فهرست مطالب سیدعلی حائری روحانی

Alginate is a natural polysaccharide that can cross-link with calcium ions and form a gel. Platelet-rich plasma, as a source of growth factors, has opened new horizons for use in regenerative medicine and regeneration of damaged tissues. The aim of this study was to synthesize calcium alginate hydrogel loaded with platelet-rich plasma and examine its morphology with a field emission scanning electron microscope (FESEM).  

5 Wistar rats (200-300 g) were used in this study. The animals were anesthetized and their blood was collected via cardiac puncture and centrifuged twice to obtain platelet-rich plasma (PRP). PRP and 6% calcium chloride solution were poured into 1% alginate solution (within a mechanical stirrer) separately using an insulin syringe needle, drop by drop at the same time. After gelation and freeze-drying, its morphology was examined by field emission scanning electron microscope.

In microscopic examination, the platelets of platelet-rich plasma trapped inside the calcium alginate hydrogel were visible as platelet aggregation.

The results of the present study showed the ability of calcium alginate hydrogel loaded with platelet-rich plasma as a suitable substrate for the storage and transfer of platelets rich in growth factors for the repair and regeneration of damaged tissues.

Liver ischemia-reperfusion injury is a major complication in many clinical problems such as liver resection, liver transplantation, and trauma. p-coumaric acid from the polyphenol group obtained from cinnamic acid derivatives can reduce oxidative stress, infarction and nerve damage in the brain after ischemia-reperfusion. The present study was conducted with the aim of evaluating the protective effect of p-coumaric acid on the level of TNF-α and bilirubin due to lesions caused by liver ischemia-reperfusion injury in rats. In this study, out of 40 rats in 5 groups of 8 (sham group, control group (NC), induction of ischemia-reperfusion (IR-CO), pretreatment group with p-coumaric acid (PC) and group of p-coumaric acid with induction of ischemia-reperfusion. (PC-IR) was used. The sham and ischemia-reperfusion induction groups received 7% DMSO (2 ml/kg), the control group received 2 ml/kg of normal saline, and the pretreatment group with p-coumaric acid. and p-coumaric acid by inducing ischemia-reperfusion, they received p-coumaric acid (100 mg/kg) intraperitoneally for 7 consecutive days. Then sham, NC and PC groups underwent laparotomy. PC-IR and IR-CO groups in addition to laparotomy They were subjected to 45 minutes of ischemia and 60 minutes of reperfusion. Blood samples were taken for the biochemical analysis of liver enzymes, bilirubin and TNF-α. The results showed that ischemia-reperfusion injury causes liver function disorders by increasing the level of bilirubin and TNF-α. Also, p-coumaric acid has decreased the serum level of bilirubin and TNF-α in the liver tissue. Picoumaric acid has decreased the level of bilirubin and inflammatory factor TNF-α. p-coumaric acid with anti-inflammatory properties is a very strong agent against oxidative damage caused by ischemia-reperfusion in liver tissue.

Noise pollution is one of the hazardous factors in the environment. One of the major sources of noise pollution is urban transportation, mostly the subway system and transportation in business centers. In this study, the effects of noise pollution on blood serum proteins level of Wistar male rats were investigated. Rats were divided into 3 groups (1 control and 2 experimental, n=6). The control group was kept in standard conditions and the experimental groups were kept in Tehran Grand Bazaar and the vicinity of Sadeghiyeh metro-station (West Rose Street). After eight weeks, the blood serum proteins of each group were analyzed by electrophoresis assay. Then, the blood serum samples were investigated by Ultraviolet-Visible spectroscopy and chemometrics method (MCR-ALS). Assessment of serum proteins indicated that among 5 essential proteins in the blood, the percentage of alpha-globulins and beta-globulins displayed significant difference (p<0.05) in experimental groups. The UV-Visible spectra and chemometrics method showed that there were different species in both experimental groups as compared with the control group. Thus, the noise pollution of the areas studied led to alterations in blood proteins. Consequently, it seems crucial to take control and prevention measures for noise reduction in these areas.

Inroduction & The licorice (Glycyrrhiza glabra L.) is a perennial herb of the Mediterranean. The root of the plant is used as a food sweetener. The root extract of the plant contains glucose, sucrose, asparagine, albuminous materials, flavonoids, antioxidants and resins, which are used to treat various types of diseases including kidney damage, arthritis, inflammation of the large intestine, gastric and duodenal ulcer and cancer has been used. Glucocorticoid and non-steroidal drugs are commonly used to relieve inflammation, but these drugs have a lot of side effects, so the use of medicinal herbs to reduce inflammation is of interest to everyone. The purpose of this study was to investigate the anti-inflammatory effect of licorice ethanolic extract root on NMRI adult male mice by using xylene and carrageenan tests.
Material and Mice were divided randomly into different groups (n = 6 per group). The animal administrated with saline, licorice extract (50, 100, 150 and 200 mg/kg) intraperitoneally. Dexamethasone (10 mg/kg) was used as positive controls.
The results of this study showed that the licorice ethanolic extract significantly reduced the ear edema induced by xylene. In addition, licorice ethanolic extract significantly reduced the foot edema induced by carrageenan.
Therefore, our studies support the use of licorice extract against inflammation caused by various disorders, although further research is needed to clarify the mechanism of anti-inflammatory effect of the extract of the plant.

Nonalcoholic fatty liver disease (NAFLD) is an increasing disease in people with urban lifestyle and this disease may progress to NASH. Thymoquinone, as a powerful antioxidant and a traditional drug, was assessed in male NMRI mice with fatty liver. In this study, 40 NMRI mice were divided into four groups: healthy control, solvent, treatment 1 and 2, treating with 5 and 10 mg/ kg of thymoquinone by i.p. for 28 days, respectively. All groups were on high fat diet for four weeks except the healthy control group. At the end of the trial, the mice tissue and serum samples were collected and then biochemical and histological parameters were assessed. Our results showed that thymoquinone significantly decreased the triglyceride, VLDL, bilirubin, phospholipid, cholesterol, HOMA, and ALT levels in treatment 2 group compared to sham group (P

Adipose tissue, both white and brown, play an important role in energy homoeostasis. These tissues contain three types of adipocytes, white, brown, and beige (brown-in-white) adipocytes. The beige adipocytes of white adipose tissue, have a white fat-like phenotype and upon stimulation change to a brown fat-like phenotype, leading to increased thermogenesis, a phenomenon called browning. Obesity, a risk factor for the development of diabetes, with an increasing prevalence worldwide, depends not only on the intake-consumption ratio of calories, but also on the ratio of white-to-brown adipose tissue. Browning of white adipose tissue in humans is associated with beneficial metabolic effects. Nitric oxide deficiency contributes to the pathogenesis of obesity and diabetes. Nitric oxide is synthesized from L-arginine by nitric oxide synthase enzymes (classic pathway) and also independently from nitric oxide synthase- (nitrate-nitrite pathway). Nitric oxide production from the nitrate-nitrite pathway could potentially be used as a nutrition-based therapy in obesity and diabetes. The aims of this review is to summarize the properties of adipose tissue browning, and also, the browning effects of the nitrate-nitrite-nitric oxide. Based on literature available, administration of nitrate and nitrite can be considered to be a new treatment for obesity and diabetes. Nitrate and nitrite increase browning of white adipocytes by increasing nitric oxide and can improve metabolism.

Cardiovascular disease, which includes atherosclerosis, is one of the major diseases in the world. High sensitive C-reactive protein is the most sensitive inflammatory marker that recently its serum level predicts risk of cardiovascular disease is suggested. Both garlic (Allium sativum) and lemon (citrus limon) are used in the traditional management of CVD without a scientific evidence of the benefit of their combination. This study designed to investigate the hypolipidemic and anti-inflammatory effects of combined extracts of A. sativum and C. limon in male Wistar rats after high-fat diet for eight weeks. For this purpose, 42 adult male rats of Wistar strain, with weights between 200 and 250 gr, were divided into six groups (n=7). Group control and Exp3 was fed on normal pellet diet while group control fat, Sham, Exp1 and Exp2 fed on high-fat diet for up to 8 wk. The combined extract was administered to rats in graded doses for 4 wk and blood serum was analyzed for C-reactive protein and total cholesterol.Results are expressed as means ± SE. Significance differences of biochemical measurements were analyzed by using repeated measure and ANOVA tests to identify differences in phases and among groups. When appropriate, a Tukey posthoc was applied. Statistical significance was accepted at PA. sativum and C. limon was recognizable. However, future long-term well-designed investigations would provide valuable information to establish public health recommendations on the combined extract, taking into account both the nature of the compounds and the optimal dose, for cardiovascular health protection

The Myocardial infarction (MI) is the fail in reaching blood flow and sufficient oxygen to part of the heart. Te outbreak of MI symptoms might be various and the electrocardiography is not diagnostic in about 50% of the cases that is a potential danger for wrong diagnosis, therefore we need to test the cardiac biomarkers. In this survey the level of Troponin I serum in the diagnosis of the cardiac infarction with CK-MB serum, in 40 MI patients and 40 cardiac patients without MI of Seyyed Al Shohada Heart Hospital in Urmia has been evaluated and compared. the Troponin I through Immunoenzymometric method using AccuBind Kit and CK-MB through DGKC (German Society of Clinical Chemistry) method and IFCC (International Federation of Clinical Chemistry) using Pars Azmun Company kit through photometric method, measurement. and then Conducted the result's statistical analysis using SPSS 21 software and an independent T-test The results achieved from the comparison between the cTnI and CK-MB of MI patients and patients without MI showed that there was a significant difference between the cTnI of patients without MI and MI patients and also the CK-MB of patients without MI and MI patients at the level of (P) and the cTnI and CK-MB of MI patients is more than patients without MI. therefore their use is necessary for on time diagnosis and treatment of Myocardial infarction. In this survey the cTnI and CK-MB was compared from the viewpoint of sensitivity and specification that regarding the results the cTnI sensitivity and specification with 100% and 97.5% respectively was more than the CK-MB sensitivity and specification with 91% and 75% respectively.

Regarding to the important anti-inflammatory role of IL10 during inflammation process and hyperalgesia and edema variation during CFA-induced arthritis and also the increase of Spinal mu opioid receptor (mOR) expression, in this study researchers investigate the role of serum IL10 level on mOR expression and edema and hyperalgesia variation during different stages of Complete Freund`s Adjuvant (CFA) - induced arthritis in male Wistar rats. Mono-arthritis was induced by CFA and inflammatory symptoms (hyperalgesia and edema) were assessed on 0, 3, 7, 14th and 21st days of study. Anti-IL10 was administered during the 21 days of study in different experimental groups. mOR expression were detected by western blotting on 0, 3,7, 14th and 21st days of study. Data was analyzed by SPSS statistical software version 19 with using one way ANOVA (post hoc Tokeys). Our results showed that anti-IL10 administration in AA group (Adjuvant Arthritis) caused an increase in the paw volume and hyperalgesia until 21st of study. Our study stated that there were no significant differences in spinal mOR expression between AA and AA+anti-IL10rats. Our study confirmed that anti-IL10administration caused to hyperalgesia and edema during AA inflammation. Also these findings suggested that mOR expression increased in chronic phase of AA inflammation, however an increase in the level of spinal mu opioid receptor (mOR) expression during AA inflammation is not mediated directly via the effect of serum IL-10.

introdaction & Magnesium playes an important role in the structure and cell metabolism. In this research, anti oxidant effects of magnesium sulfate against oxidative stress effects of was investigated. In this experimewtal study 36 male adults rats were placed into4 groups, with nine rats in each group. Treatment was then carried out as follows. Group I received olive oil (intraperitoneally) and distilled water (intragastrically), and served as the untreated control animal group. Group II was the hepatotoxicity group that was given a suspension of (i.p., 0.5 mL/kg b.wt, 50% in olive oil), twice a week. Groups III received dissolved in distilled water daily via an intragastric tube(.0.15M g/kg b.wt.)Groups IV were the treatment group that received dissolved in distilled water daily, via an intragastric tube 0.15 g/kg b.wt.),with (i.p., 0.5 mL/kg b.wt., 50% in olive oil) twice a week. After a 28-day treatment period, the animals were deprived of food overnight, anesthetized by exposure to diethyl ether, and then sacrificed by decapitation. Blood ws collected from the jugular vein, and serum was separated and used for liver marker assays. Levels of ALT, AST ALP and GGT were estimated using commercial kits. The liver homogenates were used for the assay of SOD. All data were expressed as means S.E.M. Statistical analysis was carried out using one-way ANOVA followed by a Tukey post hoc test. The criterion for statistical significance was p<0.05. In the -treated control group, serum ALT, AST, ALP and GGT were significantly increased as compared with the untreated control group. In contrast, the group that also received mgso4 Showed signifi cantlyless elevated levels of ALT, AST, ALP and GGT. compared to normal levels. Liver SOD activity in -treated rats was decreased significantly when compared with the the control group. Treatment with protected this enzyme activity. The results of this study showed that magnesium sulfate has improved oxidative effect of in the liver rats because of its antioxidant properties.

Background and Morphine addiction and morphine withdrawal syndrome are of main problems in human societies. In the present study، effect of nicotine on the strength of physical and psychological dependency، produced by single and repeated doses of morphine، was investigated. Male wistar rats were dependent to morphine with single and repeated dose protocols. In the single dose protocol، rats received only one dose of morphine and 24h later were given Naloxone. In the repeated dose protocol، rats received incremental doses of morphine for 7 days and 24h after the last dose (8th day) were given Naloxone. In the single dose protocol، rats were given one dose of nicotine 30 min before Naloxone. However in the repeated doses they received nicotine 15 min before morphine for 4 days from 4th day to 7th day. 5 min after Naloxone each rat′s behavior was captured for 30 min. then physical and psychological signs of withdrawal syndrome were recorded. Results showed that injection of repeated and even single dose of morphine can produce dependency. Nicotine consumption attenuated strength of withdrawal syndrome signs، specially increasing weight excrement and total withdrawal score in single dose protocol and weight excrement increasing، weight decreasing، place aversion، and total withdrawal score in repeated dose treatment. Based on our data، even a single dose of morphine can produce dependency in rats. Conversely، Nicotine consumption attenuates strength of withdrawal syndrome signs.

Background and In this study the effects of glutathion on resumption of meiosis, in-vitro maturation and development of immature mouse oocytes was evaluated. Immature oocytes recoverd from NMRI mouse strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 immature oocytes were matured in MEM-α medium containing FCS 5%, group 1: 338 immature oocytes were cultured in MEM-α medium containing FCS 5% and 1mM glutathione, group 2: 237 immature oocytes curtured in MEM-α medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 immature oocytes were growed in MEM-α medium FCS5%, 7.5 IUhCG, 100mIUrFSH, 1mM glutathione. Fertilization and embryo development were done in T6 medium. There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumpsion of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups. The results of this study showed that glutathione (1mM) enhanced resumpsion of meiosis and in-vitro maturation and development of embryoes.

Sympathetic skin response (SSR) is a central, polysynaptic process which is attributed to sympathetic sudomotor activity. The descinding pathway presents spinal sympathetic system to sweat glands of hands and feets skin. The glands which are affected in reflex sympathetic dystrophy (RSD).

In present study, SSRs were recorded from left hands of 62 adult healthy subjects and 12 affected hands of RSD patients in relaxed, supine position at room temperature. Recording were performed by a pair of silver cup electrodes (1cm2) after stimulation of contralateral median nerve at volar aspect of wrist, all connected to a polygraph (physiograph, Narco, USA) to record at least five stimulation recording prosedure in each subject.

Comparison of mean latency, amplitude, duraion and shape of responses in RSD 'patients with normal control group showed significant differences between duration and amplitudes (P=0.0007 and P=0.0001) while the latencies were not different and the shape of responses were mostly biphasic in both RSD and normal group.

Hyperactivities of SSRs in RSD patients are due to sudomotor changes of skin resistance, the SSR may also play a role in the quantitive evaluation of patients with suspected RSD.