پوران اردلان

Angiogenesis plays an important role in physiological (fetal) and pathological conditions (some diseases such as cancer). Many researchers are interested in encapsulating anti-angiogenic compounds and using them to inhibit the process of angiogenesis in the fight against various diseases. This study evaluated the antiangiogenic properties of Carum Carvi essential oil  nanoemulsion using the CAM method.

In order to investigate the angiogenesis effects of Carum Carvi  nanoemulsion, on the 2nd day, a window was created on the eggs and on the 8th day, Carum Carvi  nanoemulsion with different doses (12.5, 25 and 50 µg/ml) were used and on the 12th day, the rate of angiogenesis was investigated. The stereomicroscope was used to take image of the treatment area, and vascular factors were examined with Image J software.

The angiogenic results in the CAM assay showed a decrease in the length and number of vessels in chorioallantoiec membranes in the treated samples compared to the untreated. The reduction in height and weight of the treated embryos compared to the untreated was also shown in this study (p <0.05).

The anti-angiogenic activity of Carum Carvi nanoemulsion implicates its possible application for diseases where inhibition of blood vessel formation is desired, so it can be recommended as a substance for further studies about cancer.

Cancer is a kind of genetic disease caused by DNA mutation which makes disorder in normal pattern of division and differentiation of cells and eventually formation of a neoplasm. Breast cancer is the most common malignancy and the second leading cause of death in women aged 35-55 years. Surgery, radiotherapy and chemotherapy are the common methods of controlling cancer, but the side effects and lack of positive results especially in metastatic tumors has led us discover new treatments. Nowadays, nanotechnology has helped us to find and develop new therapies. The purpose of this study was to characterize the antioxidant and apoptotic properties of zinc oxide nanoparticles biosynthesized by Amaranthus cruentus plant on breast cancer cells (MDA-MB-231).

The Zinc oxide nanoparticles were green-synthesized by the extract of Amaranthus cruentus leaves. Size and morphological characteristics of ZnO NPs determined by DLS tests, FESEM and TEM. To investigate the effect of ZnO nanoparticles on induction apoptosis, cancer cells were seeded in T25 flasks and treated with different concentrations of nanoparticles (15, 30 and 60 μg/ml). After 48 hours, gene expression changes of Bax and Bcl-2 was investigated by Real time PCR technique. SPSS software and one-way ANOVA test were used to analyze the data. At the end, comparison of means did by least significant differences (LSD) method.

The DLS test showed the average size of the synthesized ZnO NPs is about 30 to 38 nm. The results of gene expression by Real time PCR technique showed that ZnO nanoparticles reduced anti-apoptosis gene expression Bcl-2 and increases pro-apoptotic gene expression in MDA cell line.

In general, the results obtained from this study can claim that ZnO nanoparticles have anti-cancer properties and can be introduced after further studies as candidates for cancer treatment in the field of medicine and pharmacy.

One of the important aspects of nanotechnology for use in biology is the production of nano materials with controllable size, chemical characteristics and dimensions. It can be said that the purpose of nanotechnology is to control each atom and molecule. It is now known that nanoparticles are capable of destroying cancerous cells without side effects on normal cells. Liver cancer is the fifth most common cancer in the world and the third most common cause of cancer deaths in the world. The purpose of this study was to investigate the anti-inflammatory properties of silver nanoparticles synthesized by the Amaranthus Cruentus plant on Hep G2 liver cancer cells.

The studies in this research included in vitro experiments. The anti-inflammatory effect of silver nanoparticles produced by the Amaranthus Cruentus plant was evaluated using the Real-Time PCR technique and assaying the expression of IL-1b and IL-10 genes at concentrations of 50, 100 and 200 μg/ml.

The findings from the gene expression showed that the silver nanoparticles produced by the Amaranthus Cruentus plant significantly reduced anti-inflammatory activity by decreasing the expression of the IL-1b pre-inflammatory gene expression at the level of P <0.001 and increasing the expression of the anti-inflammatory IL -10 meaningful at the level of P <0.001.

Based on the results of this study, silver nanoparticles of the Amaranthus Cruentus plant have anti-cancer properties, and the expression of the gene leading to inflammation, IL-1b, decreased meaningfully. In addition, there was a significant increase in the expression of the IL-10 gene. Given these features, it is likely that the use of these nanoparticles, after supplementary studies, can be suggested as complementary data in the treatment of cancer and could have other biomedical applications.

Free radicals are one of the causes of cancer. In case of the disease, some cells of the body progress to the surrounding tissues and divide without stopping. The use of natural antioxidants plays a positive role in maintaining the health. Cancer and the presence of free radicals in the body motivated researchers to study substances that may eliminate them. The purpose of this study was to investigate the cytotoxicity and antioxidant effect of Carum Carvi essential oil nanomulsion. In this study, antioxidant activity was measured by DPPH and ABTS in different concentrations of nanoemulsion (200, 400, 600, 800 and 1000 μg/ml). Also, cytotoxicity was investigated by MTT method on breast cancer cells (TUBO) and normal cells (L929) at different concentrations of nanoemulsion. The results of the antioxidant, DPPH and ABTS tests with IC50 indicated the antioxidant properties of Carum Carvi essential oil nanoemulsion. The IC50 showed anticancer activity of the nanoemulsion, however low concentration of nanoemulsion has no significant effect on normal cells. The results suggested that Carum Carvi essential oil nanoemulsion has the ability to inhibit the proliferation of cancer cells and also inhibit free radicals.

The use of nanoparticles to inhibit the growth and proliferation of cancer cells is one of the achievements of the nanotechnology science. Studies have shown that silver nanoparticles have cytotoxic effects on the growth of cancerous cells. On the other hand, the antioxidant properties of this nanoparticle have been proven in many studies. Therefore, in this investigation, antioxidant capacity and cytotoxicity effects of silver nanoparticles synthesized from Root of Persicaria bistorta L (Bi/Ag-NPs) on liver cancer cells (HepG2) compared with normal skin cells (HDF) were studied. In addition, ABTS and DPPH free radical scavenging capacity of Bi/Ag-NPs was evaluated using colorimetric tests.   In order to evaluate the cytotoxicity effects, first the cancerous and normal cells were cultured and treated with various concentrations of Bi/Ag-NPs and finally, the cell survival rate was estimated using the MTT assay. The antioxidant activity of Bi/Ag-NPs was evaluated according to the amount of ABTS and DPPH free radicals inhibition.     The results showed that Bi/Ag-NPs inhibited cancer cells with an IC50 value of about 3 μg/ml, while at this concentration they were ineffective on normal cells (IC50: 50 μg/ml). The scavenging of ABTS (IC50: 15 μg / ml) and DPPH (IC50: 20 μg/ml) free radicals confirmed the antioxidant properties of Bi/Ag-NPs.   The cytotoxic and antioxidant results show that Bi/Ag-NPs can be used as auxiliary agents for treating many diseases that are caused by oxidative stress and other biomedical applications.

Angiogenesis is a key event during tumor growth. Since tumor cells require more oxygen and glucose than normal cells and due to tumors quick growth, new blood vessels rapidly increase to meet the nutritional requirements. Hence, targeting and inhibiting the formation of new blood vessels can reduce the nutrition and access of tumor cells to oxygen, which also inhibits tumor growth.. In the present study, the effects of coated silver nanoparticles with Persicaria bistorta on chick embryo chorioallantoic membrane and its antioxidant properties have been investigated.
The fertilized Ross eggs were distributed randomly and equally into 6 groups. Two groups were considered as control and laboratory control (treated with solvent). In the second day of incubation, a window was created on the eggs, and then in the 8th day after incubation, samples were exposed with various doses (60, 120, 240, 480 µg/ml) of the Ag-NPs. On the 12th day, photographs were taken and the length and number of vessels were measured using Image J software. The length and weight of embryos were measured and compared. The data were analyzed by SPSS software and one way ANOVA at the significance level of p Based on the results, the silver nanoparticles at a concentration of 60 μg / ml reduced the length and number of blood vessels significantly (P Accordingly, the use of silver nanoparticles can be considered as a potential treatment against pathological angiogenesis and solid tumors targeting blood vessels.

Nanotechnology and nanoparticles have significantly been considered for their potential in various fields including medicine and treatment. Silver nanoparticles are the most important nanoparticles that play an important role in treatment of cancer. Previous studies showed that silver nanoparticles have several properties such as antioxidants, anti-cancer and antibacterial. Therefore, in this study, the antioxidant properties of silver nanoparticles synthesized by Rubina tinctorum L (Ru-AgNPs) were investigated, using standard protocols. Subsequently, the cytotoxicity effects of the synthesized nanoparticles were evaluated on cancer cells (HepG2) compared to normal ones (HDF).
In this study, the antioxidant effect of Ru-AgNPs was evaluated using ABTS and DPPH free radicals scavenging assay. The MTT procedure was used to evaluate the cytotoxic properties of the nanoparticle against two cell lines examined 24 hours after exposure.
Findings: The results showed that Ru-AgNPs was able to inhibit ABTS and DPPH free radicals depending on the concentration. The MTT results demonstrated that this nanoparticle can inhibit liver cancer cells in very low concentrations (IC50: 6µg/ml), but does not have an inhibitory effect in similar concentration on normal cells (IC50: 100µg/ml).
Conclusion & Our results show that Ru-AgNPs has an antioxidant effect and is able to inhibit the HepG2 cells in a low concentration too, but it does not have any toxic effects in a similar concentration on HDF cells, which makes this nanoparticle a suitable candidate for use in inhibiting cancerous cells

Angiogenesis occurs in physiologic (wound healing) and pathological conditions and plays an important role in tumor growth, progression and metastasis. Therefore, the inhibition of angiogenesis can be an important approach to treat cancer diseases. In recent years, the use of nanoparticles has been developed to control cancer cells and prevent tumor growth. Various studies have shown the role of nanoparticles in reducing angiogenesis and inhibition of cancer cells. So, in this study, the anti-angiogenic effect of silver nanoparticle synthesis by Rubina tinctorum L (Ru-AgNPs) on Chicken Chorioallantoic Membrane (CAM) was investigated.
In an experimental study, 60 eggs were randomly divided into 6 groups including control, laboratory control and 4 treatment groups (10 eggs/group). On the second day of incubation, a window was created on the eggs. On the 8th day, the chorioallantoic membrane of samples was treated with different concentrations of nanoparticles and on the 12th day, the image was taken from all samples. Finally, the number and length of vessels on the chorioallantoic membrane and following that weight and length of the embryo from crown to squattiest were measured. Quantitative data were analyzed using LSD-test.
The results showed that the Ru-AgNPs significantly reduced the length and number of blood vessels in the treated groups compared to the control group. Also, the comparison of length and weight of embryos in treated samples with control group showed that the Ru-AgNPs had an inhibitory effect on the length of the embryos, but the weight did not differ significantly in the treatment with the control group.
Considering the inhibitory effect of the Ru-AgNPs on the angiogenesis of the chorioallantoic membrane, with further studies, this compound can be used to control and inhibit pathological angiogenesis in many diseases such as cancers.

The biosorption of reactive azo dyes (Direct red 81) found in textile wastewaters by activated carbon prepared from sonchus fruit plant has been investigated. Batch experiments were carried out for the biosorption of dyes molecules onto biosorbent at different temperature. The biosorption kinetics was found to follow pseudo- second order kinetic model with high degree of correlation coefficient (R2>0. 99). The equilibrium isotherms of biosorption and kinetics were studied. The biosorption equilibrium data were analyzed by using Langmuir and freundlich isotherm models and the results have shown that biosorption behavior of this dye could be follow to Langmuir isotherm model. Various parameters such as contact time، initial dyes concentrations، temperature، stirring speed and does of biosorbent were taken into account، and promising results were obtained. Furthermore، it was also found that the sonchus fruit plant can using as a low cost material for azo dye biosorbtion.

Density functional theory (DFT) at B3LYP/6-311G* level was employed to calculate antioxidant activity for Trolox[(+) -6-Hydroxy-2،5،7،8-tetramethylchromane-2-carboxylic acid] and Substituted Trolox. Bond dissociation energy (BDE)، HOMO-LUMO Gap energy، O-H charge differences and O-H bond lengths were calculated. The results are shown that the BDE values of substituated Trolox range from about 74 to 79 kcal/mol، demonstrating that Trolox is an effective chain-breaking antioxidant that prevents lipid peroxidation. Also The small value of gap and the distribution of π-like frontier orbital (HOMO and LUMO) delocalized through the whole molecule (Table 3) corroborate that Trolox and substituted Trolox could be a reactive systems.