ali jebali

Our aim was to investigate the expression of miRNAs, C-reactive protein as a blood inflammation marker, and alanine aminotransferase as a tissue inflammation marker, in recovered and not-recovered COVID-19 patients.

This cross-sectional project was conducted at three medical centers in Iran from December to March 2021. In total, 20 confirmed cases of COVID-19 with grade III severity and 20 healthy subjects were enrolled in the study. Subsequently, the neuroinflammatory expression of miRNAs (miR-199, miR-203, and miR-181), C-reactive protein, and alanine aminotransferase was investigated during hospitalization from week 0 to week 2.

Among COVID-19 subjects who did not recover, the expression levels of miR-199, miR-203, and miR-181 were decreased, while the levels of C-reactive protein and alanine aminotransferase increased during hospitalization. Conversely, in recovered COVID-19 subjects, the relative expression of miR-199, miR-203, and miR-181 increased and the levels of C-reactive protein and alanine aminotransferase decreased during hospitalization.

The expression pattern of neuroinflammatory miRNAs depends on whether the COVID-19 patient is recovering or deteriorating. Their expression is downregulated in COVID-19 patients who do not recover and upregulated in those who do recover.

The post-harvest damage to fruits is estimated to be about 10-30% of the total products, which reaches up to 30-50% in some perishable fruits. About 25 species of fungi and bacteria including Botrytis spp. and in particular Botrytis cinerea are known to contaminate fruits, vegetables and ornamental greenhouse plants. The aim of this study was to investigate the antifungal activity of different concentrations of Zataria multiflora essential oil (ZEO) against B. cinerea.

The ZEO was extracted through steam distillation and analyzed by gas chromatography-mass spectrometry. The strawberries packages were exposed to ZEO with different concentrations (0, 200, 400, 600, and 800 ppm) and satarch nanoparticles. The exposed fruits were kept for 24 days at two temperatures of 20°C and 4°C.

The ZEO decreased mycelium growth even when only 200 ppm of it was added to each container. The response was dose-dependent, so that the 800 ppm dose of ZEO showed complete inhibitory effect. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values for ZEO against B. cinerea were 200 µg/mL and 500 µg/mL, respectively. Additionally, ZEO preserved the sensory characteristics.

The ZEO may be effectively used in packaging of strawberry to increase its shelf life by inhibition of B. cinerea.

Niosome is a lipid carrier that can resolves some of the challenges facing the delivery of herbal medicines to tissues. The aim of the present study is to fabricate and evaluate the physiochemical properties of niosomal carriers containing Hedera helix extracts and evaluation of its toxicity to the HT29 cell line of colon cancer.

Two niosomal formulations F0 (30% cholesterol and 70% tween60) and F1 (30% cholesterol and 70% spin60) containing extract of Hedera hilix were made using the thin film method. Then, according to the amount of drug loading, one of the formulations was selected. The pattern of drug release under normal and cancerous cell conditions, size and surface charge of the nanoparticles (using DLS) and the appearance of the nanoparticles (using SEM) of the selected formulation were investigated. Finally, the toxicity of the niosomal system containing extracts and free extracts on HT29 cell line was evaluated by MTT assay and niosomal system entry into HT29 cells was evaluated by system and cell staining.

Extract loading percentage, size and zeta potential for the selected formulation (F1) containing the extract were 95/43±2/43%, 132/5nm and -41/47±2/69mV, respectively. Release of the extract from the niosomal system is slow within 48 hours in normal and cancerous cell conditions. The appearance of the nanoparticles was smooth and spherical and extract niosomal had more toxicity to HT29 cell line colon cancer than free extract.

The niosomal formulation of this study can be recommended for further research in colon cancer with respect to its physicochemical properties.

Applying of a new indicator in food packaging can be effective to inform consumers about the freshness and quality of the products.

In the current study, a new milk freshness label was investigated containing beetroot color and multi layers of polystyrene. The label characteristics were investigated by estimating color number, release test, and scanning electron microscopy (SEM). The total bacterial count, pH, lactic acid concentration in milk, and label color number were measured using standard plate count, pH meter, titration of acidity, color analysis software, and UV spectrophotometry on days 0, 3, 4, 5, 6, and 7 at refrigerator temperature (4 ± 0.2°C).

The label reacted to total bacterial count and pH changes with a visible color during milk spoilage. A positive correlation was found between the label color changes, total bacterial count, and pH. The color of label turned from dark red to light brown, which was related to the chemical changes and bacterial count of milk.

According to this simple, visible, and affordable label, the shelf life of pasteurized milk was estimated as five days.

The rainbow trout fish is susceptible to spoilage due to its high content of unsaturated fatty acids. It should be kept at low temperature to reduce microbial, enzymatic, and oxidation reactions. The purpose of this study was to design a packaging that contains a pH indicator for monitoring freshness of the rainbow trout fish during storage at refrigerator.

The indicator contained agarose as the carrier, bromocresol green as pH indicator, and silica as surface provider. It was covered by polypropylene film and attached inside the package. Freshness of the trout stored in the refrigerator was assessed by chemical (total volatile basic nitrogen and pH) and microbiological (total viable count) methods.

The pH of fish gradually decreased after the third day since color of the indicator changed from yellow to green on day 3 and then to blue on day 6. The indicator's response was correlated with changes in the microbial population and also with levels of total volatile basic nitrogen and pH. The results showed that the designed indicator was sensitive to different pH levels and could be applied as part of the intelligent packaging system.

The freshness indicator worked well before the expiry date of fish, which makes it suitable for food quality assessment. So, this indicator can be used for real-time monitoring of packaged fish freshness

Intelligent packaging is a type of packaging which could control the environment around the food and inform consumers about food condition. The objective of present research was to design a simple pH-sensitive label in chicken package which show the spoilage by changing its color. The Bromocresol green, a pH-sensitive color indicator, has been used to detect chicken spoilage and attached to the inner side of the package. The analysis including chemical (pH and TVBN), microbial and sensory analysis were done at the intervals of 0, 3, 5, 7, 8 and 10 days storage at 4 °C. The TVB-N was 19 mg/100g in the first day and measured 29 mg/100 g on the 8th day and 46 mg/100g of on the last day, respectively. According to achieved results, the changes of BCG color due to chicken spoilage were detectable by naked eye. The bacterial count was about 6.62 Log10CFU/g. On the last day, the indicator’s color became blue and the total count reached about 7.20 Log10CFU/g. There was a significant relationship between Total Volatile Nitrogen Base (TVBN), pH, microbial and sensory analysis in all studied samples with the changes in indicator’s color during storage. Due to increase in TVBN during storage, the Bromocresol Green (BCG) indicator’s color changed from yellow to blue. Thus, the BCG indicator can be employed as an inexpensive and simple label to show the freshness of chicken meat.

Staphylococcus aureus, Salmonella enterica, Escherichia Coli (E. Coli) and Listeria monocytogenes are considered as important foodborne pathogens. Pistachia atlantica sub sp. Kurdica, called wild pistachio, has been known as an antimicrobial compound. The aim of this study was to determine the antimicrobial activity and chemical composition of this essential oil (EO) on some of foodborne pathogens. The EO of Pistachia atlantica was obtained by hydro-distillation and analyzed by GC-MASS. The antibacterial effects of Pistachia atlantica were evaluated at two concentrations of 10 and 15 µL against Staphylococcus aureus, E. Coli, Salmonella enterica, and Listeria monocytogenes using disk diffusion method. The analysis was done by SPSS. In the current study, α-pinene (92.5%) and ß-pinene (1.62%) were the main components of Pistachia atlantica EO. The EO was most effective on Salmonella enterica, whereas, its effect on Listeria monocytogenes was the weakest. The results showed a significant difference in reducing Salmonella enterica in comparison to others (P < 0.05). The EO has inhibitory effects on the studied bacteria. Therefore, this EO can be used as a natural preservative to extend the shelf life of foods.

Recent achievements in stem cell biotechnology, nanotechnology and tissue engineering have led to development of novel approaches in regenerative medicine. Azoospermia is one of the challenging disorders of the reproductive system. Several efforts were made for isolation and culture of testis-derived stem cells to treat male infertility. However, tissue engineering is the best approach to mimic the three dimensional microenvironment of the testis in vitro. We investigated whether human testis-derived cells (hTCs) obtained by testicular sperm extraction (TESE) can be cultured on a homemade scaffold composed of electrospun nanofibers of homogeneous poly (vinyl alcohol)/human serum albumin/gelatin (PVA/HSA/gelatin). In this experimental lab study, human TCs underwent two steps of enzymatic cell isolation and five culture passages. Nanofibrous scaffolds were characterized by scanning electron microscopy (SEM) and Fourier- transform infrared spectroscopy (FTIR). Attachment of cells onto the scaffold was shown by hematoxylin and eosin (H&E) staining and SEM. Cell viability study using MTT [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2H- tetrazolium bromide] assay was performed on days 7 and 14. Visualization by H&E staining and SEM indicated that hTCs were seeded on the scaffold. MTT test showed that the PVA/HSA/gelatin scaffold is not toxic for hTCs. It seems that this PVA/HSA/gelatin scaffold is supportive for growth of hTCs.

Aspergillus flavus is a toxic contaminant in foods, which can induce mutagenic, teratogenic, and carcinogenic effects. In last decades, synthetic fungicides have been used for inhibition of fungal growth in plants. The public attention was also attracted to contamination of food chain by these chemicals. Therefore, in the current study, we decided to use Zataria multiflora (ZM) essential oil to inhibit the Aspergillus flavus growth. The essential oil from ZM was obtained by hydro-distillation and analyzed by GC/MS. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of ZM essential oil were determined at different concentrations (0-1000 ppm). In this study, Carvacrol (33.45%) and Thymol (34.44%) were the most frequent compounds of the ZM essential oil. The minimum inhibitory and fungicidal concentrations were 100 and 400 ppm, respectively. The presence of phenolic compounds such as Thymol and Carvacrol, as the major components of ZM essential oil inhibits the Aspergillus flavus growth. Furthermore, this essential oil has a very strong anti-bacterial effect. Considering these results, it seems that ZM essential oil is potentially an appropriate natural alternative for controlling Aspergillus flavus fungus.

Exposure to noise pollution in the workplace is one of the most common occupational hazards and causes hearing loss. One of the ways to control voice in the control industry is by audio absorbers. Nanoparticles can be used to composite materials. The purpose of this study was to design and construct acoustic panels based on gypsum, silicon oxide nanoparticles, hydrolyzed cotton and nanodye, as well as to compare the absorbance of sound by them at various frequencies.
In this study, the nanocomposite consisting of gypsum, hydrolyzed cotton, silica nanoparticles and nanodye was prepared by simple mixing and heat treatment, and then its sound absorption coefficient was investigated. The absorption coefficient was evaluated at frequencies of 250 to 5000 using static waves and in accordance with ISO 9803-1.
The results showed that the maximum difference of the absorption coefficient was 10% at 2500 Hz, and it was related to a sample consisting of gypsum, nano silica, hydolized cotton, and nanodye, compared to a pure plaster sample. The highest absorption coefficient (0.46) of the sample was from gypsum, nano silica and hydolized cotton at a frequency of 5000 Hz.
The results of this study showed that with increasing frequency, the absorption coefficient increased, but the absorption coefficient was decreased by adding nanoparticles and nanodye.

Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cells. The aim of this study was to evaluate the use of magnetic nanoparticles containing iron oxide and silver (Fe3O4@Ag core-shell nanoprobe) as a contrast agent for the detection of ovarian cancer cell line ovcar-3.
Co-precipitation method was used for synthesis of Fe3O4Ag nanoprobe which was then characterized by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and scanning electron microscope (SEM). To evaluate the ability of this nanoprobe in detection of the ovcar-3 cell line by MRI, the cells were exposed to different (5 to 50 µg/mL) concentrations of Fe3O4@Ag nanoprobe before contrast intensity calculation by MRI.
SEM images revealed that Fe3O4@Ag nanoparticles are spherical, about 100 nm. FTIR showed strong absorbance picks belonging to the stretching vibration of Ag and Fe-O. It was found that contrast intensity of Fe3O4@Ag nanoprobe decreases as concentration decreases. Statistical analysis revealed significant difference in concentrations of 30, 40 and 50 μg/mL, compared to control (p This novel magnetic nanoparticle can be used as an effective contrast agent for improving MRI in detection of ovarian cancer cells. The sensitivity of this contrast agent may be improved by binding to targeting molecules such as antibody and aptamer.

Alzheimer's disease is a progressive cerebrovascular disease with irreversible damage, which gradually degrades the mental abilities of the patient. In this study, amyloid peptide was exposed to seven different compounds in the canaboid extract and then, the structure and thermodynamic parameters were evaluated.
In this study, the three-dimensional structure of beta-amyloid peptide in patients with Alzheimer's disease was prepared from the rcsb database. The main components of canaboid extract were obtained from CHEBI database too. Then, amyloid peptide was subjected to each of the canaboid extract molecules in the molecular dynamics simulation software, and its structural and thermodynamic characteristics were evaluated over 3000 picoseconds.
Comparison of seven different compounds of cannabis extract showed that all cannabis extract compounds were able to change the structure of amyloid peptide. Each compound was able to change the effective surface and volume, and the level of lipophilicty of amyloid peptides.
The ability of these compounds to change the volume and surface is effective in preventing amyloid peptide accumulation. The findings of this study should be investigated in a laboratory scale and then on the human body to detect the efficacy of canaboid extract components exactly.

Sound is one of the most important work-related risk factors and most widespread physical factors threatening the health of workers. Almost one of the best ways to control the sound, is sound absorber. Due to the extensive entrance nano in various fields of science one their applications design and use sound-absorbing panels containing nanoparticles.The aim of this study to determine the sound absorption coefficient of nanocomposites, containing aluminum nanoparticles at different percentages.
This study had an experimental – Laboratory procedure. First of aluminum particles (100 nm), wood chips and glue polyvinyl acetate weight was measured the ratio of identified and wellmixed. In the next step, the obtained mixture is poured into the mold Heat resistant and transferred to 37 ° C. After the preparation ofmentionednanocomposites then sound absorption coefficient values were determined by acoustic impedance tube at four frequencies, 250, 500, 1000 and 2000 Hz.
The Comparison between the sound absorption coefficient of four weight percentage (1, 2, 3 and 4% of 100nm-aluminum nanoparticles) of aluminum indicated thatthealuminum nanocomposite had the highest sound absorption coefficient of weight percentage 4% at frequencyof 2000 HZ. While theallweight percentagesaluminumnanocomposites was seen the lowest sound absorptioncoefficient at frequency of 500 Hz.
The results showed that the increasing of the percentage of aluminum nanoparticles led to increase of sound absorption coefficient and aluminum nanocomposite can be a good choice to reduce environmental and industrial noise.

This study investigated physiological response of Caspian locust and River red gum affected by Cadmium sulphide nanoparticles. First we grew one-year old seedlings of two plants in similar conditions. The experiment designed was a randomized complete block with three replications and two concentrations of Cadmium Nano-sulphide (20 and 80 mg/lit) beside the control treatment was conducted in greenhouse. After 30 days sampling was done for plants and the amounts of proline, chlorophyll, soluble sugar, Mallon di-aldehyde and amount of Cadmium sulphide in roots and aboveground parts were measured. Analysis of variance was conducted to test differences and Duncan test for compare means. Results showed that more aggregation of nanoparticles was seen in roots than aboveground parts. Also absorption of nanoparticles in River red gum was more than Caspian locust. The results also showed that increasing the amount of nanoparticles affected the amounts of Mallon di-aldehyde. Amount of chlorophyll was decreased in River red gum while inversely was increased in Caspian locust. In general, the concentrations of cadmium sulfide nanoparticles was studied under this experimental conditions, have many negative effects on Caspian locust. Reducing the amount of sugar and proline in this species, is most likely due to the effect of nanoparticles on creating water stress. In fact, nanoparticles have been indirectly reduced the amount of sugar and proline. While River red gum increased production of sugar and proline to regulate osmotic level. However, plant protection and anti-oxidants may also be involved.

The aim of the present study was preparing polymeric materials and nanocomposites based on nanoparticles of zinc oxide in normal cases the coated with gelatin and comparing them in terms of sound transmission loss in different frequencies.
This study was an experimental one. The materials used in the current study were included zinc oxide nanoparticles, polymer polyvinyl chloride (PVC) and the gelatin. The steps of this study were conducted in two phases; the first phase included: making molds and producing nanocomposites and the second phase determining the sound transmission loss in nanocomposites.
Sound transmission loss conventional nanocomposites showed that the highest percentage of the weight in the first and second sound transmission loss in dB in 500 Hz was 4.34. While in the third weight the percent of the nanocomposites, the lowest transmission loss equaled to 0.80 dB at a frequency of 2000 Hz. The results of sound transmission loss in coated gelatin nanocomposites showed that the weight percentage of the first and second was the highest transmission loss equaled to 3.01 dB at a frequency of 500 Hz; and in the third weight percent, the lowest transmission loss equaled to 0.55 dB at a frequency of 2000 Hz, respectively.
Comparing the sound transmission loss in nanocomposites zinc oxide and coated with gelatin normally suggests that the gelatine resulting in increased absorption and sound transmission loss is reduced.

The skin is the largest organ in the body which protects the internal organs against external injury. When the skin is damaged, microorganisms attack easily and cause infection in humans. Many efforts have been made for development of new products to protect the skin from damage and dehydration. Because of the diversity in the wound dressing products, one may possibly be confused when choosing a suitable wound dressing. Although, protection of the wound from infection is one of the reasons for using a wound dressing, acceleration of wound healing process is the main goal. In this paper, we have reviewed several types of modern wound dressings regarding their efficiency in each stage of wound healing. Commercial wound dressings in the form of hydrogels, hydrocolloids, films, foams and nanofibers are introduced and their properties are discussed. Chitosan, alginate and honey are the main common polysaccharides which are usually used as wound dressing materials. The effect of these polysaccharides on wound healing is also described. Finally, the recently developed smart wound dressings which are able to detect the wound infection are presented.

The aim of this study was to evaluate the performance of three nanomotors on 15 transmembrane amyloid-beta precursor proteins by molecular dynamics simulation.
In this study, 15 transmembrane APP (14 mutant forms and one normal) were obtained from National Center for Biotechnology Information (NCBI) and separately modeled in Designer Ascalaph. The synthesized peptides with three-dimensional stable structures were selected to be simulated and then were placed near synthesized nanomotors, hypericin, and retinol using HyperChem. At end of simulation, different parameters such as free energy, intermolecular energy, potential energy, and root mean square displacement (RMSD) were separately obtained for each APP. Finally, the ratio of changes was calculated for each parameter.
The results showed that each nanomotor had different performance on different peptides. Hypericin on peptide 5, retinol on peptide 10, and nanomotors partly on peptide 4 had a great performance. But because each nanomotor influences only special mutant peptide and Alzheimer's disease is caused by different mutations, only one type of nanomotors cannot be used to treat Alzheimer's disease.
According to the investigations performed on the treatment of Alzheimer’s disease, it can be concluded that retinol with three-dimensional structure and dynamic parameters has great performance on transmembrane mutant peptide; therefore, it can be considered as a useful treatment strategy.

Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs).
At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin (βHCG) in the clinical samples was quantified by traditional enzyme-linked immunosorbent assay (ELISA), and then checked by the nano-immunoassay.
The Pearson’s correlation coefficient between ELISA and nano-immunoassay was high, i.e., 0.80. Relative sensitivity and specificity of this nano-immunoassay were reported 97.4% and 96.6%, respectively.
BSA NPs can be applied in nano-immunoassays as a new structure, and as an example, βHCG can be detected by this novel assay.

Using antisense oligonucleotides for targeting essential genes is one of the new methods to control microbial infections. The aim of this study was to investigate four antisense oligonucleotides against rpoD gene of staphylococcus aurous, and to find the probable match in human genes.
First, rpoD gene of staphylococcus aurous was extracted from NCBI (National Center for Biotechnology Information) database. Then, its mRNA sequence was generated and four antisense were selected according to secondary structure of mRNA and thermodynamic parameters. Finally, matching of each selected antisense and all human genome with Nblast algorithm, was evaluated.
This study showed that according to secondary structure and thermodynamic parameters, antisense 1, GAAGAAGTTGGTA, was the best antisense, and had the least Overall ∆G. Matching antisense sequences showed that antisense 1, 2, and 4 had different targets at human mRNA level. But, antisense 3, GAAGCAATTAATT, was ideal and could target only rpoD gene.
Given to secondary structure and thermodynamic parameters, the adequate antisense could be selected for target gene, but most of these antisense targeting other genes in human cells.

Although metal and metal oxide nanoparticles are used in different medical applications, they may have considerable toxicity on various cells, such as myocytes. Therefore, this study aimed to evaluate the toxicity of the naked and serum-treated silver nanoparticles (Ag NPs) and magnesium oxide nanoparticles (MgO NPs) on the cardiomyocytes. Cardiomyocytes were separately exposed to different concentrations of the naked and serum-treated nanoparticles for 24 hours at 37ºC. Then, MTT assay, cell metabolism assay and LDH assay were performed. Naked Ag NPs and MgO NPs had more toxicity than serum-treated nanoparticles. The highest cardiomyocyte toxicity was observed for naked Ag NPs, whereas the minimum toxicity was seen for the serum-treated MgO NPs. Coating of nanoparticles with serum components leads to decrease in toxicity for cardiomyocytes, and MgO NPs have less toxicity on the myocytes than Ag NPs.

Although various surface disinfectants have been introduced, most of them are toxic. The use of natural antimicrobial agent e.g. phytol, extracted from Leptadenia pyrotechnica is a new strategy. The aim of this study was to evaluate the antimicrobial activity, toxicity, and stability of phytol. The serial concentrations of phytol were prepared, and separately incubated with four microbial isolates. Then, its Minimum Inhibitory Concentration (MIC) was measured for each microorganism. For toxicity test, serial concentrations (62.5, 125, 250, 500 and 1000 μg/mL) of phytol were incubated with mouse skin cells, and then cell viability was calculated by MTT assay. For stability test, three common surfaces (stone, steel, and MDF) were considered. Then, 100 μL of phytol was separately spread over their surface, and they have been kept at lab panel for 12, 24 and 36 hours. After incubation, two samples were obtained from each surface and inoculated on nutrient agar plates. Finally, colony count was read for each surface. T-test was used to evaluate the significant differences between groups, and P>0.05 considered as level of significant difference. The MIC50 of phytol against E.coli, C.albicans, and A.niger was 62.5 μg/mL, and against S.aureus was >1000 μg/mL. MTT assay showed that the toxicity of phytol was dose and time dependent. The stability test demonstrated that phytol was stable on the stone, MDF, and steel surfaces until 36 hours. It can be concluded that phytol has high antimicrobial activity, high stability, and low toxicity.

Nanotechnology has opened a new field in medicine as well as in other sciences. The aim of this study was to seek the capability of nanotechnology for the treatment of various reproductive diseases. In this study, we analyzed all articles about “nanotechnology and reproductive medicine” published in 2000-2015, indexed in Google Scholar, PubMed and Science Direct. This study indicated that nanotechnology has been extensively used for different biomedical applications, e.g. detection, drug delivery, diagnostic imaging, etc. particularly in cancer diagnostics and treatment. Here, the emerging uses of nanotechnology in reproductive medicine and reproductive biology were found. The available evidence regarding the use of nanomaterials as experimental tools for the detection and treatment of reproductive diseases was summarized. Nanoparticles have potential applications in reproductive biology. Treatment and imaging of reproductive system-related cancers can be performed by engineered nanoparticles. Also, some non-cancerous diseases can be treated by nanotechnology, e.g. endometriosis. The benefits and concerns associated with their use in a highly delicate system of reproductive tissues and gametes have been investigated. Nano-based methods are innovative and potentially controversial approaches in the clinical settings and give us the mechanisms underlying reproductive diseases.

In spermatogenesis, spermatogonial cells differentiate to the haploid gametes. It has been shown that spermatogenesis can be done at in vitro condition. In vitro spermatogenesis may provide an open window to treat male infertility.

The aim of this study was to evaluate the effects of a novel scaffold containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) on the mouse spermatogonial cell line (SCL).

First, TCP NPs were synthesized by reaction of calcium nitrate and diammonium phosphate at pH 13. Then, serial concentrations of TCP NPs were separately added to 500 mg/mL HSA, and incubated in the 100oC water for 30 min. In the next step, each scaffold was cut (2×2mm), placed into sterile well of microplate, and then incubated for 1, 2, and 3 days at 37oC with mouse SCL. After incubation, the cytotoxicity of the scaffolds was evaluated by different tests including 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) assay, vital staining, and cell counting. On the other hand, the release of TCP NPs and HSA from the scaffolds was measured.

Based on microscopic observation, the size of cavities for all scaffolds was near 200-500 µm, and the size of TCP NPs was near 50-100 nm. All toxicity tests showed that the increase of TCP concentration in the scaffold did not affect mouse SCL. It means that the percentage of cell viability, LDH release, vital cells, and cell quantity was 85%, 105%, 90%, and 110%, respectively. But, the increase of incubation time led to increase of LDH release (up to 115%) and cell count (up to 115%). Also, little decrease of cell viability and vital cells was seen when incubation time was increased. Here, no release of TCP NPs and HSA was seen after increase of TCP concentration and incubation time.

It can be concluded that the increase of TCP concentration in HSA/ TCP NPs scaffold does not lead to cytotoxicity. On the other hand, the increase of incubation time leads to increase of mouse SCL cell death. In this study, it was found that TCP NPs and HSA could not release from the scaffolds. In future, both proliferation and differentiation of mouse SCL on HSA/TCP NPs scaffold must be checked over more wide incubation times.

Some plants show antimicrobial activity against wide range of microorganism. The aim of this study was to investigate the antimicrobial activity and stability of Zataria multiflora Boiss and Satureja khuzestanica essential oil (EO). First, these plants (Zataria multiflora Boiss and Satureja khuzestanica) were collected, verified by a botanist, dried, and grinded. Then, hydro-distallation method was used, to obtain EO from each one. Their antimicrobial activity of each EO was evaluated by micro dilution broth method. For stability test, the surfaces (stone, MDF, and steel) were cleaned by 70% ethanol, and then separately incubated with each EO. Then, all were dried, and incubated for 12, 24 and 36 hours at room temperature. After incubation, sampling was done, inoculated on nutrient agar. Finally, the number of colonies grown on each plate was counted. The MIC50 of both EO was 3.1%. But, the MIC90 of Zataria multiflora Boiss EO against all strains was 12.5%, except Escherichia coli which was 6.2%. Also, the MIC90 of Satureja khuzestanica EO against all strains was 12.5%, except Aspergillus niger which was 6.2%. Both EOs had high stability until 36 hours on the stone and steel. In case of MDF, EOs were stable until 24 hours. No significant difference was observed between stability of Zataria multiflora Boiss and Satureja khuzestanica EO. It can be concluded that Zataria multiflora Boiss and Satureja khuzestanica EO have approximately same antimicrobial activity and stability. Since both EOs have high antimicrobial property, they can be applied to remove microbes from different sources.

Zhumeria majdae is an herbal plant which is only found in Hormozgan province. The aim of this study was to compare the antimicrobial property and toxicity of essential oil (EO) and encapsulated form of the essential oil (EFEO) of Zhumeria majdae. First, the plant was dried, grinded, and its EO extracted by hydrodistallation. Then, the EO was encapsulated by lichitin. Antimicrobial activity of EO and EFEO was evaluated by micro dilution broth method, and minimum inhibitory concentration (MIC) was determined. For toxicity test, serial concentrations of EO and EFEO were incubated with mouse skin cells, and then cell viability was calculated by MTT assay. Based on atomic force microscopy results, the size distribution of EFEO was between 100-500 nm. Antimicrobial test showed that the MIC50 of both EO and EFEO was similar (0.6%). But, in case of Candida albicans, the MIC90 of EO and EFEO was 5% and 1.25%, respectively. It means that EFEO has greater effect on Candida albicans than EO. The results of MTT assay showed that the toxicity of EFEO was less than EO. It was found that in some cases, encapsulation could lead to better antimicrobial property and less toxicity. Because of high antimicrobial activity, both EO and EFEO of Zhumeria majdae may be used as powerfully antimicrobial agents.