فهرست مطالب راجنش کومار شارما

In mammalian females, the uterine tissue is highly responsive to steroid hormones and their antagonists.

In the present study, topographical, histoarchitectural, and gene expression changes in goat endometrium treated with estradiol, progesterone, and mifepristone for 24 h were investigated, in vitro.

Scanning electron microscopy (SEM) was used for surface topographical analysis; WGA and DBA lectins were used for histochemical analysis; and qRT-PCR was done for the quantification of mRNA levels of MKI67 (marker of proliferation Ki67), ESR1 (estrogen receptor), PGR (progesterone receptor), CASP3 (caspase 3), and PDGFR-β (platelet derived growth factor receptor-β).

Few topographical alterations were observed in endometrial glands and the presence of scattered mucoid granules. A significant decline in WGA staining was reported only in the progesterone group. However, DBA binding was highest in the progesterone group and lowest in the mifepristone group. The expression of MKI67 gene declined to 79% in the mifepristone group, while in the estradiol and progesterone groups it elevated to 153% and 41%, respectively, than control; a similar trend was observed for PDGFR-β. The mRNA abundance for ESR1 declined to 59% in the progesterone group and 10% in the mifepristone group. However, a 100% increase occurred in the estradiol group. PGR expression followed the same trend as that of ESR1. CASP3 declined in the estradiol (50%) and progesterone (37%) group, but it showed a 67% increase in the mifepristone group.

We concluded that the caprine uterus undergoes dramatic alteration in structure and functions in response to different kinds of steroidal environments.

The goat is a multi-purpose animal producing meat, milk, hide, fiber and manure, and hence plays a significant role in providing supplementary income and livelihood to the huge number of asset-poor ranchers and landless workers of rural India.

The present study is aimed to investigate the in vitro effects of prostaglandins E2 (PGE2) and F2α (PGF2α) on ovarian granulosa cells of a goat.

The healthy, slightly atretic and atretic antral follicles of goats with diameter ranging from 3-8 mm were cultured with PGE2 and PGF2α (1.0 μg/ml) along with control for 24 h. Histomorphological analysis was done in order to study the prostaglandins induced changes in granulosa cells of all the three categories of follicles. The acridine orange (AO) and methylene blue (MB) staining were used to study the apoptotic index in all the three categories of follicles and the collected data were analyzed by ANOVA with Duncan post Hoc test.

Prostaglandins E2 revealed positive while PGF2α showed a negative effect on the rescue of apoptosis in granulosa cells. The PGE2 treated follicles revealed a reduction in the attributes of apoptosis in granulosa cells while PGF2α showed an increased in the apoptotic characteristics.

The present study depicted that granulosa cell viability in vitro is dependent on the continuous supply of survival and growth factors and prostaglandin of E series synthesis is a crucial step in the suppression of granulosa cell apoptosis while that of F series induces apoptosis.