فهرست مطالب محمد سجاد انصاری چهارسوقی

Escherichia coli is the cause of many surgical wound infections. This study was designed to investigate the antibacterial effects of hydroalcoholic extract of Capparis spinosa and pistacia atlantica on E. coli in surgical wounds using a rat model. First, Antimicrobial activity of extract of Capparis spinosa and pistacia altantica had been determined by paper disk diffusion method (to determine inhibition zone), Minimum Inhibitory Concentration (MIC) and also by Minimum Bactericidal Concentration (MBC). Then, 30 male Sprague-Dawley rats were divided randomly into three groups of treatments and control rats. A circular incision was made on the dorsal inter-scapular region of each rat. Then, rats were inoculated topically with 1.5×108 CFU of E. coli at the site of skin wounds. The extracts of Capparis spinosa and pistacia atlantica was applied to wounds twice a day during the experiment. Animals of the control group were left untreated. The results showed that the extract of Caparis spinosa and Pistacia atlantica had antimicrobial effects against Escherichia coli. The antimicrobial activity of Pistacia atlantica extract against E. coli was higher than the Capparis spinosa extract (The MIC and MBC values). Also a significant difference in the rats treated with the extracts compared to the control group. It can be concluded that the extracts of these plants have antimicrobial properties and inhibit the growth of Escherichia coli in infected wounds and thus can accelerate the wound healing process.

Achillea millefolium is one of the oldest known medicinal plants, due to the presence of certain chemical compounds are used traditional and modern medicine. One of the most important medicinal properties of Yarrow, its antimicrobial effects on a wide range of pathogens in humans and animals. The in vitro antimicrobial and antioxidant activities of the essential oil from leaves of Achillea millefolium subsp. millefolium were examined. GC-MS analysis of the essential oil resulted in the identification of fifty-eight compounds constituting 98.71% of the total essential oil of leave. The essential oil of leaves were characterized by a high number of monoterpenes borneol (12.32%), 1, 8-cineole (10.51%), β -pinene (9.33%) and α -pinene (8.82%). Regarding anti-oxidation, the investigated essential oil from leaves strongly reduced the diphenylpicrylhydrazyl radical (IC50=0.94 mg/ml). This essential oil showed antimicrobial activity as well against Staphylococcus aureus, Salmonella enteritidise, Escherichia coli, Penicillium glaucum and Saccharomyces cervesiae (MIC values 0.45-7.20 mg/ml). This study confirms that the essential oil from leaves of Achillea millefolium possessed antioxidant and antimicrobial properties in vitro.