صادق سعادتی

Phenylhydrazine (PHZ) as a well-known hemolytic compound causes toxicity in different tissues. The present study aimed to evaluate the probable protective effects of ethyl pyruvate (EP) and vitamin E (vit E) against the damage caused by PHZ in mice kidney.
The adult male mice were divided into eight groups, with 8 mice in each group. The control group received normal saline (0.1ml/day, IP). The second group received PHZ (60mg/kg/48h, IP). The third group received PHZ along with vitamin E (100mg/kg/day, IP). The fourth group received ethyl pyruvate (40mg/kg/day, orally) along with PHZ, and the fifth group received vitamin E and ethyl pyruvate at the same dose as the previous groups, along with PHZ. The sixth group received vitamin E, the seventh group received ethyl pyruvate and the eighth group received vitamin E and ethyl pyruvate, with the same doses as previous groups, without the PHZ. After 35 days of treatment, serum and kidney tissue were sampled for serological, histological and histomorphometrical study.
PHZ treatment caused a significant elevation (P It seems that EP and vit E, as free radical inhibitors, decreased the kidney toxicity induced by PHZ in mice.

Hemolytic anemia induced by phenylhydrazine (PHZ) as a hemolytic composition can change the function and structure of liver. Therefore, the present study attempts to evaluate the protective effects of vitamin C and royal jelly co-administration against the oxidative damages and liver apoptosis induced by hemolytic anemia in adult mice.
32 adult male mice were divided equally and randomly into four groups. The first group received normal saline with a dose of 0.1 ml, IP. The second group received a dose of vitamin C (250 kg/mg, IP) along with 100 kg/mg dose of royal jelly administered orally. The third group was administered with 6 mg/100 gr, IP phenylhydrazine in 48 hour intervals. Finally, the fourth group received vitamin C and royal jelly in the doses similar to the first three groups along with phenylhydrazine with the same doses of previous groups. After 35 days, the serum and testis samples were taken and were used for serum analysis and histochemical and histomorphometric studies.
Phenylhydrazine increased the level of serum concentration of aspartate transaminase, alkaline phosphatase, alanine transaminase, malondialdehyde and lactate dehydrogenase and decreased the superoxide dismutase along with the total antioxidant capacity and serum albumin. Moreover, phenylhydrazine increased the apoptosis, the number of kupffer cells and the diameter of hepatocytes. Prescribing the royal jelly with vitamin C improved the changes of abovementioned parameters significantly.
Royal jelly with vitamin C is an antioxidant with the potential properties in preventing the oxidative damages and apoptosis induced by phenylhydrazine-induced hemolytic anemia in mouse liver.

Phenylhydrazine (PHZ) is a well-known hemolytic compound inducing intoxication in erythrocytes. Therefore, the present study aimed to evaluate the protective effects of royal jelly and vitamin C against phenylhydrazine-induced damages in mouse testicles.
In this study, 64 adult male mice were randomly and equally assigned to eight groups. The first group received normal saline (0.1ml) intraperitoneally. The second group received PHZ (6 mg/100 gr) intraperitoneally in 48-hour intervals. The third group received vitamin C (250 mg/kg/day) intraperitoneally perday a long with PHZ. The fourth group received royal jelly (100 mg/kg/day) through gavage. The fifth group received PHZ along with vitamin C and royal jelly in similar doses to the previous groups. The sixth group received only vitamin C, the seventh group recieved only royal jelly, and finally the eighth group received similar doses of vitamin C and royal jelly. After 35 days, serum and tissue samples were taken and used for histochemical (Mallory-Azan, Alkaline phosphatase, Oil red-O and PAS), and serum analyses (Testosterone, LH, FSH).
The study results revealed the histochemical changes in testicular tissue of the phenylhydrazine group, in which vitamin C and royal jelly partly improved the changes. Furthermore, serum analyses demonstrated a significant decrease in testosterone, FSH and LH levels, which this decrease was diminished by royal jelly and vitamin C.
Royal jelly and vitamin C seem to have the potential to decrease serum and tissue damages induced by phenylhydrazine via restraining free radicals.