the level of maintenance and regional climate effects on blood bank refrigerators in different blood centers. In order to study the level of maintenance and possible regional climate effects on each blood bank refrigerator، a survey on 85 local refrigerators in seven regional blood centers was carried out. In this survey، along with the amount of temperature leakage from the body and glass insulators، the quality of ribbons used was also evaluated. The results obtained from this study showed the degree of temperature leakage on bodies of the fridges، metal doors، and glasses to be varied due to different factors like room humidity and temperature changes، air flow around refrigerators، and spaces between refrigerators and other appliances or wall. This study contributes to a higher knowledge level of local manufacturers on how better to produce blood bank refrigerators to be compatible with different climate conditions of Iran.

Incorrect storage of blood and blood components within inappropriate temperature range not only nullifies the treatment effect but also brings about adverse effects. For cold chain system to be implemented, hospital blood banks should be well equipped, well trained staff should be involved, and standard operating procedures should be in place. Materials and Methods In this descriptive study, 50 hospital with permanent blood bank supervisors were selected to participate in the study in Mazandaran and Gilan Province. All cold chain equipment in hospital blood banks were directly observed; all observations were then made on the formulated questionnaires. The data were analyzed with Pearson and Spearman’s rank test by SPSS 16. Results Out of the 50 hospital blood banks under study, 17 (34%) lacked standard criteria. This in part could be attributed to the lack of blood bank special freezers although they were all equipped with special refrigerators. The variables of age, gender, work experience, and education degree of the staff had no significant correlation with blood bank criteria. The cold chain process is necessary to be followed for blood safety to be ensured. Although equipment deficiency is a main factor for blood banks not to be standard, regular personnel training and continuous supervision would be helpful for this trend to be enhanced.

Background and ObjectivesThere is growing evidence indicating that growth factors derived from platelets can be used in wound healing. This study aimed to investigate whether old platelets can be used as the main material for preparation of platelet gel and as substitute for FBS and FCS in cell culture medium. Materials and MethodsIn this exprimental study, platelets were prepared from voluntary blood donors by centrifugation. To prove the hypothesis that the platelet gel and the growth factor derived from expired platelets are able to propagate different cells, platelet derived factors were prepared from both new and expired platelet-rich plasma. The concentration of platelet-derived growth factors was measured by ELISA and cell proliferation was measured by MTT assay. ResultsThe results showed the high quality of platelet gel obtained from old platelets. Our results also revealed that old platelets released growth factors similar to those released by new platelets. The growth factors derived from old and new platelets had the same proliferation effects on MSC, CHO, and Fibroblast cell lines. ConclusionsOld platelets released the same growth factors that new platelets did; this showed that old platelets as valuable constituents of blood are cost effective to be used.

Background and ObjectivesAcute blood complications form the most frequent and important reactions among the blood transfusion adverse effects; acute hemolytic reaction, bacterial contamination, and transfusion related acute lung injury (TRALI) are the most frequent causes of death following blood transfusion. Materials and MethodsIn this descriptive prospective study, 2633 patients hospitalized in surgical and non-surgical departments of 11 hospitals having received blood transfusion were evaluated for acute blood transfusion reactions. ResultsAmong these patients, 20 (0.8%) encountered acute blood transfusion reactions. Out of this group of patients, eight experienced febrile non-hemolytic transfusion reactions, nine had allergic reactions, one developed blood group incompatibility, and two disseminated intravascular coagulation (DIC).ConclusionsThis study indicates that the most prevalent blood transfusion reactions were fever and allergy (0.3%). This reported rate (0.5-6%) is lower than the global rate. The rate of blood group incompatibility in this study was 0.03%, showing a higher prevalence than the global rate (0.004%).