مقالات رزومه دکتر عبدالرضا ستوده جهرمی

Mismatch blood group antigens between blood donor and recipient increases the risk of alloimmunization. The aim of this study was to detect the frequency of unwanted alloantibodies against blood group antigens in beta-thalassemia patients receiving continuous blood transfusions in Jahrom City.

In this descriptive study, a total of 79 patients with beta-thalassemia were enrolled. Alloantibodies were evaluated using antibody screening and identification (panel test) methods. Statistical analysis was performed with SPSS version 23. All data were statistically analyzed using descriptive statistics methods and Fisher's exact test.

In this study, 10 patients (12.65%) had alloantibodies against red blood cells (RBCs). The most commonly detected alloantibodies in patients were anti-K (50%), anti-E (20%), and anti-c (20%). The rate of alloimmunization was significantly lower in transfused patients with leukoreduced RBCs compared to those transfused with non-leukoreduced RBCs (P<0.001). Patients with O+ blood group produced the most alloantibody, which was not statistically significant (p=0.61). There was no statistically significant correlation between the prevalence of alloantibody with the sex and age variables (p>0.05).

Based on the results, the most commonly detected alloantibodies were against Kell and Rh (E, c) blood group systems, which can be useful in deciding to store blood components with rare blood groups.

Immune system disorders as one of the major problems in dialysis patients increase their susceptibility to pneumococcal pneumonia. The present study was conducted for the first time in Iran with the aim of determining the prevalence of natural acquired immunity to Streptococcus pneumoniae in hemodialysis patients in Jahrom, Iran.

This cross-sectional descriptive study was performed on 89 hemodialysis patients in Jahrom-Iran, 2022. The presence of anti-pneumococcal antibodies in the serum of patients was assessed by ELISA method. Demographic information, mean history of hemodialysis treatment and number of times per week were extracted from patients' records. Data were analyzed using SPSS Statistics-23 statistical software (IBM, USA) using descriptive statistics, chi-square and Fisher's exact test.

The prevalence of immunity to Streptococcus pneumoniae in dialysis patients was 20.22%. There was a significant difference in the prevalence of pneumococcal immunity between participants in terms of age groups, sex and number of dialysis sessions per week (p<0.05) but there was no statistically significant relationship with the duration of dialysis treatment (p=0.09).

Prevalence of this type of immunity, which indicates a history of pneumococcal infection in dialysis patients in Jahrom. Due to the ability to prevent this infection by pneumococcal vaccination, the need for Iranian dialysis patients for pneumococcal vaccination is recommended.

Hemodialysis patients are at risk for viral hepatitis due to invasive procedures and frequent contact with blood and body fluids, this increases the importance of viral hepatitis in patients undergoing hemodialysis. The aim of this study was to determine the serological prevalence of hepatitis G virus (HGV) and its associated factors in hemodialysis patients in Jahrom-Iran.

This cross-sectional descriptive study was performed on 91 hemodialysis patients in Jahrom-Iran, 1400. The presence of anti-HGV antibodies in the serum of patients was evaluated by ELISA method. Demographic information, mean history of hemodialysis treatment and serum levels of liver enzymes were extracted from patients' records. The obtained data were statistically analyzed by SPSS-16 statistical software using descriptive statistics, T test and chi-square.

The prevalence of anti-HGV IgG and IgM were 5.46% and 0%, respectively. The prevalence of anti-HGV IgG was statistically significantly related to the history of blood transfusion and mean serum levels of ALT, AST, ALP (P<0.05), but there was no statistically significant relationship with the mean age and sex of patients (P>0.05).

The prevalence of hepatitis G virus in hemodialysis patients in Jahrom is equal to the average prevalence obtained in hemodialysis patients in other parts of the country. Further research is recommended to determine the role of this virus in causing hepatitis in hemodialysis patients.

Despite advances in diagnosis and therapy, cancer is still the main cause of death in world. Vincristine is one the major drug in tumor treatment. However, the anti-lymphoma effects of Vincristine on micro-RNA expression are not clear, especially in B cell lymphomas. The aim of the present study was to evaluate the effects of Vincristine on miRNA-15a3p and miRNA-15a5p genes expression Epstein‐Barr‐Virus (EBV)-infected transformed B cell lymphoma. In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with Vincristine (0.05-50 µM), for 24-72 hours. MTT, flow cytometry and real-time PCR techniques were used for cell viability, and cytotoxicity evaluations and miRNA-15a genes expression, respectively. Vincristine significantly inhibited proliferation and induced cell death in EBV infected cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05). The results showed that cytotoxic effects Vincristine on CO 88BV59-1 LCL is independent on miRNA-15a3p and miRNA-15a5p expressions.

People who have multiple transfusions, such as Beta-thalassemia patients, are at risk for the hepatitis G virus (HGV) infection. The aim of the study was the determination of serological prevalence of HGV and its associated factors in beta-thalassemia major patients in Jahrom-Iran.

This cross-sectional study, was done on 91 beta-thalassemia patients referring to the thalassemia center, Jahrom-Iran, 2021. Blood samples were collected from the patients and anti-HGV antibodies were evaluated by ELISA methods. Serum levels of ALT, AST, ALP and demographic data were extracted from patients' medical records. Descriptive analysis, X2, and T-test were used for statistical analyses by SPSS-16.

The prevalence anti-HGV IgG and IgM were 17% and 5%, respectively. There were significant relationships between the frequencies of anti-HGV antibodies and serum level of ALT, ASL, ALP and also with the history time of transfusion (P<0.05). But, there were no significant relationships between the frequencies of anti-HGV antibodies with patients' sex and the mean of age (P>0.05).

HGV is one of the etiologic factors of hepatitis in thalassemia patients in Jahrom. Further comprehensive and molecular-based studies are recommended to explore the prevalence of HGV and its role in hepatitis thalassemia patients.

Despite advances in diagnosis and therapy of cancer, it is still the main cause of death worldwide. Cyclophosphamide is one of the major drugs for lymphoma treatment. However, the anti-lymphoma effects of cyclophosphamide on micro-RNA expression are still unclear, especially in B cell lymphomas. The aim of the present study was to evaluate the effect of cyclophosphamide on miRNA-15a genes expression in Epstein‐Barr‐Virus (EBV)-infected transformed B cell.

In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with cyclophosphamide (0.05-50 µM), for 3 days. The cell viability and cytotoxicity were evaluated using MTT, and flow cytometry techniques, respectively. Also, miRNA-15a genes expression were determined by real-time PCR.

Cyclophosphamide significantly inhibited proliferation and induced cell death in the cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05).

The results showed that cytotoxic effects of cyclophosphamide on CO 88BV59-1 LCL is not related to miRNA-15a3p and miRNA-15a5p expressions.

Despite advances in diagnosis and therapy, cancer is still the main cause of death worldwide. Crocin, the major carotenoid in saffron, exhibits potent anti-tumor effects in breast, pancreas, and gastric tumors. However, the anti-lymphoma effects of crocin are still unclear, especially in B cell lymphomas. The present study aimed to evaluate the cytotoxic effects of crocin on EBV-infected transformed B cell.

In this study, the effects of crocin on CO 88BV59-1 LCL – an Epstein‐Barr‐Virus (EBV) Infected Transformed B cell was examined. The cells were treated with crocin (0.2-200 µM), for 3 days. Cell viability and apoptosis were evaluated using MTT, and flow cytometry techniques respectively. Also, miRNA-15a genes expression were determined by real-time PCR.

Crocin significantly inhibited proliferation and induced apoptosis in EBV infected cell-line in a dose and time-dependent manner (P<0.05). There was no significant change in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05).

The results suggested that crocin can be considered for preclinical and also clinical testing in EBV-associated B cell lymphoma treatment. The results showed that due to the effect of crocin in reducing the survival and increasing apoptosis of lymphoma cells and also due to the lack of change in the expression of miRNA-15a, it can be considered as a therapeutic candidate in the treatment of EBV-associated B cell lymphoma.

lectures of the professors are important، because، a great volume of Outlook and experiences of professor in these classes will be transferred to students. The Realization of above mentioned is active and continued participation of students in theoretical Classroom. The objective of this study is evaluation of effective factors on the presence and absence in theoretical classrooms، from the viewpoints of medical students of Jahrom medical university. This is analytic descriptive study. The statistical population is 254 of medical students from basic، physiopathology and clinical sections، 181 questionnaires were completed and returned. Data was analyzed by SPSS15 and Central index، dispersion، Independent t-Test and One way ANOVA. Significant level was considered less than 0. 05. The mean age (standard deviation ±) of 181 students who responded to the questionnaire، was 21. 48 (± 2. 2) that 66. 3 percent of them were female. Among the 12-fold factors that affect on presence in theoretical classrooms، the maximum mean score were about application of the theory class presentation in hospital and among the 12-fold factors that affect on absence of theoretical classroom، the highest score related to inappropriate teaching method of professors. Application of Contents presentations at bedside patient، mastery of professor on the subject، important and the necessity of subject can be effective on the continuity of their presence in classrooms. Inappropriate teaching method by professors، Low efficiency of classes and fatigue from the previous classroom are presented by medical students as important factors that encourage the students to absent of classrooms.

Hepatitis C virus (HCV) is a major cause of chronic liver disease among chronic renal failure (CRF) patients undergoing maintenance hemodialysis.The Management of HCV-related liver disease is a major health concern in patients with end-stage renal disease (ESRD) undergoing haemodialysis. To investigate the prevalence of HCV infection in patients on haemodialysis in Jahrom This cross-sectional study was conducted on all haemodialysis patients in Jahrom in April- May 2006. All the patients were screened for anti-HCV antibodies, using ELISA and confirmed by RIBA methods. The findings of this study revealed that 3 patients(8.8%) were anti-HCV positive as confirmed by Elisa method and z (5.9%)as confirmed by Riba method.  The seroprevalence of HCV among  the haemodialysis patients of Jahrom was 5.9% ,being is lower than that of  haemodialysis patients in other countries.